Rat IL-1 beta/IL-1F2 MAb (Clone 38139) (500 UG)【科瑞奥博】

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Rat IL-1 beta/IL-1F2 MAb (Clone 38139) (500 UG)

货号： MAB501 基本售价： 5026.6 元规格： 500 ug

产品信息

概述
货号	MAB501
别名	catabolin; IL-1 beta; IL-1; IL1B; IL-1B; IL1-BETA; IL-1F2; IL1F2IL-1 beta; interleukin 1, beta; interleukin-1 beta; preinterleukin 1 beta; pro-interleukin-1-beta
全称	Interleukin 1 beta
反应种属	Rat
应用	Immunocytochemistry(8-25 µg/mL)
目标/特异性	Detects rat IL‑1 beta /IL‑1F2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross‑reactivity with recombinant human IL-1 alpha, recombinant mouse IL-1 alpha, and recombinant rat IL-1 alpha is observed.
使用方法	Immunocytochemistry: 8-25 µg/mL Neutralization: Measured by its ability to neutralize IL‑1 beta /IL‑1F2-induced proliferation in the D10.G4.1 mouse helper T cell line. Symons, J. A. et al. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M. J. et al. (eds): IRL Press. 272. The Neutralization Dose (ND₅₀) is typically 10-30 µg/mL in the presence of 4 ng/mL Recombinant Rat IL‑1 beta /IL‑1F2 and 1.25 µg/mL concanavalin A.
来源	Monoclonal Mouse IgG₁ Clone # 38139
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	3553 (Human); 16176 (Mouse); 24494 (Rat); 397122 (Porcine); 403974 (Canine); 100034237 (Equine); 100135556 (Guinea Pig)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Ischemic neurons prevent vascular regeneration of neural tissue by secreting semaphorin 3A. Authors: Joyal JS, Sitaras N, Binet F, Rivera JC, Stahl A, Zaniolo K, Shao Z, Polosa A, Zhu T, Hamel D, Djavari M, Kunik D, Honore JC, Picard E, Zabeida A, Varma DR, Hickson G, Mancini J, Klagsbrun M, Costantino S, Beausejour C, Lachapelle P, Smith LE, Chemtob S, Sapieha P Blood, 2011;117(22):6024-35. Species: Mouse Sample Type: Tissue Homogenates Application: WB Cytokine production by mouse myeloid dendritic cells in relation to differentiation and terminal maturation induced by lipopolysaccharide or CD40 ligation. Authors: Morelli AE, Zahorchak AF, Larregina AT Blood, 2001;98(5):1512-23. Species: Mouse Sample Type: Whole Cells Application: Flow
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	E. coli-derived recombinant rat IL‑1 beta /IL‑1F2 Val117-Ser268 Accession # Q63264
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Rat
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	IL-1 beta is a prototypical member of the IL-1 superfamily and is designated IL-1F2. The biological activity of IL-1 beta is mediated by the heterodimeric receptor complex containing IL-1 R1(IL-1 RI) and IL-1 R3 (IL-1 RAcP). IL-1 beta is a pleiotropic cytokineinvolved in inflammation, development, growth and appetite.
运输条件	Blue Ice
存放说明	-20℃
参考文献	Cancer Biomarkers Chronic IL-1 beta-induced Neuroinflammation Is it Really that Bad? Conventional/Classical Dendritic Cells Cytokines and Receptors in Allergy and Asthma Cytokines and Receptors in Angiogenesis ICC / IHC Images: IL-1 beta/IL-1F2 IL-1 Family Infectious Diseases Inflammasomes: Intracellular Regulators of Pathogen Inflammatory Mediators Interactive Pathway: IL-1 Family Signaling Pathways Isolation, Culture, and Identification of Natural Killer Cells LTi Cells Macrophage Activation Markers Mini-review: Interleukin 1 Multiple Sclerosis and Myelin Related Molecules Myeloid-derived Suppressor Cells (MDSC) NCR-ILC3 Neuroinflammation NOD-like Receptors and the Inflammasome Platelets Regulatory T Cells (Tregs) T Cell Cytokine Signaling Th17 Cells Th9 Cells

参考图片

Cell Proliferation Induced by IL‑1 beta /IL‑1F2 and Neutralization by Rat IL‑1 beta /IL‑1F2 Antibody. Recombinant Rat IL‑1 beta /IL‑1F2 (Catalog # 501-RL) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Rat IL‑1 beta /IL‑1F2 (4 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Rat IL‑1 beta /IL‑1F2 Monoclonal Antibody (Catalog # MAB501). The ND₅₀ is typically 10-30 µg/mL in the presence of concanavalin A (1.25 µg/mL).