Human IL-17 RA/IL-17 R MAb (Clone 133617) (100 UG)【科瑞奥博】

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Human IL-17 RA/IL-17 R MAb (Clone 133617) (100 UG)

货号： MAB177-100 基本售价： 3985.6 元规格： 100 ug

产品信息

概述
货号	MAB177-100
别名	CD217 antigen; CD217; Cdw217; CDw217interleukin 17 receptor; hIL-17R; IL-17 R; IL-17 receptor A; IL17RA; IL-17RAMGC10262; IL17Rinterleukin-17 receptor A; interleukin 17 receptor A
全称	Interleukin 17 Receptor
反应种属	Human
应用	Western Blot,Flow Cytometry,CyTOF-ready,Neutralization
目标/特异性	Detects human IL‑17 RA/IL‑17 R in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant mouse (rm) IL‑17 R, rmIL‑17B R, or recombinant human IL‑17B R is observed. In Western blots, no cross-reactivity with rmIL-17 R is observed
使用方法	Western Blot: 1 µg/mL Flow Cytometry: 2.5 µg/10⁶cells Neutralization: Measured by its ability to neutralize IL‑17/IL‑17A-induced CXCL1/GRO alpha secretion in the HT‑29 human colon adenocarcinoma cell line. The Neutralization Dose (ND₅₀) is typically 0.4-2 μg/mL in the presence of 40 ng/mL Recombinant Human IL‑17/IL‑17A.
来源	Monoclonal Mouse IgG₁ Clone # 133617
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	23765 (Human); 16172 (Mouse); 312679 (Rat)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Interleukin 17 inhibits progenitor cells in rheumatoid arthritis cartilage. Authors: Schminke B, Trautmann S, Mai B, Miosge N, Blaschke S Eur J Immunol, 2015;46(2):440-5. Species: Human Sample Type: Cell Lysates Application: WB TH17 cytokines induce human airway smooth muscle cell migration. Authors: Chang Y, Al-Alwan L, Risse PA, Roussel L, Rousseau S, Halayko AJ, Martin JG, Hamid Q, Eidelman DH J. Allergy Clin. Immunol., 2011;127(4):1046-53.e1-2. Species: Human Sample Type: Whole Cells Application: Neut IL-17C regulates the innate immune function of epithelial cells in an autocrine manner. Authors: Ramirez-Carrozzi V, Sambandam A, Luis E Nat. Immunol., 2011;12(12):1159-66. Species: Human Sample Type: Whole Cells Application: Neut T-helper cell type 2 (Th2) memory T cell-potentiating cytokine IL-25 has the potential to promote angiogenesis in asthma. Authors: Corrigan CJ, Wang W, Meng Q, Fang C, Wu H, Reay V, Lv Z, Fan Y, An Y, Wang YH, Liu YJ, Lee TH, Ying S Proc. Natl. Acad. Sci. U.S.A., 2011;108(4):1579-84. Species: Human Sample Type: Whole Cells Application: Neut Interleukin-17 and interferon-gamma are produced concomitantly by human coronary artery-infiltrating T cells and act synergistically on vascular smooth muscle cells. Authors: Eid RE, Rao DA, Zhou J, Lo SF, Ranjbaran H, Gallo A, Sokol SI, Pfau S, Pober JS, Tellides G Circulation, 2009;119(10):1424-32. Species: Human Sample Type: Whole Cells Application: Neut IL-17 induces monocyte migration in rheumatoid arthritis. Authors: Shahrara S, Pickens SR, Dorfleutner A, Pope RM J. Immunol., 2009;182(6):3884-91. Species: Human Sample Type: Whole Cells Application: Neut Functional coexpression of Interleukin (IL)-7 and its receptor (IL-7R) on Hodgkin and Reed-Sternberg cells: Involvement of IL-7 in tumor cell growth and microenvironmental interactions of Hodgkin's lymphoma. Authors: Cattaruzza L, Gloghini A, Olivo K, Di Francia R, Lorenzon D, De Filippi R, Carbone A, Colombatti A, Pinto A, Aldinucci D Int. J. Cancer, 2009;125(5):1092-101. Species: Human Sample Type: Cell Lysates Application: WB CD molecules 2006--human cell differentiation molecules. Authors: Zola H, Swart B, Banham A, Barry S, Beare A, Bensussan A, Boumsell L, D Buckley C, Buhring HJ, Clark G, Engel P, Fox D, Jin BQ, Macardle PJ, Malavasi F, Mason D, Stockinger H, Yang X J. Immunol. Methods, 2007;319(1):1-5. Species: Human Sample Type: Whole Cells Application: Flow IL-17 enhances the net angiogenic activity and in vivo growth of human non-small cell lung cancer in SCID mice through promoting CXCR-2-dependent angiogenesis. Authors: Numasaki M, Watanabe M, Suzuki T, Takahashi H, Nakamura A, McAllister F, Hishinuma T, Goto J, Lotze MT, Kolls JK, Sasaki H J. Immunol., 2005;175(9):6177-89. Species: Human Sample Type: Whole Cells Application: Flow Interleukin (IL)-4, IL-13, and IL-17A differentially affect the profibrotic and proinflammatory functions of fibrocytes from asthmatic patients. Authors: Bellini A, Marini MA, Bianchetti L, Barczyk M, Schmidt M, Mattoli S Mucosal Immunol, 0;5(2):140-9. Species: Human Sample Type: Whole Cells Application: Flow
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	Mouse myeloma cell line NS0-derived recombinant human IL‑17 RA/IL‑17 R Leu33-Trp320 Accession # Q96F46
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	Interleukin 17 (also known as CTLA-8) is a T cell-expressed pleotropic cytokine. IL-17 binds to IL-17 receptor (IL-17 R) which shares no homology with any known family of receptors. While the expression of IL-17 is restricted to activated T cells, the IL-17 R mRNA exhibits a broad tissue distribution, and has been detected in virtually all cells and tissues tested. Human IL-17 R is a 120 kDa, 866 amino acid (aa) type I membrane glycoprotein with a 293 aa extracellular domain, a 21 aa carboxy-proximal transmembrane domain, and a 525 aa cytoplasmic tail. Within the ECD, human IL-17 R shares 72% aa sequence identity with mouse and rat IL‑17 R. The signaling events of IL-17 includes activation of NF-kappa B and JNK, and require TNF receptor-associated factors 6 (TRAF6) in the signaling pathway.
运输条件	Blue Ice
存放说明	-20℃
参考文献	Yao, Z. et al. (1997) Cytokine 9:794. Schwander, R. et al. (2000) J. Exp. Med. 191:1233.

参考图片

CXCL1/GRO alpha Secretion Induced by IL‑17/IL‑17A and Neutralization by Human IL‑17 RA/IL‑17 R Antibody. Recombinant Human IL‑17/IL‑17A induces CXCL1/GRO alpha secretion in the HT‑29 human colon adenocarcinoma cell line in a dose-dependent manner (orange line), as measured by the Human CXCL1/GRO alpha DuoSet (Catalog # DY275). CXCL1/GRO alpha secretion elicited by Recombinant Human IL‑17/IL‑17A (40 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL‑17 RA/IL‑17 R Monoclonal Antibody (Catalog # MAB177). The ND₅₀ is typically 0.4-2 μg/mL.