Human DC-SIGN/CD209 MAb (Clone 120507) (100 UG)【科瑞奥博】

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Human DC-SIGN/CD209 MAb (Clone 120507) (100 UG)

货号： MAB161-100 基本售价： 3985.6 元规格： 100 ug

产品信息

概述
货号	MAB161-100
简介	Clone 120507 was used by HLDA to establish CD designation
别名	CD209 antigendendritic cell-specific intracellular adhesion molecules (ICAM)-3 grabbingnon-integrin; CD209 molecule; CD209; CDSIGNHIV gpl20-binding protein; CLEC4L; CLEC4LC-type lectin domain family 4 member L; DC-SIGN1; DC-SIGN1C-type lectin domain family 4, member L; DC-SIGNMGC129965; Dendritic cell-specific ICAM-3-grabbing non-integrin 1
全称	Dendritic Cell-specific ICAM-3-grabbing Non-integrin 1
反应种属	Human
应用	Western Blot,Flow Cytometry,Immunohistochemistry,Adhesion Blockade,CyTOF-ready,Immunocytochemistry
目标/特异性	Detects human DC‑SIGN/CD209 on transfected NIH/3T3 cells and on monocyte derived dendritic cells. Does not react with parental mouse cells or irrelevant transfectants, such as human DC-SIGN2.
使用方法	Western Blot: 1 µg/mL Flow Cytometry: 2.5 µg/10⁶cells Immunohistochemistry: 8-25 µg/mL Adhesion Blockade: The adhesion of NIH-3T3 mouse embryonic fibroblast cells (5 x 10⁴ cells/well) to immobilized Recombinant Human ICAM-3/CD50 Fc Chimera (Catalog # 715-IC, 5 µg/mL, 100 µL/well) was maximally inhibited (80-100%) by 5 µg/mL of the antibody. Immunocytochemistry: 8-25 µg/mL
来源	Reconstitute at 0.5 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	30835 (Human); 170786 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. HCV RNA Activates APCs via TLR7/TLR8 While Virus Selectively Stimulates Macrophages Without Inducing Antiviral Responses Sci Rep, 2016;6(0):29447. Species: Human Sample Type: Whole Cells Application: Functional Assay Paracoccidioides brasiliensis interferes on dendritic cells maturation by inhibiting PGE2 production. Authors: Fernandes R, Bachiega T, Rodrigues D, Golim M, Dias-Melicio L, Balderramas H, Kaneno R, Soares A PLoS ONE, 2015;10(3):e0120948. Species: Human Sample Type: Whole Cells Application: Blocking Live-attenuated measles virus vaccine targets dendritic cells and macrophages in muscle of nonhuman primates. Authors: Rennick L, de Vries R, Carsillo T, Lemon K, van Amerongen G, Ludlow M, Nguyen D, Yuksel S, Verburgh R, Haddock P, McQuaid S, Duprex W, de Swart R J Virol, 2015;89(4):2192-200. Porphyromonas gingivalis evasion of autophagy and intracellular killing by human myeloid dendritic cells involves DC-SIGN-TLR2 crosstalk. Authors: El-Awady, Ahmed R, Miles, Brodie, Scisci, Elizabet, Kurago, Zoya B, Palani, Chithra, Arce, Roger M, Waller, Jennifer, Genco, Caroline, Slocum, Connie, Manning, Matthew, Schoenlein, Patricia, Cutler, Christop PLoS Pathog, 2015;10(2):e1004647. Species: Human Sample Type: Whole Cells Application: Flow In vivo targeting of human DC-SIGN drastically enhances CD8(+) T-cell-mediated protective immunity. Authors: Hesse C, Ginter W, Forg T, Mayer C, Baru A, Arnold-Schrauf C, Unger W, Kalay H, van Kooyk Y, Berod L, Sparwasser T Eur J Immunol, 2014;43(10):2543-53. Species: Mouse Sample Type: Whole Cells Application: Flow Early biodistribution and persistence of a protective live attenuated SIV vaccine elicits localised innate responses in multiple lymphoid tissues. Authors: Ferguson D, Mattiuzzo G, Ham C, Stebbings R, Li B, Rose N, Mee E, Smith D, Page M, Cranage M, Almond N, Towers G, Berry N PLoS ONE, 2014;9(8):e104390. Species: Primate - Macaca fascicularis (Crab-eating or Cynomolgus macaque) Sample Type: Whole Tissue Application: IHC Paraffin-embedded Selective