Porcine IL-10 MAb (Clone 148801) (500 UG)【科瑞奥博】

产品中心

当前位置：首页>产品中心

Porcine IL-10 MAb (Clone 148801) (500 UG)

货号： MAB6931 基本售价： 5026.6 元规格： 500 ug

产品信息

概述
货号	MAB6931
别名	CSIF; CSIFMGC126450; Cytokine synthesis inhibitory factor; IL10A; IL-10MGC126451; interleukin 10; interleukin-10; TGIF
全称	Interleukin 10
反应种属	Porcine
应用	Neutralization
目标/特异性	Detects porcine IL‑10 in direct ELISAs. In direct ELISAs, 25-50% cross-reactivity withrecombinant feline IL-10, recombinant canine IL-10, and recombinant guinea pigIL-10 is observed, approximately 10% cross-reactivity with recombinant humanIL-10 is observed, less than 5% cross-reactivity with recombinant cotton ratIL-10 is observed, and no cross-reactivity with recombinant equine IL-10,recombinant mouse IL-10, recombinant rat IL-10, or recombinant viral IL-10 isobserved.
使用方法	Neutralization: Measured by its ability to neutralize IL‑10-induced proliferation in the MC/9‑2 mouse mast cell line. Thompson-Snipes, L. et al. (1991) J. Exp. Med. 173:507. The Neutralization Dose (ND₅₀) is typically 0.05-0.15 µg/mL in the presence of 40 ng/mL Recombinant Porcine IL‑10.
来源	Monoclonal Mouse IgG_2B Clone # 148801
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	3586 (Human); 16153 (Mouse); 25325 (Rat); 397106 (Porcine); 403628 (Canine); 493683 (Feline); 100135553 (Guinea Pig); 2949786 (Viral)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Differential Effects of Escherichia coli Nissle and Lactobacillus rhamnosus Strain GG on Human Rotavirus Binding, Infection, and B Cell Immunity. Authors: Kandasamy S, Vlasova A, Fischer D, Kumar A, Chattha K, Rauf A, Shao L, Langel S, Rajashekara G, Saif L J Immunol, 2016;196(4):1780-9. Species: Porcine Sample Type: Whole Cells Application: Neut Postnatal persistent infection with classical Swine Fever virus and its immunological implications. Authors: Munoz-Gonzalez S, Ruggli N, Rosell R, Perez L, Frias-Leuporeau M, Fraile L, Montoya M, Cordoba L, Domingo M, Ehrensperger F, Summerfield A, Ganges L PLoS ONE, 2015;10(5):e0125692. Species: Porcine Sample Type: Whole Cells Application: Neut The effect of activated protein C on plasma cytokine levels in a porcine model of acute endotoxemia. Authors: Nielsen JS, Larsson A, Rix T, Nyboe R, Gjedsted J, Krog J, Ledet T, Tonnesen E Intensive Care Med, 2007;33(6):1085-93. Species: Human Sample Type: Plasma Application: ELISA Development
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	E. coli-derived recombinant porcine IL-10 Ser19-Asn175 Accession # Q29055
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Porcine
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	IL-10, initially designated cytokine synthesis inhibitory factor (CSIF), was originally identified as a product of murine T helper 2 (Th2) clones that inhibited the cytokine production by Th1 clones which are dependent upon stimulation with antigen in the presence of antigen presenting cells (APC). Murine IL-10 is produced by Th2 cells, activated fetal thymocytes, macrophages, keratinocytes, and LY-1⁺(CD5⁺) and normal B cells. Human IL-10 has cross-species actvities and is active on mouse cells. Murine IL-10 is species-specific and does not act on human cells. Porcine IL-10 shares 71% and 78% amino acid sequence identitywith mouse and human IL-10, respectively. IL-10 is a pleiotropic cytokine that can exert either immunostimulatory or immunosuppressive effects on a variety of cell types. It is a potent immunosuppressant of macrophage functions. In vitro, IL-10 can inhibit the accessory function and antigen-presenting capacity of monocytes by, among other effects, down‑regulating class II MHC expression. Thus, IL-10 can inhibit monocyte/macrophage-dependent, antigen stimulated cytokine synthesis (especially IFN-gamma ) by human PBMNC and NK, and mouse Th1 cells. Additionally, IL-10 is a potent inhibitor of monocyte/macrophage activation and its resultant cytotoxic effects. As an immunostimulatory cytokine, IL-10 can act on B cells to enhance their viability, cell proliferation, Ig secretion, and class II MHC expression. Aside from B lymphocytes, IL-10 is also a growth co-stimulator for thymocytes and mast cells, as well as an enhancer of cytotoxic T cell development.
运输条件	Blue Ice
存放说明	-20℃
参考文献	Moore, K.W. et al. (1993) Annu. Rev. Immunol. 11:165.

参考图片

Cell Proliferation Induced by IL‑10 and Neutralization by Porcine IL‑10 Antibody. Recombinant Porcine IL‑10 (Catalog # 693-PI) stimulates proliferation in the MC/9‑2 mouse mast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Porcine IL‑10 (40 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti‑Porcine IL‑10 Monoclonal Antibody (Catalog # MAB6931). The ND₅₀ is typically 0.05‑0.15 µg/mL.