Human FGF R2 (IIIb) MAb (Clone 98707) (500 UG)【科瑞奥博】

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Human FGF R2 (IIIb) MAb (Clone 98707) (500 UG)

货号： MAB665 基本售价： 5026.6 元规格： 500 ug

产品信息

概述
货号	MAB665
别名	BFR-1; CD332 antigen; CD332; CFD1; craniofacial dysostosis 1; EC 2.7.10; EC 2.7.10.1; fibroblast growth factor receptor 2; FLJ98662; Jackson-Weiss syndrome; JWS; Keratinocyte growth factor receptorreceptor like 14; KSAM; K-sam; soluble FGFR4 variant 4
全称	Fibroblast Growth Factor Receptor 2
反应种属	Human
应用	Western Blot(1 µg/mL)
目标/特异性	Reacts with multiple isoforms of human FGF R2 although it shows a marked preference for human FGF R2 (IIIb) isoforms in some assays as described below. In direct ELISAs, displays a three-fold preference for FGF R2 (IIIb) isoforms over (IIIc) isoforms. Shows approximately 50% cross-reactivity with recombinant mouse (rm) FGF R2 isoforms and no cross-reactivity with any isoforms of recombinant human (rh) FGF R1 or rhFGF R3. In Western blots, shows approximately 25% cross-reactivity with rhFGF R2 (IIIc) isoforms, 100% cross-reactivity with all isoforms of rmFGF R2 and no cross-reactivity with any isoforms of rhFGF RI, rhFGF R3, or rhFGF R4. Neutralizes the bioactivity of rhFGF R2 (IIIb) isoforms.
使用方法	Western Blot: 1 µg/mL Neutralization: Measured by its ability to neutralize FGF R2 alpha -mediated inhibition of proliferation in the NR6R‑3T3 mouse fibroblast cell line. The Neutralization Dose (ND₅₀) is typically 2-8 µg/mL in the presence of 6 ng/mL Recombinant Human FGF R2 alpha (IIIb) Fc Chimera, 0.3 ng/mL Recombinant Human FGF acidic, and 10 µg/mL heparin.
来源	Monoclonal Mouse IgG₁ Clone # 98707
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	2263 (Human); 14183 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Translocation of fibroblast growth factor-10 and its receptor into nuclei of human urothelial cells. Authors: Kosman J, Carmean N, Leaf EM, Dyamenahalli K, Bassuk JA J. Cell. Biochem., 2007;102(3):769-85. Species: Human Sample Type: Whole Cells Application: ICC
纯化方式	Protein A or G purified from ascites
免疫原	Mouse myeloma cell line NS0-derived recombinant human FGF R2 isoforms and S. frugiperda insect ovarian cell line Sf 21-derived recombinant human FGF R2 isoforms
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	FGF R2 is a transmembrane tyrosine kinase that functions as a receptorfor several FGF family proteins. The extracellular domain includesthree ( alpha isoforms) or two ( beta isoforms) Ig-like domains. Alternativeexon usage results in IIIb or IIIc isoforms that differ in theirmembrane proximal Ig-like domain.
运输条件	Blue Ice
存放说明	-20℃
参考文献	FGF Family FGF Ligands FGF Regulators Fibroblast Growth Factor Superfamily Fibroblast Growth Factors and their Receptors Glial Lineage Markers Human CD Marker Products ICC / IHC Images: FGF R2 Mesenchymal to Epithelial Transition Mini-review: Fibroblast Growth Factors (FGFs) Neural Stem Cell Markers Receptor Tyrosine Kinases (RTKs) Receptor Tyrosine Kinases (RTKs) in the Akt Pathway Receptors in the Jak/STAT Pathway

参考图片

FGF R2 alpha Inhibition of FGF acidic-dependent Cell Proliferation and Neutralization by Human FGF R2 Antibody. Recombinant Human FGF R2 alpha (IIIb) Fc Chimera (Catalog # 663-FR) inhibits Recombinant Human FGF acidic (Catalog # 232-FA) induced proliferation in the NR6R‑3T3 mouse fibroblast cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human FGF acidic (0.3 ng/mL) activity elicited by Recombinant Human FGF R2 alpha (IIIb) Fc Chimera (6 ng/mL) is neutralized (green line) by increasing concentrations of Human FGF R2 (IIIb) Monoclonal Antibody (Catalog # MAB665). The ND₅₀ is typically 2-8 µg/mL in the presence of heparin (10 µg/mL).