susceptibility of human skin antigen presenting cells to productive dengue virus infection. Authors: Cerny D, Haniffa M, Shin A, Bigliardi P, Tan B, Lee B, Poidinger M, Tan E, Ginhoux F, Fink K PLoS Pathog, 2014;10(12):e1004548. Species: Human Sample Type: Whole Cells Application: Blocking Platelets and erythrocyte-bound platelets bind infectious HIV-1 in plasma of chronically infected patients. Authors: Beck, Zoltan, Jagodzinski, Linda L, Eller, Michael, Thelian, Doris, Matyas, Gary R, Kunz, Anjali N, Alving, Carl R PLoS ONE, 2013;8(11):e81002. Species: Human Sample Type: Whole Cells Application: Neut CLEC5A is critical for dengue virus-induced inflammasome activation in human macrophages. Authors: Wu M, Chen S, Yang A, Lin W, Lin Y, Chen N, Tsai I, Li L, Hsieh S Blood, 2013;121(1):95-106. Species: Human Sample Type: whole cells Application: Blocking Respiratory syncytial virus glycoprotein G interacts with DC-SIGN and L-SIGN to activate ERK1 and ERK2. Authors: Johnson TR, McLellan JS, Graham BS J. Virol., 2012;86(3):1339-47. Species: Human Sample Type: Whole Cells Application: Neut Retagging identifies dendritic cell-specific intercellular adhesion molecule-3 (ICAM3)-grabbing non-integrin (DC-SIGN) protein as a novel receptor for a major allergen from house dust mite. Authors: Emara M, Royer PJ, Mahdavi J J. Biol. Chem., 2012;287(8):5756-63. Species: Human Sample Type: recombinant DC-SIGN Application: ELISA Development Plasmacytoid dendritic cells infiltrate the skin in positive tuberculin skin test indurations. Authors: Bond E, Liang F, Sandgren KJ, Smed-Sorensen A, Bergman P, Brighenti S, Adams WC, Betemariam SA, Rangaka MX, Lange C, Wilkinson RJ, Andersson J, Lore K J. Invest. Dermatol., 2011;132(1):114-23. Species: Human Sample Type: Whole Tissue Application: IHC Frozen Semen clusterin is a novel DC-SIGN ligand. Authors: Sabatte J, Faigle W, Ceballos A, Morelle W, RodrÃgues CR, Lenicov FR, Thepaut M, Fieschi F, Malchiodi E, Fernandez M, Arenzana-Seisdedos F, Lortat-Jacob H, Michalski JC, Geffner J, Amigorena S J. Immunol., 2011;187(10):5299-309. Species: Human Sample Type: seminal plasma Application: Flow In situ distribution of HIV-binding CCR5 and C-type lectin receptors in the human endocervical mucosa. Authors: Hirbod T, Kaldensjo T, Broliden K PLoS ONE, 2011;6(9):e25551. Species: Human Sample Type: Whole Tissue Application: IHC Frozen Gram-positive bacteria enhance HIV-1 susceptibility in Langerhans cells, but not in dendritic cells, via Toll-like receptor activation. Authors: Ogawa Y, Kawamura T, Kimura T, Ito M, Blauvelt A, Shimada S Blood, 2009;113(21):5157-66. Species: Human Sample Type: Whole Cells Application: Flow DC-SIGN mediates cell-free infection and transmission of human T-cell lymphotropic virus type 1 by dendritic cells. Authors: Jain P, Manuel SL, Khan ZK, Ahuja J, Quann K, Wigdahl B J. Virol., 2009;83(21):10908-21. Species: Human Sample Type: Whole Cells Application: Flow A role of the Ca2+ binding site of DC-SIGN in the phagocytosis of E. coli. Authors: Iyori M, Ohtani M, Hasebe A, Totsuka Y, Shibata K Biochem. Biophys. Res. Commun., 2008;377(2):367-72. Species: Human Sample Type: Cell Lysates Application: WB Glycosylation-dependent interactions of C-type lectin DC-SIGN with colorectal tumor-associated Lewis glycans impair the function and differentiation of monocyte-derived dendritic cells. Authors: Nonaka M, Ma BY, Murai R, Nakamura N, Baba M, Kawasaki N, Hodohara K, Asano S, Kawasaki T J. Immunol., 2008;180(5):3347-56. Species: Human Sample Type: Whole Cells