Mouse BMP-4 MAb (Clone 871317) (100 UG)【科瑞奥博】

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Mouse BMP-4 MAb (Clone 871317) (100 UG)

货号： MAB50201 基本售价： 3688.2 元规格： 100 ug

产品信息

概述
货号	MAB50201
别名	BMP-2B; BMP2B1; BMP2BMCOPS6; BMP-4; Bone morphogenetic protein 2B; bone morphogenetic protein 4; DVR4; OFC11; ZYME
全称	Bone Morphogenetic Protein 4
反应种属	Mouse
应用	Neutralization
目标/特异性	Detects mouse BMP-4 in direct ELISAs.
使用方法	Neutralization: Measured by its ability to neutralize BMP‑4-induced alkaline phosphatase production in the ATDC5 mouse chondrogenic cell line. Nakamura, K. et al. (1999) Exp. Cell Res. 250:351. The Neutralization Dose (ND₅₀) is typically 1.5-9 µg/mL in the presence of 20 ng/mL Recombinant Mouse BMP‑4.
来源	Monoclonal Rat IgG₁ Clone # 871317
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	652 (Human); 12159 (Mouse); 25296 (Rat); 30612 (Zebrafish)
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	Chinese hamster ovary cell line CHO-derived mature mouse BMP-4 Ser293-Arg408 Accession # P21275
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Mouse
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	BMP-4 is a TGF-beta superfamily ligand that is widely expressed from early embryogenesis through adulthood. It plays an important role in mesenchyme formation, epidermal determination, suppression of neural induction, the development of multiple organs, and tissue repair (1-5). The mouse BMP-4 precursor contains a 273 amino acid (aa) propeptide and a 116 aa mature protein (6). The propeptide is cleaved intracellularly by furin or proprotein convertase 6, enabling the 15 kDa mature BMP-4 monomer to form an active disulfide linked homodimer or heterodimer with BMP-7 (7-9). Mature mouse and human BMP-4 share 98% aa sequence identity. Mouse BMP-4 shares 85% aa sequence identity with mouse BMP-2 and 35%-54% with other mouse BMPs. Compared to BMP-4 homodimers, BMP-4/BMP-7 heterodimers exhibit a greater potency in inducing osteogenic differentiation (9). In Xenopus, the heterodimers can also induce the formation of mesoderm, whereas BMP-4 homodimers only provide ventralizing signals for existing mesoderm (10). BMP-4 signals through tetrameric complexes composed of type I (primarily Activin RIA or BMPR-IA) and type II (primarily Activin RIIA or BMPR-II) receptors (11, 12). The bioavailability of BMP-4 is regulated by its interaction with multiple proteins and glycosaminoglycans (13-15).
运输条件	Blue Ice
存放说明	-20℃
参考文献	Zhang, P. et al. (2008) Blood 111:1933. Gambaro, K. et al. (2006) Cell Death Differ. 13:1075. Simic, P. and S. Vukicevic (2005) Cytokine Growth Factor Rev. 16:299. Sadlon, T.J. et al. (2004) Stem Cells 22:457. Frank, D.B. et al. (2005) Circ. Res. 97:496. Oida, S. et al. (1995) DNA Seq. 5:273. Cui, Y. et al. (1998) EMBO J. 17:4735. Cui, Y. et al. (2001) Genes Dev. 15:2797. Aono, A. et al. (1995) Biochem. Biophys. Res. Commun. 210:670. Nishimatsu, S. and G.H. Thomsen (1998) Mech. Dev. 74:75. Chen, D. et al. (2004) Growth Factors 22:233. Lavery, K. et al. (2008) J. Biol. Chem. April 24 epub. Rosen, V. (2006) Ann. N.Y. Acad. Sci. 1068:19. Jones, C.M. and J.C. Smith (1998) Dev. Biol. 194:12. Takada, T. et al. (2003) J. Biol. Chem. 278:43229.

参考图片

Alkaline Phosphatase Production Induced by BMP-4 and Neutralization by Mouse BMP-4 Antibody.
Recombinant Mouse BMP-4 (Catalog # 5020-BP) induces alkaline phosphatase production in the ATDC5 mouse chondrogenic cell line in a dose-dependent manner (orange line). Alkaline Phosphatase Production elicited by Recombinant Mouse BMP-4 (20 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse BMP-4 Monoclonal Antibody (Catalog # MAB50201). The ND₅₀ is typically 1.5-9 µg/mL.