| 货号 | MAB2951 |
| 别名 | MGC20461; oncostatin M; oncostatin-M |
| 反应种属 | Human |
| 应用 | Intracellular Staining by Flow Cytometry(2.5 µg/106cells) |
| 目标/特异性 | Detects human Oncostatin M/OSM in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinantmouse Oncostatin M, recombinant human (rh) CLC, rhCNTF, rhCardiotrophin-1,rhIL-6, rhIL-11, or rhLIF is observed. |
| 使用方法 | Intracellular Staining by Flow Cytometry: 2.5 µg/106cells Neutralization: Measured by its ability to neutralize Oncostatin M/OSM-induced proliferation in the TF‑1 human erythroleukemic cell line. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The Neutralization Dose (ND50) is typically 0.3‑1.5 µg/mL in the presence of 2 ng/mL Recombinant Human Oncostatin M/OSM. |
| 来源 | Monoclonal Mouse IgG2A Clone # 17022 |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 5008 (Human); 18413 (Mouse) |
| 纯化方式 | Protein A or G purified from ascites |
| 免疫原 | E. coli-derived recombinant human Oncostatin M/OSM Ala26-Arg221 Accession # P13725 |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Sterile PBS to a final concentration of 0.5 mg/mL. |
| 背景 | Oncostatin M (OSM) is a cytokine originally isolated from medium conditioned by PMA-treated U-937 human histiocytic leukemia cells based on its ability to inhibit growth of A375 melanoma cells. The human OSM cDNA encodes a 252 amino acid pre-pro-OSM polypeptide with a 25 residue hydrophobic signal peptide and a hydrophilic C-terminal domain that are proteolytically processed to generate the 196 residue mature form of OSM. Although both mature and pro-OSM are equally active in radio-receptor assays, the mature OSM is 5- to 60-fold more active in growth inhibition assays. Thus, proteolytic processing of the pro-OSM peptide may be important in regulating the in vivo activities of OSM.
OSM is a pleiotropic cytokine that initiates its biological activities by binding to specific cell surface receptors. The biological activity of human OSM is mediated either by the LIF/OSM receptor complex composed of gp130 and LIF R alpha or by a human OSM specific receptor composed of gp130 and OSM R alpha. The gp130, a signal transducing component ( beta subunit) of the IL-6, LIF and CNTF receptor complexes, was identified as a low-affinity OSM receptor that does not transduce OSM signals. The low affinity LIF receptor (LIF R, a gp130-related protein) has been identified to be a component of a high-affinity OSM receptor that will transduce OSM signals. Besides its growth inhibitory activities on human A375 melanoma and mouse M1 myeloid leukemic cells, as well as on other solid tumor cells, OSM also has growth stimulatory activities on normal fibroblasts, AIDS-Kaposi’s sarcoma cells, and a human erythroleukemia cell line, TF-1. Other OSM-mediated activities reported to date include: stimulation of plasminogen activator activity in cultured bovine aortic endothelial cells; regulation of IL-6 expression in human endothelial cells; and stimulation of LDL uptake and up-regulation of cell surface LDL receptors in HepG2 cells. |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 |
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Detection of Oncostatin M/OSM in Human Dendritic Cells by Flow Cytometry. Human monocyte-derived dendritic cells were stained with Mouse Anti-Human Oncostatin M Monoclonal Antibody (Catalog # MAB2951, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin. | |
Cell Proliferation Induced by Oncostatin M/OSM and Neutralization by Human Oncostatin M/OSM Antibody. Recombinant Human Oncostatin M (Catalog # 295‑OM) stimulates proliferation in the TF‑1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human Oncostatin M (2 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human Oncostatin M Monoclonal Antibody (Catalog # MAB2951). The ND50 is typically 0.3‑1.5 µg/mL. |
