Human FGF-21 MAb (Clone 40020A) ( )【科瑞奥博】

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Human FGF-21 MAb (Clone 40020A) ( )

货号： MAB25373-100 基本售价： 3985.6 元规格： 100 ug

产品信息

概述
货号	MAB25373-100
简介	Recombinant Monoclonal Antibody
别名	FGF21; FGF-21; fibroblast growth factor 21
全称	Fibroblast Growth Factor 21
应用	Neutralization
目标/特异性	Detects human FGF-21 in direct ELISAs.
使用方法	Neutralization: Measured by its ability to neutralize FGF-21-induced proliferation in BaF3 mouse pro-B cell line transfected with human FGF-RIIIc. The Neutralization Dose (ND₅₀) is typically 0.5-3 μg/mL in the presence of Recombinant Mouse Klotho beta.
来源	Recombinant Monoclonal Goat IgG_2B Clone # 40020A
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	26291 (Human); 56636 (Mouse)
纯化方式	Protein A or G purified from cell culture supernatant
免疫原	E. coli-derived recombinant human FGF-21 His29-Ser209 Accession # Q9NSA1
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitue at 0.5mg/mL in sterile PBS.
背景	Fibroblast growth factor 21 (FGF-21) is a member of the FGF gene family, which currently contains 22 human members. Based on its structure, it is further classified as an FGF19 subfamily member. This subfamily includes FGF-19, -21, and -23. Like all other FGF subfamilies, FGF-19 subfamily members contain a 120 amino acid (aa) core FGF domain that exhibits a beta -trefoil structure (1, 2). Unlike other FGF subfamilies, FGF-19 subfamily members apparently exhibit poor binding to ECM, resulting in highly diffusible molecules (3). The c-DNA for FGF-21 predicts a 209 aa polypeptide that contains a 28 aa signal sequence and a 181 aa mature region (4). Notably, FGF-21, as well as FGF-19 show limited binding to heparin (4). One potential alternate splice form has been reported. It shows a 43 aa substitution for the C-terminal 12 aa of the standard form (5). Mature human FGF-21 shows 81% aa identity to mouse FGF-21, and is known to be active on mouse cells (4, 6). The FGF-19 subfamily is considered endocrine in nature. All three subfamily members impact some aspect of metabolism, all three are induced by a nuclear receptor heterodimer that includes RXR, and all three utilize Klotho family members for signal transduction (7, 8, 9). FGF-21 is produced by hepatocytes in response to free fatty acid (FFA) stimulation of a PPARa/RXR dimeric complex (3, 7, 10, 11). This situation occurs clinically during starvation, or following the ingestion of a high-fat/low-carbohydrate diet. Upon FGF-21 secretion, white adipose tissue is induced to release FFAs from triglyceride stores. Once FFAs reach hepatocytes, they are oxidized and reduced to acetyl-CoA. The acetyl-CoA is recombined into 4-carbon ketone bodies (acetoacetate and beta -hydroxybutyrate), released, and transported to peripheral tissues for TCA processing and energy generation (11, 12).
运输条件	Blue Ice
存放说明	-20℃
参考文献	Itoh, N. and D.M. Ornitz (2004) Trends Genet. 20:563. Mohammadi, M. et al. (2005) Cytokine Growth Factor Rev. 16:107. Huang, X. et al. (2006) Mol. Carcinog. 45:934. Nishimura, T. et al. (2000) Biochim. Biophys. Acta 1492:203. GenBank Accession #: EAW52401 (2006). Ford, A.M. et al. (2005) J. Clin. Invest. 115:1627. Moore, D. D. (2007) Science 316:1436. Ogawa, Y. et al. (2007) Proc. Natl. Acad. Sci. USA 104:7432. Kurosu, H. et. al. (2007) J. Biol. Chem. 282:26687. Lundasen, T. et al. (2007) Biochem. Biophys. Res. Commun. 360:437. Badman, M.K. et al. (2007) Cell Metab. 5:426. Inagaki, T. et al. (2007) Cell Metab. 5:415.

参考图片

Cell Proliferation Induced by FGF-21 and Neutralization by Human FGF-21 Antibody. In the presence of Recombinant Mouse Klotho beta (Catalog # 2619-KB), Recombinant Human FGF-21 (Catalog # 2539-FG) stimulates proliferation in the BaF3 mouse pro-B cell line transfected with human FGF-RIIIc in a dose-dependent manner (orange line). Under these conditions, proliferation elicited by Recombinant Human FGF-21 (500 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human FGF-21 Mononclonal Antibody (MAB25373). The ND₅₀ is typically 0.5-3 μg/mL.