| 货号 | MAB6071 |
| 别名 | DKFZp434C1418; EC 2.7.10; EC 2.7.10.1; Ehk2; EHK-2; EPA6; EPH homology kinase 2; EPH receptor A6; ephrin receptor EphA6; ephrin type-A receptor 6; FLJ35246; Hek12; PRO57066 | 全称 | Eph Receptor A6 |
| 反应种属 | Mouse |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects mouse EphA6 in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross‑reactivity with recombinant rat EphB1 is observed and no cross-reactivity with recombinant human EphA1, A5, recombinant mouse EphA2, A3, A4, A7, A8, B2, B3, B4, or B6 is observed. |
| 使用方法 | Western Blot: 1 µg/mL Blockade of Receptor-ligand Interaction: In a functional ELISA, 0.05-0.15 µg/mL of this antibody will block 50% of the binding of 20 ng/mL of biotinylated Recombinant Human Ephrin A3 to immobilized Recombinant Mouse EphA6 Fc Chimera (Catalog # 607-A6) coated at 2 µg/mL (100 µL/well). At 1 μg/mL, this antibody will block >90% of the binding. |
| 来源 | Monoclonal Rat IgG1 Clone # 81605 |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 285220 (Human); 13840 (Mouse) |
| 纯化方式 | Protein A or G purified from ascites |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant mouse EphA6 Met1-Gln546 Accession # Q62413 |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | Cell migration is influenced by chemodirectants including a large family of receptor tyrosine kinases collectively referred to as Ephs. Ephs are divided into two classes based on their extracellular structure and ligand specificity. Eph receptors that bind preferentially to ephrin-A ligands are referred to as EphA. All Eph receptors contain an extracellular region consisting of a globular domain, a cysteine-rich domain, and two fibronectin type III domains. This is followed by the transmembrane region and cytoplasmic region. The cytoplasmic region contains a juxtamembrane motif with two tyrosine residues, a kinase domain, and a conserved sterile alpha motif (SAM) in the carboxy tail with one conserved tyrosine residue. |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 |
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