Human CXCR4 (Fusin) MAb (Clone 44716) (100 UG)【科瑞奥博】

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Human CXCR4 (Fusin) MAb (Clone 44716) (100 UG)

货号： MAB172-100 基本售价： 3985.6 元规格： 100 ug

产品信息

概述
货号	MAB172-100
别名	CD184; chemokine (C-X-C motif) receptor 4; chemokine (C-X-C motif), receptor 4 (fusin); C-X-C chemokine receptor type 4; CXC-R4; CXCR-4; D2S201ESDF-1 receptor; FB22; Fusin; HM89NPYRL; HSY3RR; LAP3; LCR1; LESTRCD184 antigen; Leukocyte-derived seven transmembrane domain receptor; leukocyte-derived seven-transmembrane-domain receptor; lipopolysaccharide-associated protein 3; neuropeptide Y receptor Y3; NPY3RWHIM; NPYR; NPYY3R; seven transmembrane helix receptor; seven-transmembrane-segment receptor, spleen; Stromal cell-derived factor 1 receptor
反应种属	Human
应用	Flow Cytometry(0.25 µg/10⁶cells) Immunohistochemistry(8-25 µg/mL)
目标/特异性	Detects human CXCR4. It will also react with cells expressing feline CXCR4 but not rat CXCR4.
使用方法	Flow Cytometry: 0.25 µg/10⁶cells Immunohistochemistry: 8-25 µg/mL Neutralization: Measured by its ability to neutralize CXCL12/SDF‑1 alpha -induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR4. The Neutralization Dose (ND₅₀) is typically 2.5-12 µg/mL in the presence of 1 ng/mL Recombinant Human/Feline/Rhesus Macaque CXCL12/SDF‑1 alpha.
来源	Monoclonal Mouse IgG_2B Clone # 44716
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	7852 (Human); 12767 (Mouse); 60628 (Rat); 483900 (Canine); 493676 (Feline)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Signal transmission through the CXC chemokine receptor 4 (CXCR4) transmembrane helices Proc Natl Acad Sci USA, 2016;0(0):. Species: Human Sample Type: Whole Cells Application: IHC Fresh Expression of the chemokine receptor CXCR7 in CXCR4-expressing human 143B osteosarcoma cells enhances lung metastasis of intratibial xenografts in SCID mice. Authors: Brennecke P, Arlt M, Muff R, Campanile C, Gvozdenovic A, Husmann K, Holzwarth N, Cameroni E, Ehrensperger F, Thelen M, Born W, Fuchs B PLoS ONE, 2013;8(9):e74045. Species: Human Sample Type: tissue Application: IHC Paraffin-embedded PCaAnalyser: a 2D-image analysis based module for effective determination of prostate cancer progression in 3D culture. Authors: Hoque, Md Tamji, Windus, Louisa C, Lovitt, Carrie J, Avery, Vicky M PLoS ONE, 2013;8(11):e79865. Species: Human Sample Type: cells Application: ICC CXC chemokine receptor 4 is essential for maintenance of renal cell carcinoma-initiating cells and predicts metastasis. Authors: Gassenmaier M, Chen D, Buchner A, Henkel L, Schiemann M, Mack B, Schendel D, Zimmermann W, Pohla H Stem Cells, 2013;31(8):1467-76. Species: Human Sample Type: whole cells Application: IHC Paraffin-embedded The EGFR ligands amphiregulin and heparin-binding egf-like growth factor promote peritoneal carcinomatosis in CXCR4-expressing gastric cancer. Authors: Yasumoto K, Yamada T, Kawashima A, Wang W, Li Q, Donev IS, Tacheuchi S, Mouri H, Yamashita K, Ohtsubo K, Yano S Clin. Cancer Res., 2011;17(11):3619-30. Species: Human Sample Type: Whole Tissue Application: IHC Paraffin-embedded Intratumoral induction of CD103 triggers tumor-specific CTL function and CCR5-dependent T-cell retention. Authors: Franciszkiewicz K, Le Floch A, Jalil A, Vigant F, Robert T, Vergnon I, Mackiewicz A, Benihoud K, Validire P, Chouaib S, Combadiere C, Mami-Chouaib F Cancer Res., 2009;69(15):6249-55. Species: Human Sample Type: Whole Cells Application: Flow Clinical and biological significance of CXCL12 and CXCR4 expression in adult testes and germ cell tumours of adults and adolescents. Authors: Gilbert DC, Chandler I, McIntyre A, Goddard NC, Gabe R, Huddart RA, Shipley J J. Pathol., 2009;217(1):94-102. Species: Human Sample Type: Whole Cells Application: ICC A late requirement for Wnt and FGF signaling during activin-induced formation of foregut endoderm from mouse embryonic stem cells. Authors: Hansson M, Olesen DR, Peterslund JM, Engberg N, Kahn M, Winzi M, Klein T, Maddox-Hyttel P, Serup P Dev. Biol., 2009;330(2):286-304. Species: Mouse Sample Type: Whole Cells Application: Flow Alternative implication of CXCR4 in JAK2/STAT3 activation in small cell lung cancer. Authors: Pfeiffer M, Hartmann TN, Leick M, Catusse J, Schmitt-Graeff A, Burger M Br. J. Cancer, 2009;100(12):1949-56. Species: Human Sample Type: Cell Lysates Application: IP Regulation of CXCR4 by the Notch ligand delta-like 4 in endothelial cells. Authors: Williams CK, Segarra M, Sierra Mde L, Sainson RC, Tosato G, Harris AL Cancer Res., 2008;68(6):1889-95. Species: