| 货号 | MAB328 |
| 描述 | For ELISA the Antibody Pairs information：Capture antibody：MAB328；Detection antibody：BAF802; and protein: 802-HC-025 |
| 别名 | C-C motif chemokine 16; chemokine (C-C motif) ligand 16; Chemokine CC-4; Chemokine LEC; CKb12; HCC-4; HCC-4Liver-expressed chemokine; LCC-1Lymphocyte and monocyte chemoattractant; LEC; liver CC chemokine-1; LMC; LMCSmall-inducible cytokine A16; monotactin-1; Mtn-1; NCC-4; NCC4IL-10-inducible chemokine; NCC-4ILINCK; new CC chemokine 4; SCYA16MGC117051; SCYL4; small inducible cytokine subfamily A (Cys-Cys), member 16 |
| 反应种属 | Human |
| 应用 | Western Blot,Immunohistochemistry,CyTOF-ready,Intracellular Staining by Flow Cytometry,ELISA Capture (Matched Antibody Pair),ELISA Detection (Matched Antibody Pair),ELISA Standard ,Neutralization |
| 目标/特异性 | Detects human CCL16/HCC‑4 in direct ELISAs and Western blots. |
| 使用方法 | Western Blot: 1 µg/mL Immunohistochemistry: 8-25 µg/mL Intracellular Staining by Flow Cytometry: 0.25 µg/106cells ELISA Capture (Matched Antibody Pair): 2-8 µg/mL ELISA Detection (Matched Antibody Pair): 0.1-0.4 µg/mL ELISA Standard : Neutralization: Measured by its ability to neutralize CCL16/HCC‑4-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR1. The Neutralization Dose (ND50) is typically 5-20 µg/mL in the presence of 0.75 µg/mL Recombinant Human CCL16/HCC‑4. |
| 来源 | Monoclonal Mouse IgG1 Clone # 70218 |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 6360 (Human) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. CCL16/LEC powerfully triggers effector and antigen-presenting functions of macrophages and enhances T cell cytotoxicity. | |
| 纯化方式 | Protein A or G purified from ascites |
| 免疫原 | E. coli-derived recombinant human CCL16/HCC-4 Gln24-Gln120 Accession # O15467 |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | Human HCC-4, also named NCC-4, liver-expressed chemokine (LEC), and lymphocyte and monocyte chemoattractant (LMC), is a novel CC chemokine identified through bioinformatics. HCC-4 cDNA encodes a 120 amino acid (aa) residue precursor protein with a 23 aa residue predicted signal peptide that is cleaved to generate a 97 aa residue mature protein. HCC-4 is distantly related to other CC chemokines, exhibiting less than 30% aa sequence identity. Among these CC chemokines, HCC-4 has the most similarity to HCC-1. Two potential polyadenylation signals are present on the human HCC-4 gene, and as a result, two transcripts containing approximately 1,500 base pairs and 500 base pairs have been detected. HCC-4 is expressed weakly by some lymphocytes, including NK cells, gamma δ T cells, and some T cell clones. The expression of HCC-4 in monocytes is highly upregulated in the presence of IL-10. The HCC-4 gene has been mapped to chromosome 17q where multiple CC chemokines are clustered. Recombinant HCC-4 has been shown to chemoattract human monocytes and THP-1 cells but not resting lymphocytes or neutrophils. HCC-4 has also been found to suppress proliferation of myeloid progenitor cells. The HCC-4 induced calcium flux in THP-1 cells can be desensitized by prior exposure to RANTES, suggesting that HCC-4 and RANTES share the same receptor in THP-1 cells. |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 |
|
Chemotaxis Induced by CCL16/HCC‑4 and Neutralization by Human CCL16/ HCC‑4 Antibody. Recombinant Human CCL16/ HCC‑4 (Catalog # 802‑HC) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR1 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL16/ HCC‑4 (0.75 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CCL16/ HCC‑4 Monoclonal Antibody (Catalog # MAB328). The ND50 is typically 5‑20 µg/mL. |
