| 货号 | MAB57541 |
| 别名 | GDF-3; growth differentiation factor 3; growth/differentiation factor 3; KFS3; MCOP7; MCOPCB6; Vgr-2 | 全称 | Growth Differentiation Factor 3 |
| 反应种属 | Human |
| 应用 | Neutralization |
| 目标/特异性 | Detects human GDF‑3 in direct ELISAs. In direct ELISAs, less than 10% cross-reactivity with recombinant human (rh) BMP-2 and rhGDF-1 is observed. |
| 使用方法 | Neutralization: Measured by its ability to neutralize GDF-3 induced Smad2 phosphorylation in the P19 mouse embryonal carcinoma cell line. 7.5-15 μg/mL of this antibody can significantly neutralize recombinant human GDF-3 (50 ng/mL) induced Smad2 phosphorylation in P19 cells. |
| 来源 | Monoclonal Mouse IgG2B Clone # 878751 |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 9573 (Human); 14562 (Mouse) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | Chinese hamster ovary cell line CHO-derived human recombinant GDF-3/BMP-2 heterodimer Ala251-Gly364 Accession # Q9NR23 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | GDF-3 (previously called Vgr-2) is a TGF-beta superfamily member belonging to the growth/differentiation factor family (1, 2). GDF-3 is expressed in undifferentiated embryonic stem (ES) cells, white adipose tissue and the brain (2-4). The 364 amino acid (aa) human GDF-3 contains a 21 aa signal sequence, a 229 aa propeptide and a 114 aa mature region that contains one potential N-glycosylation site. The mature region contains a cysteine-knot structure that is conserved throughout family members. However, it lacks the fourth cysteine which is responsible for the formation of an inter-molecular disulfide bond, so GDF-3 may exist as a non-covalent homodimer (2, 5). Mature human GDF-3 shares 83%, 83%, 91%, 92% and 93% aa sequence identity with mouse, rat, bovine, canine and equine GDF-3, respectively. Most of GDF-3 is present as the uncleaved prepro form (6). The uncleaved and the mature forms both appear to have activity, but that activity may differ (5-8). All forms can oppose BMPs. In human ES cells, inhibition of BMP-2 signaling by GDF-3 maintains pluripotency (5, 7). GDF-3 also influences early cell fate decisions; for example, deletion of mouse GDF-3 produces defects in the anterior visceral endoderm of the pre-gastrulation embryo (6-8). GDF-3 cooperates with GDF-1 in embryogenesis, and the mature protein has nodal-like activity (8, 9). Although GDF family members signal through BMP receptors (ALK-1, -2, -3 and -6), which activate Smads 1, 5 and 8, GDF-3 signaling through ALK-4 and ALK-7, which activate Smads 2 and 3, has also been reported (9, 10). In adipocytes, GDF-3 is induced by a high fat diet, promoting adipogenesis and obesity (3, 10, 11). |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
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