Application: Flow Evolving immunosuppressive microenvironment during human cervical carcinogenesis. Authors: Kobayashi A, Weinberg V, Darragh T, Smith-McCune K Mucosal Immunol, 2008;1(5):412-20. Species: Human Sample Type: Whole Tissue Application: IHC Paraffin-embedded Lewis X oligosaccharides targeting to DC-SIGN enhanced antigen-specific immune response. Authors: Wang J, Zhang Y, Wei J, Zhang X, Zhang B, Zhu Z, Zou W, Wang Y, Mou Z, Ni B, Wu Y Immunology, 2007;121(2):174-82. Species: Human Sample Type: Whole Cells Application: Neut Recombinant vesicular stomatitis viruses encoding simian immunodeficiency virus receptors target infected cells and control infection. Authors: Okuma K, Boritz E, Walker J, Sarkar A, Alexander L, Rose JK Virology, 2006;346(1):86-97. Species: Primate - Macaca mulatta (Rhesus monkey) Sample Type: Cell Lysates Application: WB Expression of DC-SIGN and DC-SIGNR on human sinusoidal endothelium: a role for capturing hepatitis C virus particles. Authors: Lai WK, Sun PJ, Zhang J, Jennings A, Lalor PF, Hubscher S, McKeating JA, Adams DH Am. J. Pathol., 2006;169(1):200-8. Species: Human Sample Type: Whole Tissue Application: IHC BCR/ABL promotes dendritic cell-mediated natural killer cell activation. Authors: Terme M, Borg C, Guilhot F, Masurier C, Flament C, Wagner EF, Caillat-Zucman S, Bernheim A, Turhan AG, Caignard A, Zitvogel L Cancer Res., 2005;65(14):6409-17. Species: Human Sample Type: Whole Cells Application: Flow DC-SIGN-mediated infectious synapse formation enhances X4 HIV-1 transmission from dendritic cells to T cells. Authors: Arrighi JF, Pion M, Garcia E, Escola JM, van Kooyk Y, Geijtenbeek TB, Piguet V J. Exp. Med., 2004;200(10):1279-88. Species: Human Sample Type: Whole Cells Application: ICC NK cell activation by dendritic cells (DCs) requires the formation of a synapse leading to IL-12 polarization in DCs. Authors: Borg C, Jalil A, Laderach D, Maruyama K, Wakasugi H, Charrier S, Ryffel B, Cambi A, Figdor C, Vainchenker W, Galy A, Caignard A, Zitvogel L Blood, 2004;104(10):3267-75. Species: Human Sample Type: Whole Cells Application: Flow Urine dendritic cells: a noninvasive probe for immune activity in bladder cancer? Authors: Beatty JD, Islam S, North ME, Knight SC, Ogden CW BJU Int., 2004;94(9):1377-83. Species: Human Sample Type: Whole Cells Application: ICC Dendritic cells in the human decidua. Authors: Gardner L, Moffett A Biol. Reprod., 2003;69(4):1438-46. Species: Human Sample Type: Whole Cells Application: ICC DC-SIGN is the major Mycobacterium tuberculosis receptor on human dendritic cells. Authors: Tailleux L, Schwartz O, Herrmann JL, Pivert E, Jackson M, Amara A, Legres L, Dreher D, Nicod LP, Gluckman JC, Lagrange PH, Gicquel B, Neyrolles O J. Exp. Med., 2003;197(1):121-7. Species: Human Sample Type: Whole Cells Application: ICC Rhesus macaque dendritic cells efficiently transmit primate lentiviruses independently of DC-SIGN. Authors: Wu L, Bashirova AA, Martin TD, Villamide L, Mehlhop E, Chertov AO, Unutmaz D, Pope M, Carrington M, KewalRamani VN Proc. Natl. Acad. Sci. U.S.A., 2002;99(3):1568-73. Species: Human Sample Type: Whole Cells Application: Flow
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	NIH-3T3 mouse embryonic fibroblast cell line transfected with human DC‑SIGN/CD209
