Human DC-SIGNR/CD299 MAb (Clone 120604) (500 UG)【科瑞奥博】

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Human DC-SIGNR/CD299 MAb (Clone 120604) (500 UG)

货号： MAB162 基本售价： 5026.6 元规格： 500 ug

产品信息

概述
货号	MAB162
简介	Clone 120604 was used by HLDA to establish CD designation
别名	CD209L; CD209L1; CD209Ldendritic cell-specific ICAM-3-grabbing nonintegrin 2; CD299 antigenMGC129964; CD299; CLEC4M; C-type lectin domain family 4, member M; DC-SIGN2; DC-SIGN2CD209 antigen-like protein 1; DC-SIGNRDC-SIGN-related protein; DCSIGNRMGC47866; Dendritic cell-specific ICAM-3-grabbing non-integrin 2; HP10347; liver/lymph node-specific ICAM-3 grabbing non-integrin; Liver/lymph node-specific ICAM-3-grabbing non-integrin; LSIGN; L-SIGN; LSIGNC-type lectin domain family 4 member M; mannose binding C-type lectin DC-SIGNR
全称	Dendritic Cell-specific ICAM-3-grabbing Non-integrin 2
反应种属	Human
应用	Flow Cytometry,Adhesion Blockade,CyTOF-ready
目标/特异性	Detects human DC‑SIGNR/CD299.
使用方法	Flow Cytometry: 2.5 µg/10⁶cells Adhesion Blockade: The adhesion of NIH-3T3 mouse embryonic fibroblast cells (5 x 10⁴ cells/well) to immobilized Recombinant Human ICAM-3/CD50 Fc Chimera (Catalog # 715-IC, 5 µg/mL, 100 µL/well) was maximally inhibited (80-100%) by 2.5 µg/mL of the antibody.
来源	Monoclonal Mouse IgG_2B Clone # 120604
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	10332 (Human)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Respiratory syncytial virus glycoprotein G interacts with DC-SIGN and L-SIGN to activate ERK1 and ERK2. Authors: Johnson TR, McLellan JS, Graham BS J. Virol., 2012;86(3):1339-47. Species: Human Sample Type: Whole Cells Application: Neut DC-SIGN mediates cell-free infection and transmission of human T-cell lymphotropic virus type 1 by dendritic cells. Authors: Jain P, Manuel SL, Khan ZK, Ahuja J, Quann K, Wigdahl B J. Virol., 2009;83(21):10908-21. Species: Human Sample Type: Whole Cells Application: Neut Adhesion of human haematopoietic (CD34+) stem cells to human liver compartments is integrin and CD44 dependent and modulated by CXCR3 and CXCR4. Authors: Crosby HA, Lalor PF, Ross E, Newsome PN, Adams DH J. Hepatol., 2009;51(4):734-49. Species: Human Sample Type: Whole Tissue Application: IHC Human liver sinusoidal endothelial cells induce apoptosis in activated T cells: a role in tolerance induction. Authors: Karrar A, Broome U, Uzunel M, Qureshi AR, Sumitran-Holgersson S Gut, 2006;56(2):243-52. Species: Human Sample Type: Whole Tissue Application: IHC Frozen Expression of DC-SIGN and DC-SIGNR on human sinusoidal endothelium: a role for capturing hepatitis C virus particles. Authors: Lai WK, Sun PJ, Zhang J, Jennings A, Lalor PF, Hubscher S, McKeating JA, Adams DH Am. J. Pathol., 2006;169(1):200-8. Species: Human Sample Type: Whole Tissue Application: IHC DC-SIGN and CLEC-2 mediate human immunodeficiency virus type 1 capture by platelets. Authors: Chaipan C, Soilleux EJ, Simpson P, Hofmann H, Gramberg T, Marzi A, Geier M, Stewart EA, Eisemann J, Steinkasserer A, Suzuki-Inoue K, Fuller GL, Pearce AC, Watson SP, Hoxie JA, Baribaud F, Pohlmann S J. Virol., 2006;80(18):8951-60. Species: Human Sample Type: Whole Cells Application: Neut Hepatitis C virus targets DC-SIGN and L-SIGN to escape lysosomal degradation. Authors: Ludwig IS, Lekkerkerker AN, Depla E, Bosman F, Musters RJ, Depraetere S, van Kooyk Y, Geijtenbeek TB J. Virol., 2004;78(15):8322-32. Species: Human Sample Type: Whole Cells Application: ICC
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	NIH-3T3 mouse embryonic fibroblast cell line transfected with human DC‑SIGNR/CD299 Accession # Q9H2X3
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	Dendritic cell-specific ICAM-3 grabbing non-integrin (DC-SIGN or CD299) and DC-SIGN related protein (DC-SIGNR, DC-SIGN2, L-SIGN or CD209L) are type II membrane proteins that are mannose-specific calcium-dependent (C-type) lectins. The two proteins share 77% amino acid identity. DC-SIGN mediates interactions between dendritic cells (DCs) and T cells. Both DC-SIGN and DC-SIGNR have been shown to bind HIV, hepatitis C glycoproteins, Ebola virus glycoproteins and the cellular adhesion protein ICAM-3 (1‑4). DC-SIGN and DC-SIGNR appear to selectively recognize and bind viral proteins containing a large portion of high-mannose oligosaccharides (5). Though DC-SIGN and DC-SIGNR are found on the same chromosome, they are not expressed in the same tissue. DC-SIGN is expressed solely on Dendritic cells while DC-SIGNR is found on endothelial cells in the liver and lymph node sinuses and in a significant portion of capillary endothelial cells in term placenta (1, 4).
运输条件	Blue Ice
存放说明	-20℃
参考文献	Pohlmann, S. et al. (2001) Proc. Natl. Acad. Sci. USA 98:2670. Pohlmann, S. et al. (2003) J. Virol. 77:4070. Simmons, L.G. et al. (2003) J. Virol. 77:1337. Bahirova, A.A. et al. (2001) J. Exp. Med. 193:671. Feinberg, H. et al. (2001) Science 294:2163.

参考图片

Detection of DC‑SIGNR/CD299 in Human DC‑SIGNR/CD299 Transfected 3T3 Mouse Cell Line by Flow Cytometry.

Human DC‑SIGNR/CD299 and DC‑SIGN transfected 3T3 mouse embryonic fibroblast cell line were stained with Mouse Anti-Human DC‑SIGNR/CD299 Monoclonal Antibody (Catalog # MAB162, filled histograms) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab)₂ Secondary Antibody (Catalog # F0102B).