Human CXCL7/NAP-2 MAb (Clone 59418) (500 UG)【科瑞奥博】

产品中心

当前位置：首页>产品中心

Human CXCL7/NAP-2 MAb (Clone 59418) (500 UG)

货号： MAB393 基本售价： 5026.6 元规格： 500 ug

产品信息

概述
货号	MAB393
描述	For ELISA the Antibody Pairs information：Capture antibody：MAB393；Detection antibody：BAF393; and protein: 393-NP-010
别名	Beta-TG; connective tissue-activating peptide III; CTAP III; CTAP3CTAP-III; CTAPIII; CXC chemokine ligand 7; C-X-C motif chemokine 7; CXCL7b-TG1; LA-PF4; LDGFTC1; Leukocyte-derived growth factor; low-affinity platelet factor IV; Macrophage-derived growth factor; MDGFTC2; NAP-2; NAP-2-L1; neutrophil-activating peptide 2; neutrophil-activating peptide-2; PBPTHBGB; platelet basic protein; PPBP; pro-platelet basic protein (chemokine (C-X-C motif) ligand 7); SCAR10; SCYB7beta-thromboglobulin; small inducible cytokine B7; small inducible cytokine subfamily B, member 7; Small-inducible cytokine B7; TGB; TGB1B-TG1; THBGB1; thrombocidin 1; thrombocidin 2; thromboglobulin, beta-1
反应种属	Human
应用	Western Blot,Simple Western,ELISA Capture (Matched Antibody Pair),ELISA Detection (Matched Antibody Pair),ELISA Standard ,Neutralization
目标/特异性	Detects human CXCL7/NAP‑2 in ELISAs and Western blots. In sandwich ELISAs, less than 0.05% cross-reactivity with recombinant human (rh) CXCL1, 2, 3, 5, 6, 8, 9, 10, or 12 is observed. In Western blots, no cross-reactivity with rhCXCL1, 2, 3, 5, 6, 8, 9, 10, 12, 13, recombinant mouse CXCL1, 2, 6, 9, 10, 12 or recombinant rat CXCL1, 2 or 3 is observed.
使用方法	Western Blot: 1 µg/mL Simple Western: 10 µg/mL ELISA Capture (Matched Antibody Pair): 2-8 µg/mL ELISA Detection (Matched Antibody Pair): 0.1-0.4 µg/mL ELISA Standard : Neutralization: Measured by its ability to neutralize CXCL7/NAP‑2-induced myeloperoxidase release in cytochalasin‑B treated human neutrophils. Schröder, J. M. et al. (1987) J. Immunol. 139:3474. The Neutralization Dose (ND₅₀) is typically 15-20 µg/mL in the presence of 1 µg/mL Recombinant Human CXCL7/NAP‑2 and cytochalasin‑B.
来源	Monoclonal Mouse IgG₁ Clone # 59418
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	5473 (Human)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Up-regulation of platelet activation in hemophilia A. Authors: van Bladel ER, Roest M Haematologica, 2011;0(0):. Species: Human Sample Type: Plasma Application: ELISA Development High platelet reactivity is associated with myocardial infarction in premenopausal women: a population-based case-control study. Authors: Snoep JD, Roest M, Barendrecht AD J. Thromb. Haemost., 2010;8(5):906-13. Species: Human Sample Type: Plasma Application: ELISA Development Increased levels of neutrophil-activating peptide-2 in acute coronary syndromes: possible role of platelet-mediated vascular inflammation. Authors: Smith C, Damas JK, Otterdal K, ÃƒËœIe E, Sandberg WJ, Yndestad A, Waehre T, Scholz H, Endresen K, Olofsson PS, Halvorsen B, Gullestad L, Froland SS, Hansson GK, Aukrust P J. Am. Coll. Cardiol., 2006;48(8):1591-9. Species: Human Sample Type: Whole Cells Application: Neut
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	E. coli-derived recombinant human CXCL7/NAP-2 Ala59-Asp128 Accession # P02775
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	CXCL7, also known as NAP-2, is a 11 kDa angiogenic alpha -chemokine that is produced by the proteolytic cleavage of the inactive 13 kDa platelet basic protein (PBP). PBP, CXCL7, and C-terminally truncated forms of CXCL7 are secreted by monocytes and platelets. Active CXCL7 binds CXCR2 and induces the chemoattration of monocytes, neutrophils, and endothelial progenitor cells. It also inhibits megakaryocytopoiesis in the bone marrow. Within the region included in this immunogen, human CXCL7 shares 52% aa sequence identity with mouse and rat CXCL7.
运输条件	Blue Ice
存放说明	-20℃
参考文献	Chemokines and Receptors Chemokines and Receptors in Angiogenesis CXC Chemokines and Receptors

参考图片

Myeloperoxidase Release Induced by CXCL7/NAP‑2 and Neutralization by Human CXCL7/NAP‑2 Antibody. Recombinant Human CXCL7/NAP‑2 (Catalog #
393‑NP) induces myeloperoxidase release in the cytochalasin‑B treated human neutrophils in a dose-dependent manner (orange line). Myeloperoxidase Release elicited by Recombinant Human CXCL7/NAP‑2 (1 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CXCL7/
NAP‑2 Monoclonal Antibody (Catalog # MAB393). The ND₅₀ is typically 15‑20 µg/mL in the presence of cytochalasin‑B.

Detection of Human CXCL7/NAP‑2 by Simple Western^TM. Simple Western lane view shows lysates of human platelets, loaded at 0.2 mg/mL. A specific band was detected for CXCL7/NAP‑2 at approximately 10 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human CXCL7/NAP‑2 Monoclonal Antibody (Catalog # MAB393). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.