Human APRIL/TNFSF13 MAb (Clone 670820) (500 UG)【科瑞奥博】

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Human APRIL/TNFSF13 MAb (Clone 670820) (500 UG)

货号： MAB5860 基本售价： 5026.6 元规格： 500 ug

产品信息

概述
货号	MAB5860
别名	A proliferation-inducing ligand; APRILFLJ57090; CD256 antigen; CD256; TALL2; TALL-2; TNF- and APOL-related leukocyte expressed ligand 2; TNF-related death ligand 1; TNFSF13; TRDL1; TRDL-1; tumor necrosis factor (ligand) superfamily, member 13; tumor necrosis factor ligand superfamily member 13; tumor necrosis factor-like protein ZTNF2; tumor necrosis factor-related death ligand-1; ZTNF2
全称	A Proliferation-inducing Ligand
反应种属	Human
应用	Neutralization
目标/特异性	Detects human APRIL/TNFSF13 in direct ELISAs. In direct ELISAs, approximately 10%cross-reactivity with recombinant mouse APRIL is observed, and nocross-reactivity with recombinant human BAFF is observed.
使用方法	Neutralization: Measured by its ability to neutralize APRIL/TNFSF13-induced proliferation in mouse B cells. The Neutralization Dose (ND₅₀) is typically 0.15-0.75 ug/mL in the presence of 200 ng/mL Recombinant Human APRIL/TNFSF13.
来源	Monoclonal Mouse IgG₁ Clone # 670820
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	8741 (Human); 69583 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Nurse-like cells promote CLL survival through LFA-3/CD2 interactions Oncotarget, 2016;0(0):. Species: Human Sample Type: Whole Cells Application: Neut Selective regulation of axonal growth from developing hippocampal neurons by tumor necrosis factor superfamily member APRIL. Authors: Osorio C, Chacon P, White M, Kisiswa L, Wyatt S, Rodriguez-Tebar A, Davies A Mol Cell Neurosci, 2014;59(0):24-36. Species: Human Sample Type: Whole Cells Application: Neut
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	Human embryonic kidney cell line HEK293-derived recombinant human APRIL/TNFSF13 Ala105-Leu250 Accession # O75888
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Sterile PBS to a final concentration of 0.5 mg/mL.
背景	APRIL (a proliferation inducing ligand), also known as TNFSF13, TALL2, and TRDL1, is a member of the TNF ligand superfamily. It is synthesized as a 32 kDa type II transmembrane protein which is cleaved by furin in the Golgi to release a 17 kDa soluble molecule. Secreted APRIL consists almost entirely of a single TNF homology domain. Little or no transmembrane APRIL is expressed on the cell surface. Alternate splicing generates isoforms with short deletions at the N- or C‑terminus. Both APRIL and the closely related protein BAFF signal through the TNF superfamily receptors TACI and BCMA to promote cellular proliferation and protect from apoptosis. APRIL can form bioactive heterotrimers with BAFF. A bioactive cell surface-expressed protein known as TWEPRIL consists of the intracellular domain, transmembrane segment, and stalk region of TWEAK fused to the TNF homology domain of APRIL. Human APRIL shares 85% amino acid sequence identity with mouse and rat APRIL.
运输条件	Blue Ice
存放说明	-20℃
参考文献	BAFF and APRIL Involvement in B Cell Development Cancer Biomarkers Human CD Marker Products Regulation of Apoptosis by TNF Superfamily Members Regulation of B Cell Activation and Homeostasis by TNF Superfamily Members TNF Superfamily TNF Superfamily Ligands

参考图片

Cell Proliferation Induced by APRIL/TNFSF13 and Neutralization by Human APRIL/TNFSF13 Antibody. Recombinant Human APRIL/TNFSF13 induces proliferation in mouse B cells in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Human APRIL/TNFSF13 (200 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human APRIL/TNFSF13 Monoclonal Antibody (Catalog # MAB5860). The ND₅₀ is typically
0.15-0.75 µg/mL.