Rat GM-CSF MAb (Clone 83308) (500 UG)【科瑞奥博】

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Rat GM-CSF MAb (Clone 83308) (500 UG)

货号： MAB5181 基本售价： 5026.6 元规格： 500 ug

产品信息

概述
货号	MAB5181
别名	colony stimulating factor 2 (granulocyte-macrophage); Colony-stimulating factor; CSF; CSF2; GM-CSF; GMCSFgranulocyte-macrophage colony-stimulating factor; granulocyte-macrophage colony stimulating factor; MGC131935; MGC138897; molgramostin; sargramostim
全称	Granulocyte Macrophage Growth Factor
反应种属	Rat
应用	Western Blot(1 µg/mL) Immunocytochemistry(8-25 µg/mL) Intracellular Staining by Flow Cytometry(2.5 µg/10⁶cells)
目标/特异性	Detects rat GM-CSF in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant GM‑CSF from mouse, human, or pig is observed.
使用方法	Western Blot: 1 µg/mL Immunocytochemistry: 8-25 µg/mL Intracellular Staining by Flow Cytometry: 2.5 µg/10⁶cells Neutralization: Measured by its ability to neutralize GM‑CSF-induced proliferation in the DA3 mouse myeloma cell line. Ihle, J. N. et al. (1984) Advances in Viral Oncology. In G. Klein (eds): Raven Press, New York, NY. 4:95. The Neutralization Dose (ND₅₀) is typically 1-4 µg/mL in the presence of 0.5 ng/mL Recombinant Rat GM‑CSF.
来源	Monoclonal Mouse IgG_2B Clone # 83308
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	1437 (Human); 12981 (Mouse); 116630 (Rat); 397208 (Porcine); 403923 (Canine); 493805 (Feline)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Aging diminishes the resistance of AO rats to EAE: putative role of enhanced generation of GM-CSF Expressing CD4+ T cells in aged rats. Authors: Stojic-Vukanic Z, Nacka-Aleksic M, Pilipovic I, Vujnovic I, Blagojevic V, Kosec D, Dimitrijevic M, Leposavic G Immun Ageing, 2015;12(0):16. Species: Rat Sample Type: Whole Cells Application: Flow Effect of Lactobacillus acidophilus supernatants on body weight and leptin expression in rats. Authors: Sousa R, Halper J, Zhang J BMC Complement Altern Med, 2008;8(0):5. Species: Rat Sample Type: Whole Tissue Application: IHC Paraffin-embedded
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	E. coli-derived recombinant rat GM-CSF Ala1-Lys127 Accession # P48750
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Rat
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	GM-CSF was initially characterized as a factor that can support the in vitro colony formation of granulocyte-macrophage progenitors. It is also a growth factor for erythroid, megakaryocyte, and eosinophil progenitors. GM-CSF is produced by a number of different cell types (including T cells, B cells, macrophages, mast cells, endothelial cells, fibroblasts, and adipocytes) in response to cytokine or inflammatory stimuli. On mature hematopoietic cells, GM-CSF is a survival factor for and activates the effector functions of granulocytes, monocytes/macrophages, and eosinophils (1, 2). GM-CSF promotes a Th1 biased immune response, angiogenesis, allergic inflammation, and the development of autoimmunity (3‑5). It shows clinical effectiveness in ameliorating chemotherapy-induced neutropenia, and GM-CSF transfected tumor cells are utilized as cancer vaccines (6, 7). The 22 kDa glycosylated GM-CSF, similar to IL‑3 and IL‑5, is a cytokine with a core of four bundled alpha ‑helices (8‑10). Mature rat GM-CSF shares 56%‑69% amino acid sequence identity with canine, feline, human, mouse, and porcine GM‑CSF. GM‑CSF exerts its biological effects through a heterodimeric receptor complex composed of GM‑CSF R alpha /CD116 and the signal transducing common beta chain (CD131) which is also a component of the high-affinity receptors for IL-3 and IL-5 (11, 12). In addition, GM-CSF binds a naturally occurring soluble form of GM‑CSF R alpha (13). Rat GM‑CSF is active on mouse cells, although mouse GM‑CSF is only weakly active on rat cells (14, 15).
运输条件	Blue Ice
存放说明	-20℃
参考文献	Martinez-Moczygemba, M. and D.P. Huston (2003) J. Allergy Clin. Immunol. 112:653. Barreda, D.R. et al. (2004) Dev. Comp. Immunol. 28:509. Eksioglu, E.A. et al. (2007) Exp. Hematol. 35:1163. Cao, Y. (2007) J. Clin. Invest. 117:2362. Fleetwood, A.J. et al. (2005) Crit. Rev. Immunol. 25:405. Heuser, M. et al. (2007) Semin. Hematol. 44:148. Hege, K.M. et al. (2006) Int. Rev. Immunol. 25:321. Kaushansky, K. et al. (1992) Biochemistry 31:1881. Diederichs, K. et al. (1991) Science 254:1779. Smith, L.R. et al. (1994) Immunogenetics 39:80. Onetto-Pothier, N. et al. (1990) Blood 75:59. Hayashida, K. et al. (1990) Proc. Natl. Acad. Sci. 87:9655. Pelley, J.L. et al. (2007) Exp. Hematol. 35:1483. Oaks, M.K. et al. (1995) J. Interferon Cytokine Res. 15:1095. Vandenabeele, P. et al. (1990) Lymphokine Res. 9:381.

参考图片

Cell Proliferation Induced by GM‑CSF and Neutralization by Rat GM‑CSF Antibody. Recombinant Rat GM‑CSF (Catalog # 518-GM) stimulates proliferation in the DA3 mouse myeloma cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Rat GM‑CSF (0.5 ng/mL) is neutralized (green line) by increasing concentrations of Rat GM‑CSF Monoclonal Antibody (Catalog # MAB5181). The ND₅₀ is typically 1-4 µg/mL.

GM‑CSF in Rat Splenocytes. GM‑CSF was detected in immersion fixed rat splenocytes using Rat GM‑CSF Monoclonal Antibody (Catalog # MAB5181) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.