| 货号 | MAB3128 |
| 别名 | atrioventricular septal defect 2; AVSD2; CIRRIN; cysteine-rich with EGF-like domain protein 1; cysteine-rich with EGF-like domains 1; DKFZp566D213 | 全称 | Cysteine-rich Protein With EGF-like Domains 1 |
| 反应种属 | Human |
| 应用 | Neutralization |
| 目标/特异性 | Detects human CRELD-1 in ELISA. |
| 使用方法 | Neutralization: Measured by its ability to neutralize CRELD1-mediated adhesion of the ATDC5 mouse chondrogenic cell line. The Neutralization Dose (ND50) is typically 0.15-0.75 μg/mL in the presence of 2 μg/mL Recombinant Human CRELD1. |
| 来源 | Monoclonal Mouse IgG2B Clone # 842314 |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 78987 (Human); 171508 (Mouse); 312638 (Rat) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant human CRELD-1 Gln30-Glu362 Accession # Q96HD1 |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | CRELD1, also known as cirrin, is an integral membrane protein that functions as a cell adhesion molecule. It is synthesized as a 420 amino acid (aa) precursor with an N-terminal signal peptide, a large extracellular domain with two EGF-like repeats, 2 transmembrane domains separated by a very short cytoplasmic loop, and a short extracellular C-terminal domain. An alternatively spliced isoform with a unique C-terminus that lacks the transmembrane domains has also been cloned. Missense mutations in CRELD1 have been associated with atrioventricular septal defects. The N-terminal extracellular domain of human and mouse CRELD1 share 92% aa sequence identity. |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
Cell Adhesion Mediated by CRELD1 and Neutralization by Human CRELD1 Antibody. Recombinant Human CRELD1 (Catalog # 3128-CR), immobilized onto a microplate, supports the adhesion of the ATDC5 mouse chondrogenic cell line in a dose-dependent manner (orange line). Adhesion elicited by Recombinant Human CRELD1 (2 μg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CRELD1 Monoclonal Antibody (Catalog # MAB3128). The ND50 is typically 0.15‑0.75 μg/mL. |
