| 货号 | MAB5670 |
| 别名 | APC1 protein; Cachectin; Cachetin; DIF; TNF; TNF, monocyte-derived; tnfa; tnf-a; TNF-alphacachectin; TNFATNF, macrophage-derived; TNFSF1A; TNFSF2; TNFSF2TNF superfamily, member 2; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor alpha; Tumor necrosis factor ligand superfamily member 2; tumor necrosis factor; tumor necrosis factor-alpha | 全称 | Tumor Necrosis Factor alpha |
| 应用 | Neutralization |
| 目标/特异性 | Detects rabbit TNF-alpha in direct ELISAs. In direct ELISAs, no cross-reactivitywith recombinant TNF-alpha from cotton rat, cat, cow, dog, guinea pig, horse,human, mouse, pig, rat, or rhesus is observed. No cross-reactivity withrecombinant human (rh) 4-1BB Ligand, rhAPRIL, rhBAFF, rhEDA, rhEDA-A2, rhFasLigand, rhGITR Ligand, rhLIGHT, rhLymphotoxin alpha 1 beta 2, rhLymphotoxin alpha 2 beta 1, rhOX40Ligand, rhTRAIL, rhTRANCE, rhTWEAK, rhVEGI, recombinant mouse (rm) 4-1BBLigand, rmBAFF, rmFas Ligand, rmGITR Ligand, rmLIGHT, rmOX40 Ligand, rmTRAIL,rmTRAIL-2L, rmTRANCE, or rmTWEAK is observed. |
| 使用方法 | Neutralization: Measured by its ability to neutralize TNF‑ alpha -induced cytotoxicity in the L‑929 mouse fibroblast cell line. Matthews, N. and M. L. Neale (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M. J. et al. (eds): IRL Press. 221. The Neutralization Dose (ND50) is typically 0.4-2.4 µg/mL in the presence of 0.25 ng/mL Recombinant Rabbit TNF‑ alpha and the metabolic inhibitor actinomycin D (1 µg/mL). |
| 来源 | Monoclonal Mouse IgG2A Clone # 610331 |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 7124 (Human); 21926 (Mouse); 24835 (Rat); 397086 (Porcine); 280943 (Bovine); 403922 (Canine); 100033834 (Equine); 493755 (Feline); 100009088 (Rabbit) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | E. coli-derived recombinant rabbit TNF-alpha Leu79-Leu234 Accession # NP_001075732 |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Rabbit |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | Tumor necrosis factor (TNF-alpha ), also known as cachectin and TNFSF2, is the prototypic ligand of the TNF superfamily and family. The 26 kDa type II transmembrane protein is assembled intracellularly to form a noncovalently linked homotrimer. Rabbit TNF-alpha is 235 amino acids (aa) in length and contains a 35 aa cytoplasmic domain, a 21 aa transmembrane region, and a 179 extracellular domain (ECD). Cleavage of membrane bound TNF-alpha by TACE/ADAM17 releases a 55 kDa soluble trimeric form of TNF-alpha. Rabbit TNF-alpha is 79% and 78% aa identical to human and mouse TNF-alpha, respectively. TNF-alpha is produced by several lymphoid cells as well as by astrocytes, endothelial cells, and smooth muscle cells. TNF-alpha binds TNF RI and TNF RII present on virtually all cell types where it triggers the activation of multiple signal transduction pathways and modulates the expression of a wide variety of genes. |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 |
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Cytotoxicity Induced by TNF‑ alpha and Neutralization by Rabbit TNF‑ alpha Antibody. Recombinant Rabbit TNF‑ alpha (Catalog # 5670-TG) induces cytotoxicity in the the L‑929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by Resazurin. Cytotoxicity elicited by Recombinant Rabbit TNF‑ alpha (0.25 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Rabbit TNF‑ alpha Monoclonal Antibody (Catalog # MAB5670). The ND50 is typically 0.4‑2.4 µg/mL in the presence of the metabolic inhibitor actinomycin D (1 µg/mL). |
