| 货号 | MAB3631 |
| 别名 | chemokine (C-C motif) ligand 15; Chemokine CC-2; HCC-2; HCC-2Lkn-1; Leukotactin 1; Leukotactin-1; LKN1; LKN-1; Macrophage inflammatory protein 5; MIP-1 delta; MIP5; MIP-5; MIP-5MIP-1d; Mrp-2b; NCC-3; NCC-3MIP-1D; NCC3MRP-2B; new CC chemokine 3; SCYA15member 15; Small-inducible cytokine A15 |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects human CCL15/MIP‑1δ in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant humanCCL1, 2, 3, 4, 5, 7, 8, 11, 13, 14, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26,recombinant mouse CCL1, 2, 3, 4, 5, 6, 7, 9, 11, 12, 17, 19, 20, 21, 22, 24,25, or recombinant rat CCL20 is observed. |
| 使用方法 | Western Blot: 1 µg/mL Neutralization: Measured by its ability to neutralize CCL15/MIP‑1δ-induced chemotaxis in the THP‑1 human acute monocytic leukemia cell line. The Neutralization Dose (ND50) is typically 5-20 µg/mL in the presence of 10 ng/mL Recombinant Human CCL15/MIP‑1δ 68 aa. |
| 来源 | Monoclonal Mouse IgG1 Clone # 88119 |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 6359 (Human) |
| 纯化方式 | Protein A or G purified from ascites |
| 免疫原 | E. coli-derived recombinant human CCL15/MIP-1δ Ser46-Ile113 Accession # Q16663 |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | CCL15, also called macrophage inflammatory protein 1 delta (MIP-1δ and Leukotactin-1 (LKN-1), is a CC chemokine with two additional cysteine residues that form a third disulfide bond. CCL15 functions as a chemoattractant for monocytes, T cells, and eosinophils through interactions with CCR1. CCL15 cDNA encodes a predicted 113 amino acid (aa) protein containing a putative signal peptide of 21 aa that is cleaved to generate a 92 aa residue mature protein. Within the CC family members, human CCL15 shares 45%, 44%, 35%, and 30% aa homology with mouse C10, human MPIF-1, human HCC-1, and mouse MIP-1 gamma, respectively. The gene for MIP-1δ is found on chromosome 17 where the genes for most of the human CC chemokines are located. Human CCL15 is expressed in T and B lymphocytes, NK cells, monocytes and monocyte-derived dendritic cells. |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 |
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Chemotaxis Induced by CCL15/MIP‑1δ and Neutralization by Human CCL15/ MIP‑1δ Antibody. Recombinant Human CCL15/ MIP‑1δ (Catalog # 628-LK) chemoattracts the THP‑1 human acute monocytic leukemia cell line in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin. Chemotaxis elicited by Recombinant Human CCL15/ MIP‑1δ (10 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti‑Human CCL15/MIP‑1δ Monoclonal Antibody (Catalog # MAB3631). The ND50 is typically 5-20 µg/mL. |
