| 货号 | MAB540 |
| 描述 | For ELISA the Antibody Pairs information：Capture antibody：MAB540；Detection antibody：BAF540; and protein: 540-RM-025 |
| 别名 | beta chemokine exodus-1; Beta-chemokine exodus-1; CC chemokine LARC; C-C motif chemokine 20; chemokine (C-C motif) ligand 20; CKb4; exodus-1; LARC; LARCLiver and activation-regulated chemokine; MIP-3 alpha; MIP-3a; MIP-3-alpha; MIP3AMacrophage inflammatory protein 3 alpha; SCYA20Small-inducible cytokine A20; small inducible cytokine subfamily A (Cys-Cys), member 20; ST38 |
| 反应种属 | Rat |
| 应用 | Western Blot(1 µg/mL) ELISA Capture (Matched Antibody Pair)(2-8 µg/mL ) ELISA Detection (Matched Antibody Pair)(0.1-0.4 µg/mL ) ELISA Standard ( ) |
| 目标/特异性 | Detects rat CCL20/MIP-3 alpha in ELISAs and Western blots. |
| 使用方法 | Western Blot: 1 µg/mL ELISA Capture (Matched Antibody Pair): 2-8 µg/mL ELISA Detection (Matched Antibody Pair): 0.1-0.4 µg/mL ELISA Standard : Neutralization: Measured by its ability to neutralize CCL20/MIP‑3 alpha -induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR6. The Neutralization Dose (ND50) is typically 20-40 µg/mL in the presence of 5 µg/mL Recombinant Rat CCL20/MIP‑3 alpha. |
| 来源 | Monoclonal Mouse IgG1 Clone # 83225 |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 6364 (Human); 20297 (Mouse); 29538 (Rat) |
| 纯化方式 | Protein A or G purified from ascites |
| 免疫原 | E. coli-derived recombinant rat CCL20/MIP-3 alpha Ala26-Met96 Accession # P97884.1 |
| 内毒素水平 | <0.20 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Rat |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | CCL20, also known as MIP-3 alpha, LARC (Liver and Activation-regulated Chemokine) and as Exodus, is one of many novel beta chemokines identified through bioinformatics. Rat CCL20 cDNA encodes a 96 amino acid (aa) precursor protein with a 25 aa putative signal peptide that is predicted to be cleaved to form the 71 aa mature secreted protein. CCL20 is distantly related to other beta chemokines (20-28% aa sequence identity). Rat MIP-3 alpha shares approximately 70% and 61% aa sequence homology with mouse and human CCL20, respectively. CCL20 has been shown to be expressed predominantly in lymph nodes, appendix, PBL, fetal liver, fetal lung, and epithelial cells of intestinal tissues. The expression of CCL20 is strongly up-regulated by inflammatory signals and down-regulated by the anti-inflammatory cytokine IL-10. Synthetic or recombinant CCL20 has been shown to be chemotactic for lymphocytes and dendritic cells, and inhibits proliferation of myeloid progenitors in colony formation assays. CCL20 has now been shown to be a unique functional ligand for CCR-6 (previously referred to as GPR-CY4, CKR-L3, or STRL22 orphan receptor), a chemokine receptor that is selectively and highly expressed in human dendritic cells derived from CD34+ cord blood precursors. |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 |
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Chemotaxis Induced by CCL20/MIP‑3 alpha and Neutralization by Rat CCL20/MIP‑3 alpha Antibody. Recombinant Rat CCL20/MIP‑3 alpha (Catalog # 540-RM) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR6 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Rat CCL20/MIP‑3 alpha (5 µg/mL) is neutralized (green line) by increasing concentrations of Rat CCL20/MIP‑3 alpha Monoclonal Antibody (Catalog # MAB540). The ND50 is typically 20-40 µg/mL. |
