| 货号 | MAB630 |
| 别名 | Antagonist decoy receptor for TRAIL/Apo-2L; CD263 antigen; CD263; cytotoxic TRAIL receptor-3; DcR1; DCR1MGC149502; Decoy receptor 1; Decoy TRAIL receptor without death domain; LITMGC149501; Lymphocyte inhibitor of TRAIL; TNFRSF10C; TRAIL receptor 3; TRAIL receptor without an intracellular domain; TRAIL-R3; TRAILR3TNF-related apoptosis-inducing ligand receptor 3; TRIDDCR1-TNFR; tumor necrosis factor receptor superfamily member 10C; tumor necrosis factor receptor superfamily, member 10c, decoy without anintracellular domain | 全称 | TRAIL Receptor 3 |
| 反应种属 | Human |
| 应用 | Neutralization |
| 目标/特异性 | Detects human TRAIL R3/TNFRSF10C in direct ELISAs and Western blots. In direct ELISAs, approximately 20%‑40% cross-reactivity with recombinant human (rh) TRAIL R1 is observed and no cross-reactivity with rhTRAIL R2 or rhTRAIL R4 is observed. |
| 使用方法 | Neutralization: Measured by its ability to neutralize TRAIL R3/TNFRSF10C-mediated inhibition of cytotoxicity in the L‑929 mouse fibroblast cell line. The Neutralization Dose (ND50) is typically 0.02-0.08 µg/mL in the presence of 10 ng/mL Recombinant Human TRAIL R3/TNFRSF10C Fc Chimera, 20 ng/mL of Recombinant Human TRAIL/TNFSF10, and 1 µg/mL actinomycin D. |
| 来源 | Monoclonal Mouse IgG1 Clone # 90903 |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 8794 (Human) |
| 纯化方式 | Protein A or G purified from ascites |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant human TRAIL R3/TNFRSF10C Ala26-Ala221 Accession # O14798 |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | TRAIL R3, also called TNFRSF10C, DcR1 (decoy receptor 1), LIT, and TRID, is a glycosylphosphatidylinositol-linked membrane protein which binds TRAIL (Apo2 Ligand) with high affinity (1, 2). TRAIL R3 contains a TRAIL-binding extracellular cysteine-rich domain but lacks an intracellular signaling domain. As a result, binding of TRAIL to TRAIL R3 does not trigger a pro-apoptotic signal. TRAIL R3 has been shown to protect cells bearing TRAIL R1 and/or TRAIL R2 from TRAIL-induced apoptosis. |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 |
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TRAIL R3/TNFRSF10C Inhibition of TRAIL/TNFSF10-induced Cytotoxicity and Neutralization by Human TRAIL R3/TNFRSF10C Antibody. In the presence of the metabolic inhibitor actinomycin D (1 µg/mL), Recombinant Human TRAIL R3/TNFRSF10C Fc Chimera (Catalog # 630-TR) inhibits Recombinant Human TRAIL/TNFSF10 (Catalog # 375-TEC) induced cytotoxicity in the L‑929 mouse fibroblast cell line in a dose-dependent manner (orange line). Under these conditions, inhibition of Recombinant Human TRAIL/TNFSF10 (20 ng/mL) activity elicited by Recombinant Human TRAIL R3/TNFRSF10C Fc Chimera (10 ng/mL) is neutralized (green line) by increasing concentrations of Human TRAIL R3/TNFRSF10C Monoclonal Antibody (Catalog # MAB630). The ND50 is typically 0.02-0.08 µg/mL. |
