Human CCL26/Eotaxin-3 MAb (Clone 115002) (100 UG)【科瑞奥博】

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Human CCL26/Eotaxin-3 MAb (Clone 115002) (100 UG)

货号： MAB653-100 基本售价： 3985.6 元规格： 100 ug

产品信息

概述
货号	MAB653-100
别名	CC chemokine IMAC; C-C motif chemokine 26; chemokine (C-C motif) ligand 26; chemokine N1; Eotaxin-3; IMAC; Macrophage inflammatory protein 4-alpha; MGC126714; MIP-4a; MIP-4alpha; SCYA26; SCYA26MIP-4-alpha; small inducible cytokine A26; small inducible cytokine subfamily A (Cys-Cys), member 26; Small-inducible cytokine A26; TSC-1Thymic stroma chemokine-1
反应种属	Human
应用	Western Blot,ELISA Capture (Matched Antibody Pair),ELISA Detection (Matched Antibody Pair),ELISA Standard ,Neutralization
目标/特异性	Detects human CCL26/Eotaxin-3 in ELISAs and Western blots. Does not cross-react with recombinant human (rh) CCL11/Eotaxin, recombinant mouse (rm) CCL11/Eotaxin, rhCCL24/Eotaxin-2, rmCCL24/Eotaxin-2, rhCCL3, rhCCL4, rmCCL3, rmCCL4, rhCCL5, rhCCL13, or rhCCL14.
使用方法	Western Blot: 2 µg/mL ELISA Capture (Matched Antibody Pair): 2-8 µg/mL ELISA Detection (Matched Antibody Pair): 0.1-0.4 µg/mL ELISA Standard : Neutralization: Measured by its ability to neutralize CCL26/Eotaxin‑3-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with mouse CCR3. The Neutralization Dose (ND₅₀) is typically 2-8 µg/mL in the presence of 1 µg/mL Recombinant Human CCL26/Eotaxin‑3.
来源	Monoclonal Mouse IgG₁ Clone # 115002
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	10344 (Human)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Eotaxins and CCR3 interaction regulates the Th2 environment of cutaneous T-cell lymphoma. Authors: Miyagaki T, Sugaya M, Fujita H J. Invest. Dermatol., 2010;130(9):2304-11. Species: Human Sample Type: Whole Tissue Application: IHC Paraffin-embedded Rapid chemokine secretion from endothelial cells originates from 2 distinct compartments. Authors: Oynebraten I, Bakke O, Brandtzaeg P, Johansen FE, Haraldsen G Blood, 2004;104(2):314-20. Species: Human Sample Type: Cell Culture Supernates Application: ELISA Development
纯化方式	Protein A or G purified from ascites
免疫原	E. coli-derived recombinant human CCL26/Eotaxin-3 Thr24-Leu94 Accession # Q9Y258
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	CCL26, also known as Eotaxin-3, is a CC chemokine that functions as achemoattractant for eosinophils and activated T cells throughinteractions with CCR3.
运输条件	Blue Ice
存放说明	-20℃
参考文献	CC Chemokines and Receptors Chemokines and Receptors Chemokines and Receptors in Allergy and Asthma Macrophage Activation Markers

参考图片

Detection of Human CCL26/Eotaxin‑3 by Western Blot. Western blot shows lysates of HUVEC human umbilical vein endothelial cells untreated (-) or treated (+) with
100 U/mL Recombinant Human IL‑4 (Catalog # 204-IL) for 24 hours. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human CCL26/Eotaxin‑3 Monoclonal Antibody (Catalog # MAB653) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for CCL26/
Eotaxin‑3 at approximately 12 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Chemotaxis Induced by CCL26/Eotaxin‑3 and Neutralization by Human CCL26/Eotaxin‑3 Antibody. Recombinant Human CCL26/Eotaxin‑3 (Catalog # 346‑E3) chemoattracts the BaF3 mouse pro‑B cell line transfected with mouse CCR3 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL26/Eotaxin‑3 (1 µg/mL) is neutralized (green line) by increasing concentrations of Human CCL26/Eotaxin‑3 Monoclonal Antibody (Catalog # MAB653). The ND₅₀ is typically 2-8 µg/mL.