Human Sample Type: Whole Cells Application: Neut Dynamic stromal-epithelial interactions during progression of MCF10DCIS.com xenografts. Authors: Tait LR, Pauley RJ, Santner SJ, Heppner GH, Heng HH, Rak JW, Miller FR Int. J. Cancer, 2007;120(10):2127-34. Species: Human Sample Type: Whole Cells Application: ICC Importance of recruitment of bone marrow-derived CXCR4+ cells in post-infarct cardiac repair mediated by G-CSF. Authors: Misao Y, Takemura G, Arai M, Ohno T, Onogi H, Takahashi T, Minatoguchi S, Fujiwara T, Fujiwara H Cardiovasc. Res., 2006;71(3):455-65. Species: Rabbit Sample Type: Whole Tissue Application: IHC Paraffin-embedded Human melanoma metastases express functional CXCR4. Authors: Scala S, Giuliano P, Ascierto PA, Ierano C, Franco R, Napolitano M, Ottaiano A, Lombardi ML, Luongo M, Simeone E, Castiglia D, Mauro F, De Michele I, Calemma R, Botti G, Caraco C, Nicoletti G, Satriano RA, Castello G Clin. Cancer Res., 2006;12(8):2427-33. Species: Human Sample Type: Whole Cells Application: ICC Chemokine and chemokine receptor expression in paired peripheral blood mononuclear cells and synovial tissue of patients with rheumatoid arthritis, osteoarthritis, and reactive arthritis. Authors: Haringman JJ, Smeets TJ, Reinders-Blankert P Ann. Rheum. Dis., 2006;65(3):294-300. Species: Human Sample Type: Whole Tissue Application: IHC Frozen Cutting edge: nonproliferating mature immune cells form a novel type of organized lymphoid structure in idiopathic pulmonary fibrosis. Authors: Marchal-Somme J, Uzunhan Y, Marchand-Adam S, Valeyre D, Soumelis V, Crestani B, Soler P J. Immunol., 2006;176(10):5735-9. Species: Human Sample Type: Whole Tissue Application: IHC Frozen Recruitment of osteoclast precursors by stromal cell derived factor-1 (SDF-1) in giant cell tumor of bone. Authors: Liao TS, Yurgelun MB, Chang SS, Zhang HZ, Murakami K, Blaine TA, Parisien MV, Kim W, Winchester RJ, Lee FY J. Orthop. Res., 2005;23(1):203-9. Species: Human Sample Type: Whole Cells Application: ICC Ultraviolet radiation-induced injury, chemokines, and leukocyte recruitment: An amplification cycle triggering cutaneous lupus erythematosus. Authors: Meller S, Winterberg F, Gilliet M, Muller A, Lauceviciute I, Rieker J, Neumann NJ, Kubitza R, Gombert M, Bunemann E, Wiesner U, Franken-Kunkel P, Kanzler H, Dieu-Nosjean MC, Amara A, Ruzicka T, Lehmann P, Zlotnik A, Homey B Arthritis Rheum., 2005;52(5):1504-16. Species: Human Sample Type: Whole Tissue Application: IHC Fresh/Frozen Intracellular localization and constitutive endocytosis of CXCR4 in human CD34+ hematopoietic progenitor cells. Authors: Zhang Y, Foudi A, Geay JF, Berthebaud M, Buet D, Jarrier P, Jalil A, Vainchenker W, Louache F Stem Cells, 2004;22(6):1015-29. Species: Human Sample Type: Whole Cells Application: ICC CXCR4 antagonist inhibits stromal cell-derived factor 1-induced migration and invasion of human pancreatic cancer. Authors: Mori T, Doi R, Koizumi M, Toyoda E, Ito D, Kami K, Masui T, Fujimoto K, Tamamura H, Hiramatsu K, Fujii N, Imamura M Mol. Cancer Ther., 2004;3(1):29-37. Species: Human Sample Type: Whole Cells Application: ICC CXCR4 enhances adhesion of B16 tumor cells to endothelial cells in vitro and in vivo via beta(1) integrin. Authors: Cardones AR, Murakami T, Hwang ST Cancer Res., 2003;63(20):6751-7. Species: Human Sample Type: Whole Cells Application: Flow CXCR4 function requires membrane cholesterol: implications for HIV infection. Authors: Nguyen DH, Taub D J. Immunol., 2002;168(8):4121-6. Species: Human Sample Type: Whole Cells Application: Flow
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	3T3 cells transfected with human CXCR4
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	CXCR4 is a G-protein-linked seven transmembrane spanning receptor that binds stromal cell-derived factor-1 (SDF-1). CXCR4 acts as a co-factor for T-cell tropic HIV-1 and -2 viral entry into cells. While primarily a membrane protein, CXCR4 undergoes trafficking and internalization in response to stimulation with phorbol esters and ligand (1). Cytoplasmic and nuclear localization of CXCR4 has been observed in colorectal and renal carcinomas (2,3) and it has been used as the basis of prognosis and metastatic state (3,4,5).
运输条件	Blue Ice
存放说明	-20℃
参考文献	Orsini, M.J. et al. (1999) J. Biol. Chem. 274:31076. Zagzag, D. et al. (2005) Cancer Res. 65:6178. Speetjens, F.M. et al. (2009) Cancer Microenvironment 2:1. Wang, L. et al. (2009) Oncology Reports 22:1333. Amara, S. et al. (2015) Cancer Biomark. 15:869.