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	Human DC-Sign (dendritic cell-specific ICAM-3 grabbing nonintegrin; also CD209) is a member of the chromosome 19 C-type lectin family that includes DC-SIGN, DC-SIGN-related protein, CD23 and LSECtin (1). DC-SIGN was initially reported to be a 46 kDa, 404 amino acid (aa) type II transmembrane protein that contained a 40 aa cytoplasmic N-terminus, a 21 aa transmembrane segment, and a 343 aa extracellular C-terminus (2). The extracellular region contains a distal, 115 aa Ca⁺⁺-dependent carbohydrate-binding lectin domain and a membrane-proximal linker segment that is composed of seven 23 aa repeats (2, 3). The lectin domain is believed to preferably bind mannose, either within the context of ICAM-3 (on T cells) or ICAM-2 (on endothelial cells) (2, 4, 5). DC-SIGN expression appears to be limited to dendritic cells (DC) and macrophages (6), and DC interaction with the ICAMs both aids DC cell trafficking and immunological synapse formation (7). Since the original report on DC-SIGN, multiple splice forms have been discovered, generating both membrane-bound and soluble forms (3). There are eight type A isoforms, all of which begin with the same 15 aa of exon 1a. Four contain the transmembrane region of exon II, and four do not (i.e., are soluble). Among these eight type A isoforms, only three retain the entire 343 aa found in the full length form described in reference #2 (the full length form is referred to as type I mDC-SIGN1A) (3). Five additional isoforms utilize an alternate start site, and these are referred to as type B isoforms. These all show a 35 aa cytoplasmic domain. One also has a transmembrane segment; four do not. Two of the five contain full, unspliced extracellular regions (3). All of this suggests enormous complexity in DC-SIGN biology. DC-SIGN is not well conserved across species. Human and mouse show little overall aa identity. In the lectin domain, however, human DC-SIGN shares 68% aa identity with mouse DC-SIGN (8). Human and rhesus monkey DC-SIGN share 91% aa identity over the entire extracellular region (8). A detailed description of the additional properties of this monoclonal antibody (MAB161) have been published (9, 10).
运输条件	Blue Ice
存放说明	-20℃
参考文献	Liu, W. et al. (2004) J. Biol. Chem. 279:18748. Curtis, B.M. et al. (1992) Proc. Natl. Acad. Sci. USA 89:8356. Mummidi, S. et al. (2001) J. Biol. Chem. 276:33196. Su, S.V. et al. (2004) J. Biol. Chem. 279:19122. Cambi, A. et al. (2005) Cell. Microbiol. 7:481. Serrano-Gomez, D. et al. (2004) J. Immunol. 173:5635. Geijtenbeek, T.B.H. and Y. van Kooyk (2003) Curr. Top. Microbiol. Immunol. 276:32. Baribaud, F. et al. (2001) J. Virol. 75:10281. Wu, L. et al. (2002) J. Virol. 76:5905. Baribaud, F. et al. (2002) J. Virol.76:9135.

参考图片

Detection of DC‑SIGN in Human DC‑SIGN Transfected 3T3 Mouse Cell Line by Flow Cytometry. Human DC‑SIGN and DC‑SIGN2 transfected 3T3 mouse embryonic fibroblast cell line were stained with Mouse Anti-Human DC‑SIGN Monoclonal Antibody (Catalog # MAB161, filled histograms) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab)₂ Secondary Antibody (Catalog # F0102B).

Detection of DC‑SIGN in Human Monocyte Derived Dendritic Cells by Flow Cytometry. Human monocyte derived dendritic cells were stained with Mouse Anti-Human DC‑SIGN Monoclonal Antibody (Catalog # MAB161) followed by PE-conjugated anti-mouse IgG (Catalog # F0102B) and Anti-Human B7-2/CD86 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB141F). Quadrant markers were set based on control antibody staining (Catalog # MAB0041).

DC-SIGN in Human Dendritic Cells. DC-SIGN was detected in immersion fixed mature human dendritic cells using Mouse Anti-Human DC-SIGN Monoclonal Antibody (Catalog # MAB161) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.