| 货号 | MAB3867 |
| 别名 | FGF-22; fibroblast growth factor 22 | 全称 | Fibroblast Growth Factor 22 |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects human FGF-22 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human (rh) FGF-basic, rhFGF-3, -4, -5, -6, -7, -9, -10, -11, -12, -13, -16, -17, -18, -19, -20, -21, -23, recombinant mouse (rm) FGF-basic, rmFGF-8C, or -15 is observed. |
| 使用方法 | Western Blot: 1 µg/mL Neutralization: Measured by its ability to neutralize FGF‑22-induced proliferation in the 4MBr‑5 rhesus monkey epithelial cell line. The Neutralization Dose (ND50) is typically 1.5-7.5 µg/mL in the presence of 1 µg/mL Recombinant Human FGF‑22. |
| 来源 | Monoclonal Mouse IgG2A Clone # 435008 |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 27006 (Human); 67112 (Mouse); 170579 (Rat) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | E. coli-derived recombinant human FGF-22 Thr23-Ser170 Accession # Q9HCT0 |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | Fibroblast growth factor-22 (FGF-22) is a 23 kDa, non-glycosylated member of the FGF-7 subfamily, from the FGF family of heparin-binding growth factors (1‑3). The human FGF-22 precursor is 170 amino acids (aa) in length, and contains a 22 aa signal sequence with a 148 aa mature region (4‑6). The mature region shows a centrally-placed, 120 aa beta -trefoil region (aa 43‑168) that is characteristic of all FGF family members. Human FGF-22 potentially has one alternate splice form. This isoform is 129 aa in length, and shows a 31 aa substitution for the first N-terminal 72 aa of the standard, or long, form (7). There is no information related to its possible function. Mature human FGF-22 is 86% aa identical to mouse FGF-22, with the mouse molecule showing a 9 aa deletion at the N-terminus (5). FGF-22 is synthesized by at least three cell types; keratinocytes, neurons, and skeletal muscle myotubes (4, 8, 9). In neurons and myotubes, FGF-22 is presumed to function as an organizer of the presynaptic apparatus. Expressed by postsynaptic (or target) cells, FGF-22 is believed to bind to FGF R2b on the surface of innervating processes, resulting in synaptic vesicle clustering, organization, and neurite branching (8, 10). Although FGF-22 is assumed to be secreted, little can be found in expressing cell culture media. Presumably, it is bound to 34 kDa FGF-BP1, which is a molecule described as typically associated with cell membrane proteoglycans (6, 11). Thus, following secretion, FGF-22 could quickly be immobilized by FGF-BP1, only to be released at a later time, or aided by FGF-BP1 in its interaction with FGF R2b (6, 10, 11). |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 |
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Cell Proliferation Induced by FGF‑22 and Neutralization by Human FGF‑22 Antibody. Recombinant Human FGF‑22 (Catalog # 3867-FG) stimulates proliferation in the 4MBr‑5 rhesus monkey epithelial cell line in a dose‑dependent manner (orange line). Proliferation elicited by Recombinant Human FGF‑22 (1 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human FGF‑22 Monoclonal Antibody (Catalog # MAB3867). The ND50 is typically 1.5‑7.5 µg/mL. |
