Mouse CXCL1/KC MAb (Clone 124014) (100 UG)【科瑞奥博】

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Mouse CXCL1/KC MAb (Clone 124014) (100 UG)

货号： MAB4531-100 基本售价： 3985.6 元规格： 100 ug

产品信息

概述
货号	MAB4531-100
别名	CINC-1; GRO alpha; KC; MGSA-alpha
反应种属	Mouse
应用	Western Blot,Neutralization
目标/特异性	Detects mouse CXCL1 in Western blots. Does not cross-react with recombinant human CXCL2, 3, 5, 6, 8, 9, 10, 11, 12/SDF-1 alpha,12/SDF-1 beta, 13, recombinant mouse CXCL1, 2, 6, 9, 10, 11, 12/SDF-1 alpha , 13, 15, recombinantrat (rr) CXCL2, rrCXCL3/CINC-2 alpha, rrCXCL3/CINC-2 beta, rrCXCL5, or recombinantporcine CXCL8.
使用方法	Western Blot: 1 µg/mL Neutralization: Measured by its ability to neutralize CXCL1/GRO alpha /KC/CINC‑1-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR2. The Neutralization Dose (ND₅₀) is typically 1-5 µg/mL in the presence of 30 ng/mL Recombinant Mouse CXCL1/GRO alpha /KC/CINC‑1 aa 20‑96.
来源	Monoclonal Rat IgG_2A Clone # 124014
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	2919 (Human); 14825 (Mouse); 81503 (Rat)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Pericytes support neutrophil subendothelial cell crawling and breaching of venular walls in vivo. Authors: Proebstl D, Voisin MB, Woodfin A, Whiteford J, DAcquisto F, Jones GE, Rowe D, Nourshargh S J. Exp. Med., 2012;209(6):1219-34. Species: Mouse Sample Type: Whole Tissue Application: IHC Respiratory syncytial virus reverses airway hyperresponsiveness to methacholine in ovalbumin-sensitized mice. Authors: Aeffner F, Davis I PLoS ONE, 2012;7(10):e46660. Species: Mouse Sample Type: In Vivo Application: In vivo Innate immune responses to systemic Acinetobacter baumannii infection in mice: neutrophils, but not interleukin-17, mediate host resistance. Authors: Breslow JM, Meissler JJ, Hartzell RR Infect. Immun., 2011;79(8):3317-27. Species: Mouse Sample Type: In Vivo Application: In vivo Diesel exhaust particulates exacerbate asthma-like inflammation by increasing CXC chemokines. Authors: Kim J, Natarajan S, Vaickus LJ, Bouchard JC, Beal D, Cruikshank WW, Remick DG Am. J. Pathol., 2011;179(6):2730-9. Species: Mouse Sample Type: In Vivo Application: In vivo TNF-alpha promotes LPA1- and LPA3-mediated recruitment of leukocytes in vivo through CXCR2 ligand chemokines. Authors: Zhao C, Sardella A, Chun J, Poubelle PE, Fernandes MJ, Bourgoin SG J. Lipid Res., 2011;52(7):1307-18. Species: Mouse Sample Type: In Vivo Application: In vivo Respiratory syncytial virus induces airway insensitivity to beta-agonists in BALB/c mice. Authors: Traylor ZP, Yu EN, Davis IC Am. J. Physiol. Lung Cell Mol. Physiol., 2010;298(3):L437-45. Species: Mouse Sample Type: In Vivo Application: In vivo
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	E. coli-derived recombinant mouse CXCL1/GRO alpha /KC/CINC-1 Arg20-Lys96 Accession # P12850
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Mouse
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	CXCL1/GRO alpha, also known as KC in mouse and CINC-1 in rat, is a member of the CXC family of chemokines. Human CXCL1/GRO alpha is 107 amino acids (aa) in length with a predicted molecular weight of 11 kDa. The mouse and rat orthologs share 57% and 59% amino acid sequence identity with the human protein, respectively. Genome-wide analysis suggests that the protein encoded by the mouse Cxcl3 gene is homologous to human CXCL1/GRO alpha protein. Human CXCL1/GRO alpha can be cleaved into three isoforms CXCL1/GRO alpha (aa 4-73), CXCL1/GRO alpha (aa 5-73), and CXCL1/GRO alpha (aa 6-73) which show higher activity than the full length protein. CXCL1/GRO alpha is secreted by mast cells and macrophages whereupon it acts as a chemoattractant for neutrophils.
运输条件	Blue Ice
存放说明	-20℃
参考文献	Chemokines and Receptors in Allergy and Asthma Chemokines and Receptors in Angiogenesis CXC Chemokines and Receptors ICC / IHC Images: CXCL1/GRO alpha/KC/CINC-1 Inflammatory Mediators Macrophage Activation Markers

参考图片

Chemotaxis Induced by CXCL1/KC and Neutralization by Mouse CXCL1/KC Antibody. Recombinant Mouse CXCL1/KC (Catalog # 453-KC) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CXCL1/KC (30 ng/mL) is neutralized (green line) by increasing concentrations of Mouse CXCL1/KC Monoclonal Antibody (Catalog # MAB4531). The ND₅₀ is typically 1-5 µg/mL.