| 货号 | MAB6942-100 |
| 别名 | Activation-inducible TNF-related ligand; AITRLMGC138237; GITRLglucocorticoid-induced TNFR-related protein ligand; Glucocorticoid-induced TNF-related ligand; hGITRLGITR ligand; TL6AITR ligand; TNFSF18; tumor necrosis factor (ligand) superfamily, member 18; tumor necrosis factor ligand superfamily member 18 | 全称 | Glucocorticoid Induced TNF Receptor Family Related Gene Ligand |
| 反应种属 | Human |
| 应用 | Flow Cytometry,Blockade of Receptor-ligand Interaction,CyTOF-ready,ELISA Capture (Matched Antibody Pair),ELISA Detection (Matched Antibody Pair),ELISA Standard |
| 目标/特异性 | Detects human GITR Ligand/TNFSF18 in ELISAs. In sandwich immunoassays, no cross-reactivity or interference with recombinant human (rh) APRIL, rhLIGHT, rhTNF-alpha, or rhVEGI is observed. |
| 使用方法 | Flow Cytometry: 2.5 µg/106cells Blockade of Receptor-ligand Interaction: In a functional ELISA, 0.02-0.06 µg/mL of this antibody will block 50% of the binding of 10 ng/mL of biotinylated Recombinant Human GITR Ligand to immobilized Recombinant Human GITR/TNFRSF18 Fc Chimera (Catalog # 689-GR) coated at 2 µg/mL (100 µL/well). At 1 μg/mL, this antibody will block >90% of the binding. ELISA Capture (Matched Antibody Pair): 2-8 µg/mL ELISA Detection (Matched Antibody Pair): 0.5-2.0 µg/mL ELISA Standard : |
| 来源 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 8995 (Human); 240873 (Mouse) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Expression of human GITRL on myeloid dendritic cells enhances their immunostimulatory function but does not abrogate the suppressive effect of CD4+CD25+ regulatory T cells. | |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | S. frugiperda insect ovarian cell line Sf 21-derived recombinant human GITR Ligand/TNFSF18 Glu52-Ser177 Accession # Q9UNG2 |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | GITR (glucocorticoid-induced TNF receptor superfamily-related protein, also named AITR, activation-inducible TNF receptor superfamily-related protein) and GITR Ligand (GITRL) are novel members of the TNF receptor (TNFR) and TNF superfamilies (SF) that have been designated TNFRSF18 and TNFSF18, respectively. Human GITR Ligand cDNA encodes a 177 amino acid type II membrane protein. The carboxy-terminal extracellular domain shows sequence identity to TNF/TNFSF2 (21%), Fas ligand/TNFSF6 (21%), TRAIL/TNFSF10 (18%), and lymphotoxin alpha /TNFSF1 (18%). GITR Ligand is constitutively expressed in human umbilical vein endothelial cells but is not expressed in resting or stimulated T cell lines, B cell lines or peripheral blood mononuclear cells. GITR, the receptor for GITR Ligand, is expressed at low levels in peripheral blood T cells, bone marrow, thymus, spleen and lymph nodes. In contrast to mouse GITR, expression of human GITR is not induced by treatment with dexamethasone, but is up-regulated by antigen-receptor stimulation or by treatment with soluble anti-CD3 plus anti-CD28 or PMA plus ionomycin. Ligation of GITR has been found to induce nuclear factor (NF)-kappa B activation via TNF receptor-associated factor 2 and protect cells from TCR activation-induced cell death. It has been proposed that GITR Ligand and GITR may modulate T lymphocyte functions in peripheral tissues. |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 |
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