参考图片

Detection of CXCR4 in Jurkat Human Cell Line by Flow Cytometry. Jurkat human acute T cell leukemia cell line was stained with Mouse Anti-Human CXCR4 Monoclonal Antibody (Catalog # MAB172, filled histogram) or isotype control antibody (Catalog # MAB004, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

CXCR4 in Human Lymph Node. CXCR4 was detected in immersion fixed paraffin-embedded sections of human lymph node using 15 µg/mL Human CXCR4 Monoclonal Antibody (Catalog # MAB172) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-AEC Cell & Tissue Staining Kit (red; Catalog # CTS003) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

CXCR4 in Human Spleen. CXCR4 was detected in immersion fixed paraffin-embedded sections of human spleen using Mouse Anti-Human CXCR4 Monoclonal Antibody (Catalog # MAB172) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Chemotaxis Induced by CXCL12/SDF‑1 alpha and Neutralization by Human CXCR4 Antibody. Recombinant Human/Feline/Rhesus Macaque CXCL12/SDF‑1 alpha (Catalog # 350-NS) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CXCR4 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human/Feline/Rhesus Macaque CXCL12/SDF‑1 alpha (1 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CXCR4 Monoclonal Antibody (Catalog # MAB172). The ND₅₀ is typically 2.5-12 µg/mL.