Human Oncostatin M (OSM) MAb (Clone 17001) (100 UG)【科瑞奥博】

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Human Oncostatin M (OSM) MAb (Clone 17001) (100 UG)

货号： MAB295-100 基本售价： 3985.6 元规格： 100 ug

产品信息

概述
货号	MAB295-100
别名	MGC20461; oncostatin M; oncostatin-M
反应种属	Human
应用	Western Blot(1 µg/mL) ELISA Capture (Matched Antibody Pair)(2-8 µg/mL ) ELISA Detection (Matched Antibody Pair)(0.1-0.4 µg/mL ) ELISA Standard ( )
目标/特异性	Detects human Oncostatin M in ELISAs and Western blots. In ELISAs, this antibody does not cross-react with recombinant human (rh) IL-6, rhIL-11, rhCNTF, or rhLIF.
使用方法	Western Blot: 1 µg/mL ELISA Capture (Matched Antibody Pair): 2-8 µg/mL ELISA Detection (Matched Antibody Pair): 0.1-0.4 µg/mL ELISA Standard : Neutralization: Measured by its ability to neutralize Oncostatin M/OSM-induced proliferation in the TF‑1 human erythroleukemic cell line. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The Neutralization Dose (ND₅₀) is typically 0.5-2.0 µg/mL in the presence of 2 ng/mL Recombinant Human Oncostatin M/OSM.
来源	Monoclonal Mouse IgG_2A Clone # 17001
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	5008 (Human); 18413 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Osteopontin Promotes Oncostatin M Production in Human Osteoblasts: Implication of Rheumatoid Arthritis Therapy. Authors: Su C, Chiang Y, Huang C, Hsu C, Fong Y, Tang C J Immunol, 2015;195(7):3355-64. Species: Human Sample Type: Whole Cells Application: Neut Monocytes Induce STAT3 Activation in Human Mesenchymal Stem Cells to Promote Osteoblast Formation. Authors: Nicolaidou V, Wong MM, Redpath AN PLoS ONE, 2012;7(7):e39871. Species: Human Sample Type: Whole Cells Application: Neut Microarray and Proteomic Analysis of Breast Cancer Cell and Osteoblast Co-cultures: ROLE OF OSTEOBLAST MATRIX METALLOPROTEINASE (MMP)-13 IN BONE METASTASIS. Authors: Morrison C, Mancini S, Cipollone J, Kappelhoff R, Roskelley C, Overall C J. Biol. Chem., 2011;286(39):34271-85. Species: Human Sample Type: Cell Culture Supernates Application: WB Tumor-associated leukemia inhibitory factor and IL-6 skew monocyte differentiation into tumor-associated macrophage-like cells. Authors: Duluc D, Delneste Y, Tan F, Moles MP, Grimaud L, Lenoir J, Preisser L, Anegon I, Catala L, Ifrah N, Descamps P, Gamelin E, Gascan H, Hebbar M, Jeannin P Blood, 2007;110(13):4319-30. Species: Human Sample Type: Cell Culture Supernates Application: Neut Secretion of oncostatin M by infiltrating neutrophils: regulation of IL-6 and chemokine expression in human mesothelial cells. Authors: Hurst SM, McLoughlin RM, Monslow J, Owens S, Morgan L, Fuller GM, Topley N, Jones SA J. Immunol., 2002;169(9):5244-51. Species: Human Sample Type: Cell Culture Supernates Application: ELISA Development
纯化方式	Protein A or G purified from ascites
免疫原	E. coli-derived recombinant human Oncostatin M/OSM Ala26-Arg234 Accession # P13725
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	OSM is a cytokine originally isolated from medium conditioned by PMA-treated U-937 human histiocytic leukemia cells based on its ability to inhibit growth of A375 melanoma cells. The human OSM cDNA encodes a 252 amino acid pre-pro-OSM polypeptide with a 25 residue hydrophobic signal peptide and a hydrophilic C-terminal domain that are proteolytically processed to generate the 196 residue mature form of OSM. Although both mature and pro-OSM are equally active in radio-receptor assays, the mature OSM is 5- to 60-fold more active in growth inhibition assays. Thus, proteolytic processing of the pro-OSM peptide may be important in regulating the in vivo activities of OSM. OSM is a pleiotropic cytokine that initiates its biological activities by binding to specific cell surface receptors. The gp130, a signal transducing component ( beta subunit) of the IL-6, LIF and CNTF receptor complexes, was identified as a low-affinity OSM receptor that does not transduce OSM signals. The low affinity LIF receptor (LIF R, a gp130-related protein) has now been identified to be a component of a high-affinity OSM receptor that will transduce OSM signals. Since OSM is also active on cells that do not express LIF R, a specific OSM receptor that does not involve LIF R must also exist. Besides its growth inhibitory activities on human A375 melanoma and mouse M1 myeloid leukemic cells, as well as on other solid tumor cells, OSM also has growth stimulatory activities on normal fibroblasts, AIDS-Kaposi’s sarcoma cells, and a human erythroleukemia cell line, TF-1. Other OSM-mediated activities reported to date include: stimulation of plasminogen activator activity in cultured bovine aortic endothelial cells; regulation of IL-6 expression in human endothelial cells; and stimulation of LDL uptake and up-regulation of cell surface LDL receptors in HepG2 cells.
运输条件	Blue Ice
存放说明	-20℃
参考文献	Cytokines and Receptors in Allergy and Asthma Cytokines and Receptors in Angiogenesis Diabetes Hepatic Endoderm Human Oncostatin M and Mouse LIF Support Mouse ES Cell Pluripotency & Germline Competency ICC / IHC Images: Oncostatin M/OSM IL-6 Family Obesity OSM Induction of Chondrocyte MMP and TIMP-3 Gene Expression Pancreatic Endoderm

参考图片

Cell Proliferation Induced by Oncostatin M/OSM and Neutralization by Human Oncostatin M/OSM Antibody. Recombinant Human Oncostatin M/OSM (Catalog # 295-OM) stimulates proliferation in the TF‑1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human Oncostatin M/OSM (2 ng/mL) is neutralized (green line) by increasing concentrations of Human Oncostatin M/OSM Monoclonal Antibody (Catalog # MAB295). The ND₅₀ is typically 0.5-2.0 µg/mL.