Human CCL28 MAb (Clone 62705) (500 UG)【科瑞奥博】

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Human CCL28 MAb (Clone 62705) (500 UG)

货号： MAB717 基本售价： 5026.6 元规格： 500 ug

产品信息

概述
货号	MAB717
别名	CCL28; chemokine (C-C motif) ligand 28; MEC; member 28; MGC71902; Protein CCK1; VIC
反应种属	Human
应用	Western Blot(1 µg/mL)
目标/特异性	Detects human CCL28 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human CCL1, 2, 3, 4, 5, 7, 8, 11,12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, recombinant mouse CCL1,2, 3, 4, 5, 6, 7, 9, 11, 19, 21, 22, 25, or recombinant rat CCL20 is observed.
使用方法	Western Blot: 1 µg/mL Neutralization: Measured by its ability to neutralize CCL28-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with mouse CCR10. The Neutralization Dose (ND₅₀) is typically 15-45 µg/mL in the presence of 4 µg/mL Recombinant Human CCL28.
来源	Monoclonal Mouse IgG₁ Clone # 62705
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	56477 (Human); 56838 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Tumour hypoxia promotes tolerance and angiogenesis via CCL28 and T(reg) cells. Authors: Facciabene A, Peng X, Hagemann IS, Balint K, Barchetti A, Wang LP, Gimotty PA, Gilks CB, Lal P, Zhang L, Coukos G Nature, 2011;475(7355):226-30. Species: Human Sample Type: Whole Cells Application: Neut Epithelial inflammation is associated with CCL28 production and the recruitment of regulatory T cells expressing CCR10. Authors: Eksteen B, Miles A, Curbishley SM, Tselepis C, Grant AJ, Walker LS, Adams DH J. Immunol., 2006;177(1):593-603. Species: Human Sample Type: Tissue Homogenates Application: WB CCL28 has dual roles in mucosal immunity as a chemokine with broad-spectrum antimicrobial activity. Authors: Hieshima K, Ohtani H, Shibano M, Izawa D, Nakayama T, Kawasaki Y, Shiba F, Shiota M, Katou F, Saito T, Yoshie O J. Immunol., 2003;170(3):1452-61. Species: Human Sample Type: Milk Application: ELISA Development
纯化方式	Protein A or G purified from ascites
免疫原	E. coli-derived recombinant human CCL28 Ile23-Tyr127 Accession # Q9NRJ3
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	Human CCL28 (CC chemokine ligand 28) is a 127 amino acid (aa) precursor protein with a putative 22 aa signal peptide that is cleaved to produce the 105 aa residue mature protein. Mature human and mouse CCL28 share 83% aa sequence identity. Among CC chemokines, CCL28 shares the most homology with CCL27/CTACK. The mouse CCL28 gene has been mapped to the distal region of chromosome 13. Human and mouse CCL28 RNA expression is highest in epithelial cells of normal and pathologic colon. Human CCL28 RNA is also present in normal and asthmatic lung tissues. CCR10 (GPR2 orphan receptor) is the receptor for both CCL27/CTACK and CCL28.
运输条件	Blue Ice
存放说明	-20℃
参考文献	Wang, W.et al. (2000) J. Biol. Chem. 275:22313.

参考图片

Chemotaxis Induced by CCL28 and Neutralization by Human CCL28 Antibody. Recombinant Human CCL28 (Catalog # 717-VC) chemoattracts the BaF3 mouse pro‑B cell line transfected with mouse CCR10 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin. Chemotaxis elicited by Recombinant Human CCL28 (4 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CCL28 Monoclonal Antibody (Catalog # MAB717). The ND₅₀ is typically 15‑45 µg/mL.