Mouse CCL24/Eotaxin-2 MAb (Clone 106521) (100 UG)【科瑞奥博】

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Mouse CCL24/Eotaxin-2 MAb (Clone 106521) (100 UG)

货号： MAB528-100 基本售价： 3985.6 元规格： 100 ug

产品信息

概述
货号	MAB528-100
别名	chemokine (C-C motif) ligand 24; Ckb-6; Eosinophil chemotactic protein 2; Eotaxin-2; member 24; MPIF-2; MPIF2Myeloid progenitor inhibitory factor 2; MPIF-2Small-inducible cytokine A24; SCYA24CK-beta-6
反应种属	Mouse
应用	Western Blot,ELISA Capture (Matched Antibody Pair),ELISA Detection (Matched Antibody Pair),ELISA Standard ,Neutralization
目标/特异性	Detects mouse CCL24/Eotaxin‑2/MPIF‑2 in Western blots. In Western blots, no cross-reactivity withrecombinant humanCCL1, 2, 3, 4, 5, 7, 8, 11, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25,28, recombinant mouse CCL1, 2, 3, 4, 6, 7, 9/10/MIP-1 gamma, 11, 12, 19, 20, 21, 22,25, 28, or recombinant rat CCL20 is observed.
使用方法	Western Blot: 1 µg/mL ELISA Capture (Matched Antibody Pair): 2-8 µg/mL ELISA Detection (Matched Antibody Pair): 0.1-0.4 µg/mL ELISA Standard : Neutralization: Measured by its ability to neutralize CCL24/Eotaxin‑2/MPIF‑2-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with mouse CCR3. The Neutralization Dose (ND₅₀) is typically 0.06-0.3 µg/mL in the presence of 20 ng/mL Recombinant Mouse CCL24/Eotaxin‑2/MPIF‑2.
来源	Monoclonal Rat IgG_2A Clone # 106521
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	6369 (Human); 56221 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. IL-23 and Th17 cells enhance Th2-cell-mediated eosinophilic airway inflammation in mice. Authors: Wakashin H, Hirose K, Maezawa Y, Kagami S, Suto A, Watanabe N, Saito Y, Hatano M, Tokuhisa T, Iwakura Y, Puccetti P, Iwamoto I, Nakajima H Am. J. Respir. Crit. Care Med., 2008;178(10):1023-32. Species: Mouse Sample Type: In Vivo Application: In vivo Intranasal mite allergen induces allergic asthma-like responses in NC/Nga mice. Authors: Shibamori M, Ogino K, Kambayashi Y, Ishiyama H Life Sci., 2005;78(9):987-94. Species: Mouse Sample Type: BALF Application: ELISA Development Pulmonary Epithelial Cell-Derived Cytokine TGF-beta1 Is a Critical Cofactor for Enhanced Innate Lymphoid Cell Function. Authors: Denney L, Byrne A, Shea T, Buckley J, Pease J, Herledan G, Walker S, Gregory L, Lloyd C Immunity, 0;43(5):945-58. Species: Mouse Sample Type: Tissue Homogenates Application: ELISA Capture
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	E. coli-derived recombinant mouse CCL24/Eotaxin‑2/MPIF‑2 Ile24-Val119 Accession # Q9JKC0
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Mouse
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	Eotaxin-2, also named myeloid progenitor inhibitory factor (MPIF-2), is a member of the CC chemokine subfamily and is designated CCL24. Eotaxin-2 is constitutively expressed in the jejunum and spleen. It can also be induced in the lung by allergen challenge and IL-4. LPS and IL-4 also differentially regulate the expression of Eotaxin-2 in monocytes and macrophages. Mouse Eotaxin-2 cDNA encodes a 119 amino acid (aa) precursor protein that shares approximately 58% aa sequence identity with human Eotaxin-2. Functionally, Eotaxin-2 is most closely related to CCL11/Eotaxin and CCL26/Eotaxin-3. The three proteins share low sequence homology but have been shown to be potent eosinophil chemoattractants that bind and activate the chemokine receptor CCR3, a receptor that is highly expressed in eosinophils. Eotaxin-2 also has the ability to suppress myeloid cell proliferation, a biological function not shared by Eotaxin.
运输条件	Blue Ice
存放说明	-20℃
参考文献	Airway Inflammation and Remodeling CC Chemokines and Receptors Chemokines and Receptors Chemokines and Receptors in Allergy and Asthma Eosinophils ICC / IHC Images: CCL24/Eotaxin-2/MPIF-2 Macrophage Activation Markers

参考图片

Chemotaxis Induced by CCL24/Eotaxin‑2 and Neutralization by Mouse CCL24/
Eotaxin‑2 Antibody. Recombinant Mouse CCL24/
Eotaxin‑2 (Catalog # 1017-MP) chemoattracts the BaF3 mouse pro‑B cell line transfected with mouse CCR3 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL24/Eotaxin‑2 (20 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse CCL24/Eotaxin‑2 Monoclonal Antibody (Catalog # MAB528). The ND₅₀ is typically 0.06‑0.3 µg/mL.