Human IFN-gamma R1 MAb (Clone 92101) (500 UG)【科瑞奥博】

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Human IFN-gamma R1 MAb (Clone 92101) (500 UG)

货号： MAB6731 基本售价： 5026.6 元规格： 500 ug

产品信息

概述
货号	MAB6731
别名	AVP, type 2; CD119 antigen; CD119; FLJ45734; IFN-gamma receptor 1; IFN-gamma-R1; IFNGR; IFNGR1; immune interferon receptor 1; interferon gamma receptor 1; interferon-gamma receptor alpha chain; type 2
全称	Interferon gamma Receptor 1
反应种属	Human
应用	Western Blot(1 µg/mL) Flow Cytometry(2.5 µg/10⁶cells)
目标/特异性	Detects human IFN-gamma R1 in direct ELISAs and Western blots. In direct ELISAs, this antibody does not cross-react with recombinant mouse IFN‑ gamma R1, recombinant human (rh) IFN-gamma R2, or rhIL-10 R beta.
使用方法	Western Blot: 1 µg/mL Flow Cytometry: 2.5 µg/10⁶cells Neutralization: Measured by its ability to neutralize IFN‑ gamma R1/CD119-mediated inhibition of EMCV-induced cytopathy in the HeLa human cervical epithelial carcinoma cell line. Meager, A. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 129. The Neutralization Dose (ND₅₀) is typically 0.01-0.03 µg/mL in the presence of 2 ng/mL Recombinant Human IFN‑ gamma.
来源	Monoclonal Mouse IgG₁ Clone # 92101
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	3459 (Human); 15979 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Induction of indoleamine 2,3-dioxygenase by Borrelia burgdorferi in human immune cells correlates with pathogenic potential. Authors: Love A, Schwartz I, Petzke M J Leukoc Biol, 2015;97(2):379-90. Species: Human Sample Type: Whole Cells Application: Neut Interferon-gamma differentially regulates TGF-beta1 and TGF-beta2 expression in human retinal pigment epithelial cells through JAK-STAT pathway. Authors: Nagineni CN, Cherukuri KS, Kutty V, Detrick B, Hooks JJ J. Cell. Physiol., 2007;210(1):192-200. Species: Human Sample Type: Whole Cells Application: Neut Human intestinal intraepithelial lymphocytes keep TNF alpha levels low by cell uptake and feedback inhibition of transcription. Authors: Ebert EC, Mehta V Cell. Immunol., 2006;241(1):7-13. Species: Human Sample Type: Whole Cells Application: Flow
纯化方式	Protein A or G purified from ascites
免疫原	Mouse myeloma cell line NS0-derived recombinant human IFN-gamma R1 Glu18-Gly245 Accession # P15260.1
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	The high-affinity IFN-gamma receptor complex is made up of two type I membrane proteins, IFN-gamma R1 (IFN-gamma R alpha ) and IFN-gamma R2 (IFN-gamma R beta ). Both proteins are members of the type II cytokine receptor family and share approximately 52% amino acid sequence identity. IFN-gamma R1 is the ligand-binding subunit that is necessary and sufficient for IFN-gamma binding and receptor internalization. IFN-gamma R2 is required for IFN-gamma signaling but does not bind IFN-gamma by itself. Human IFN-gamma R1 cDNA encodes a 499 amino acid (aa) residue protein with a 17 aa signal peptide, a 228 aa extracellular domain, a 23 aa transmembrane domain, and a 221 aa intracellular domain. Human and mouse IFN-gamma R1 share 52% amino acid sequence identity and bind IFN-gamma in a species-specific manner. IFN-gamma R1 is constitutively expressed in most cell types. Soluble IFN-gamma R1 that binds IFN-gamma has been detected in biological fluids.
运输条件	Blue Ice
存放说明	-20℃
参考文献	Bach, E.A.et al. (1997) Annu. Rev. Immunol. 15:563.

参考图片

IFN‑ gamma Inhibition of EMCV-induced Cytopathy and Neutralization by Human
IFN‑ gamma R1/CD119 Antibody. Recombinant Human IFN‑ gamma (Catalog # 285-IF) reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the HeLa human cervical epithelial carcinoma cell line in a dose-dependent manner (orange line). Inhibition of EMCV activity elicited by Recombinant Mouse Anti-Human IFN‑ gamma (2 ng/mL) is neutralized (green line) by increasing concentrations of Human IFN‑ gamma R1/CD119 Monoclonal Antibody (Catalog # MAB6731). The ND₅₀ is typically 0.01-0.03 µg/mL.