Mouse CCL20/MIP-3 alpha MAb (Clone 114908) (100 UG)【科瑞奥博】

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Mouse CCL20/MIP-3 alpha MAb (Clone 114908) (100 UG)

货号： MAB7601-100 基本售价： 3985.6 元规格： 100 ug

产品信息

概述
货号	MAB7601-100
别名	beta chemokine exodus-1; Beta-chemokine exodus-1; CC chemokine LARC; C-C motif chemokine 20; chemokine (C-C motif) ligand 20; CKb4; exodus-1; LARC; LARCLiver and activation-regulated chemokine; MIP-3 alpha; MIP-3a; MIP-3-alpha; MIP3AMacrophage inflammatory protein 3 alpha; SCYA20Small-inducible cytokine A20; small inducible cytokine subfamily A (Cys-Cys), member 20; ST38
反应种属	Mouse
应用	ELISA Capture (Matched Antibody Pair),ELISA Detection (Matched Antibody Pair),ELISA Standard ,Neutralization
目标/特异性	Detects mouse CCL20/MIP-3 alpha in ELISAs. Does not cross-react with recombinant human (rh) CCL19, rhCCL20, recombinant mouse (rm) CCL3, 4, 9, 19, rmCXCL2, or recombinant rat CCL20.
使用方法	ELISA Capture (Matched Antibody Pair): 2-8 µg/mL ELISA Detection (Matched Antibody Pair): 0.1-0.4 µg/mL ELISA Standard : Neutralization: Measured by its ability to neutralize CCL20/MIP‑3 alpha -induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR6. The Neutralization Dose (ND₅₀) is typically 3-15 µg/mL in the presence of 20 ng/mL Recombinant Mouse CCL20/MIP‑3 alpha.
来源	Monoclonal Rat IgG₁ Clone # 114908
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	6364 (Human); 20297 (Mouse); 29538 (Rat)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. CCR6 is required for epidermal trafficking of gammadelta-T cells in an IL-23-induced model of psoriasiform dermatitis. Authors: Mabuchi T, Singh T, Takekoshi T, Jia G, Wu X, Kao M, Weiss I, Farber J, Hwang S J Invest Dermatol, 2013;133(1):164-71. Species: Mouse Sample Type: In Vivo Application: Neut Tc17 Cells Mediate Vaccine Immunity against Lethal Fungal Pneumonia in Immune Deficient Hosts Lacking CD4+ T Cells. Authors: Nanjappa SG, Heninger E, Wuthrich M, Gasper DJ, Klein BS PLoS Pathog., 2012;8(7):e1002771. Species: Mouse Sample Type: In Vivo Application: In vivo CCL20/CCR6 blockade enhances immunity to RSV by impairing recruitment of DC. Authors: Kallal LE, Schaller MA, Lindell DM, Lira SA, Lukacs NW Eur. J. Immunol., 2010;40(4):1042-52. Species: Mouse Sample Type: In Vivo Application: In vivo Rapid dendritic cell mobilization to the large intestinal epithelium is associated with resistance to Trichuris muris infection. Authors: Cruickshank SM, Deschoolmeester ML, Svensson M, Howell G, Bazakou A, Logunova L, Little MC, English N, Mack M, Grencis RK, Else KJ, Carding SR J. Immunol., 2009;182(5):3055-62. Species: Mouse Sample Type: In Vivo Application: In vivo Tumor-specific Th17-polarized cells eradicate large established melanoma. Authors: Muranski P, Boni A, Antony PA, Cassard L, Irvine KR, Kaiser A, Paulos CM, Palmer DC, Touloukian CE, Ptak K, Gattinoni L, Wrzesinski C, Hinrichs CS, Kerstann KW, Feigenbaum L, Chan CC, Restifo NP Blood, 2008;112(2):362-73. Species: Mouse Sample Type: Cell Culture Supernates Application: ELISA Development The chemokine receptor CCR6 is an important component of the innate immune response. Authors: Wen H, Hogaboam CM, Lukacs NW, Cook DN, Lira SA, Kunkel SL Eur. J. Immunol., 2007;37(9):2487-98. Species: Mouse Sample Type: In Vivo Application: In vivo
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	E. coli-derived recombinant mouse CCL20/MIP-3 alpha Ala28-Met97 Accession # O89093
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Mouse
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	MIP-3 alpha, also known as LARC (Liver and Activation-regulated Chemokine) and as Exodus, is one of many novel beta chemokines identified through bioinformatics. Mouse MIP-3 alpha cDNA encodes a 97 amino acid residue precursor protein with a 27 aa residue putative signal peptide that is predicted to be cleaved to form the 70 amino acid (aa) residue mature secreted protein. MIP-3 alpha is distantly related to other beta chemokines (20-28% aa sequence identity). Mouse MIP-3 alpha shares approximately 71% and 63% amino acid sequence homology with rat and human MIP-3 alpha, respectively. MIP-3 alpha has been shown to be expressed predominantly in lymph nodes, appendix, PBL, fetal liver, fetal lung, and epithelial cells of intestinal tissues. The expression of MIP-3 alpha is strongly up-regulated by inflammatory signals and down-regulated by the anti-inflammatory cytokine IL-10. Synthetic or recombinant MIP-3 alpha has been shown to be chemotactic for lymphocytes and dendritic cells, and inhibits proliferation of myeloid progenitors in colony formation assays. MIP-3 alpha has now been shown to be a unique functional ligand for CCR-6 (previously referred to as GPR-CY4, CKR-L3, or STRL22 orphan receptor), a chemokine receptor that is selectively and highly expressed in human dendritic cells derived from CD34⁺ cord blood precursors.
运输条件	Blue Ice
存放说明	-20℃
参考文献	Baba, M.et al. (1997) J. Biol. Chem. 272:14893. Hromas, R. et al. (1997) Blood 89:3315. Greaves, D.R. et al. (1997) J. Exp. Med. 186: 857. Tanaka, Y. et al. (1999) Eur. J. Immunol. 29:633.

参考图片

Chemotaxis Induced by CCL20/MIP‑3 alpha and Neutralization by Mouse CCL20/MIP‑3 alpha Antibody. Recombinant Mouse CCL20/MIP‑3 alpha (Catalog #
760-M3) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR6 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL20/MIP‑3 alpha (20 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse CCL20/MIP‑3 alpha Monoclonal Antibody (Catalog # MAB7601). The ND₅₀ is typically 3-15 µg/mL.