| 货号 | MAB6939 |
| 别名 | B cell growth factor 1; BCDF; B-cell stimulatory factor 1; BCGF1; BCGF-1; binetrakin; BSF1; BSF-1; IL-4B_cell stimulatory factor 1; interleukin 4; interleukin-4; Lymphocyte stimulatory factor 1; MGC79402; pitrakinra | 全称 | Interleukin 4 |
| 应用 | Neutralization |
| 目标/特异性 | Detects rabbit IL-4 in ELISAs. |
| 使用方法 | Neutralization: Measured by its ability to neutralize IL‑4-induced proliferation in the TF‑1 human erythroleukemic cell line. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323.The Neutralization Dose (ND50) is typically 0.25-1.25 μg/mL in the presence of 100 ng/mL Recombinant Rabbit IL‑4. |
| 来源 | Monoclonal Mouse IgG2B Clone # 855824 |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 3565 (Human); 16189 (Mouse); 287287 (Rat); 397225 (Porcine); 280824 (Bovine); 403785 (Canine); 574281 (Primate); 100302454 (Rabbit) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | E. coli-derived recombinant rabbit IL-4 Arg25-Ser147 Accession # Q9MZR8 |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Rabbit |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | Interleukin-4 (IL-4), also known as B cell-stimulatory factor-1, is a monomeric, approximately 13 kDa ‑ 18 kDa Th2 cytokine that shows pleiotropic effects during immune responses (1‑3). It is a glycosylated polypeptide that contains three intrachain disulfide bridges and adopts a bundled four alpha -helix structure (4). Rabbit IL-4 is synthesized with a 24 aa signal sequence (5). Mature rabbit IL-4 shares 47%, 56%, 39% and 40% aa sequence identity with bovine, human, mouse, and rat IL-4, respectively. Human, mouse, and rat IL-4 are species-specific in their activities (6‑8). IL-4 exerts its effects through two receptor complexes (9, 10). The type I receptor, which is expressed on hematopoietic cells, is a heterodimer of the ligand binding IL-4 R alpha and the common gamma chain (a shared subunit of the receptors for IL-2, -7, -9, -15, and -21). The type II receptor on nonhematopoietic cells consists of IL-4 R alpha and IL-13 R alpha 1. The type II receptor also transduces IL-13 mediated signals. IL-4 is primarily expressed by Th2‑biased CD4+ T cells, mast cells, basophils, and eosinophils (1, 2). It promotes cell proliferation, survival, and immunoglobulin class switch to IgG4 and IgE in human B cells, acquisition of the Th2 phenotype by naïve CD4+ T cells, priming and chemotaxis of mast cells, eosinophils, and basophils, and the proliferation and activation of epithelial cells (11 ‑ 14). IL-4 plays a dominant role in the development of allergic inflammation and asthma (13, 15). |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 |
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Proliferation Induced by IL‑4 and Neutralization by Rabbit IL‑4 Antibody. Recombinant Rabbit IL‑4 (Catalog # 6939-RB) induces proliferation in the TF‑1 human erythroleukemic cell line in a dose-dependent manner (orange line), as measured by Rezazurin (Catalog # AR002). Proliferation elicited by IL‑4 (100 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Rabbit IL‑4 Monoclonal Antibody (Catalog # MAB6939). The ND50 is typically 0.25-1.25 μg/mL. |
