Human LAP (TGF-beta 1) MAb (Clone 27235) (100 UG)【科瑞奥博】

产品中心

当前位置：首页>产品中心

Human LAP (TGF-beta 1) MAb (Clone 27235) (100 UG)

货号： MAB246-100 基本售价： 3985.6 元规格： 100 ug

产品信息

概述
货号	MAB246-100
别名	LAP (TGFbeta 1); LAP (TGF-beta 1)
全称	Latency-associated Peptide
反应种属	Human
应用	Immunocytochemistry,Neutralization
目标/特异性	Detects human LAP in direct ELISAs and Western blots.
使用方法	Neutralization: Measured by its ability to neutralize LAP TGF‑ beta 1 inhibition of TGF‑ beta 1 growth inhibition in the HT‑2 mouse T cell line. Tsang, M. et al. (1995) Cytokine 7:389. The Neutralization Dose (ND₅₀) is typically 1-5 µg/mL in the presence of 500 ng/mL Recombinant Human LAP TGF‑ beta 1 and 1 ng/mL Recombinant Human TGF‑ beta 1.
来源	Monoclonal Mouse IgG₁ Clone # 27235
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	7040 (Human)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Induction of latency-associated peptide (transforming growth factor-beta(1)) expression on CD4+ T cells reduces Toll-like receptor 4 ligand-induced tumour necrosis factor-alpha production in a transforming growth factor-beta-dependent manner. Authors: Boswell S, Sharif S, Alisa A, Pereira SP, Williams R, Behboudi S Immunology, 2011;133(3):278-87. Species: Human Sample Type: Whole Cells Application: Neut Cord blood CD4(+)CD25(+)-derived T regulatory cell lines express FoxP3 protein and manifest potent suppressor function. Authors: Godfrey WR, Spoden DJ, Ge YG, Baker SR, Liu B, Levine BL, June CH, Blazar BR, Porter SB Blood, 2005;105(2):750-8. Species: Human Sample Type: Cell Culture Supernates Application: Neut Suppressive properties of human CD4+CD25+ regulatory T cells are dependent on CTLA-4 expression. Authors: Birebent B, Lorho R, Lechartier H, de Guibert S, Alizadeh M, Vu N, Beauplet A, Robillard N, Semana G Eur. J. Immunol., 2004;34(12):3485-96. Species: Human Sample Type: Whole Cells Application: Flow
纯化方式	Protein A or G purified from ascites
免疫原	S. frugiperda insect ovarian cell line Sf 21-derived recombinant human LAP (TGF-beta 1)
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	The TGF-beta family includes several related proteins (70-80% sequence homology) from mammalian, avian, or Xenopussystems that are now designated TGF-beta 1, TGF‑ beta 2, TGF-beta 1.2, TGF-beta 3, TGF-beta 4, and TGF-beta 5. These proteins are secreted by cells in the form of an inactive complex, referred to as latent TGF-beta, that consists of TGF-beta associated non-covalently with a Latency-associated peptide (LAP). These two proteins are synthesized as a single pro-peptide that is cleaved in a post Golgi compartment prior to secretion. Different TGF-beta family members are naturally associated with their own distinct LAPs.
运输条件	Blue Ice
存放说明	-20℃
参考文献	Airway Inflammation and Remodeling Cytokines and Receptors in Angiogenesis gamma delta T Cells Interactive Pathway: TGF-beta Signaling Pathways Multiple Sclerosis and Myelin Related Molecules Myeloid-derived Suppressor Cells (MDSC) T Cell Cytokine Signaling TGF-beta Family TGF-beta Family Ligands Th17 Cells Th22 Cells Th9 Cells

参考图片

LAP TGF‑ beta 1 Inhibition of TGF‑ beta 1 Activity and Neutralization by Human LAP TGF‑ beta 1 Antibody. Recombinant Human LAP TGF‑ beta 1 (Catalog # 246-LP) inhibits Recombinant Human TGF‑ beta 1 (Catalog # 240-B) growth inhibition activity in the HT‑2 mouse T cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human TGF‑ beta 1 (1 ng/mL) activity elicited by Recombinant Human LAP TGF‑ beta 1 (500 ng/mL) is neutralized (green line) by increasing concentrations of Human LAP TGF‑ beta 1 Monoclonal Antibody (Catalog # MAB246). The ND₅₀ is typically 1-5 µg/mL.