Human ALCAM MAb (Clone 105901) (100 UG)【科瑞奥博】

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Human ALCAM MAb (Clone 105901) (100 UG)

货号： MAB656-100 基本售价： 3985.6 元规格： 100 ug

产品信息

概述
货号	MAB656-100
别名	activated leucocyte cell adhesion molecule; activated leukocyte cell adhesion moleculeFLJ38514; CD166 antigen; CD166; MEMDMGC71733
全称	Activated Leukocyte Cell Adhesion Molecule
反应种属	Human
应用	Western Blot,Adhesion Blockade
目标/特异性	Detects human ALCAM/CD166 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human (rh) BCAM, recombinant mouse OCAM, rhMCAM, or rhEpCAM is observed.
使用方法	Western Blot: 2 µg/mL Adhesion Blockade: The adhesion of HuT 78 human cutaneous T cell lymphoma cells (5 x 10⁴ cells/well) to immobilized Recombinant Human CD6 Fc Chimera (Catalog # 627-CD, 10 µg/mL, 100 µL/well) was maximally inhibited (80-100%) by 5 µg/mL of the antibody.
来源	Monoclonal Mouse IgG₁ Clone # 105901
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	214 (Human); 11658 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Transcriptional and functional characterization of CD137L-dendritic cells identifies a novel dendritic cell phenotype Sci Rep, 2016;6(0):29712. Species: Human Sample Type: Whole Cells Application: Functional Assay Cocaine hijacks sigma1 receptor to initiate induction of activated leukocyte cell adhesion molecule: implication for increased monocyte adhesion and migration in the CNS. Authors: Yao H, Kim K, Duan M, Hayashi T, Guo M, Morgello S, Prat A, Wang J, Su TP, Buch S J. Neurosci., 2011;31(16):5942-55. Species: Mouse Sample Type: In Vivo Application: In vivo Wnt activity defines colon cancer stem cells and is regulated by the microenvironment. Authors: Vermeulen L, De Sousa E Melo F, van der Heijden M, Cameron K, de Jong JH, Borovski T, Tuynman JB, Todaro M, Merz C, Rodermond H, Sprick MR, Kemper K, Richel DJ, Stassi G, Medema JP Nat. Cell Biol., 2010;12(5):468-76. Species: Human Sample Type: Whole Cells Application: Flow
纯化方式	Protein A or G purified from ascites
免疫原	Mouse myeloma cell line NS0-derived recombinant human ALCAM/CD166 Trp28-Ala526 Accession # Q13740
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	ALCAM, activated leukocyte cell adhesion molecule, is a type I membrane glycoprotein and a member of the immunoglobulin supergene family. It is also known as CD166, MEMD, SC-1/DM-GRASP/BEN in the chicken, and KG-CAM in the rat. ALCAM is expressed on thymic epithelial cells, activated B and T cells, and monocytes. ALCAM can bind itself homotypically and is also capable of binding CD6, NgCAM, and other, as of yet, unidentified brain proteins. The ALCAM/CD6 interaction may be involved in T cell development and T cell regulation. Additionally, ALCAM/CD6 and ALCAM/NgCAM interactions may play roles in the nervous system. ALCAM has also been observed to be upregulated on highly metastasizing melanoma cell lines and may play a role in tumor migration. ALCAM is a 583 amino acid (aa) protein consisting of a 27 aa signal peptide, a 500 aa extracellular domain, a 24 aa transmembrane domain and a 32 aa cytoplasmic domain. The extracellular domain of ALCAM contains 5 Ig-like domains.
运输条件	Blue Ice
存放说明	-20℃
参考文献	Bowen, M.A.et al. (1995) J. Exp. Med. 181:2213. Aruffo, A.et al. (1997) Immunol. Today 18:498. Degen, W.G.et al. (1998) Am. J. Pathol. 152:805.

参考图片

Detection of Human ALCAM/CD166 by Western Blot. Western blot shows lysates of HuT 78 human cutaneous T cell lymphoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human ALCAM/CD166 Monoclonal Antibody (Catalog # MAB656) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for ALCAM/CD166 at approximately 120 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1. For best results, non-reducing conditions only are recommended.