| 货号 | MAB4675 |
| 别名 | Acidic epididymal glycoprotein homolog; acidic epididymal glycoprotein-like 1; AEGL1; AEG-related protein; ARPAEG-like protein; CRISP-1AEGL1HSCRISP1D; cysteine-rich secretory protein 1; cysteine-rich secretory protein-1 delta; HSCRISP1G; HUMARP | 全称 | Cysteine-rich Secretory Protein 1 |
| 应用 | Western Blot(1 µg/mL) Immunohistochemistry(8-25 µg/mL) |
| 目标/特异性 | Detects mouse CRISP-1 in direct ELISAs. |
| 使用方法 | Western Blot: 1 µg/mL Immunohistochemistry: 8-25 µg/mL Neutralization: Measured by its ability to neutralize CRISP‑1-inhibited proliferation in the 3A‑sub E human placenta cell line. The Neutralization Dose (ND50) is typically 10-20 µg/mL in the presence of 10 µg/mL Recombinant Mouse CRISP‑1. |
| 来源 | Monoclonal Rat IgG2B Clone # 840017 |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 167 (Human); 11571 (Mouse); 64827 (Rat) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant mouse CRISP-1 Gln20-His244 Accession # Q03401 |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | Cysteine-rich Secretory Protein 1 (CRISP‑1), also known as Acidic Epididymal Glycoprotein 1 (AEG1) and Sperm‑Coating Glycoprotein 1 (SCP1), is a 29-32 kDa (26.4 kDa predicted) protein that is involved in male reproductive biology (1, 2). CRISPs belong to the CAP superfamily of molecules that also includes several snake, insect, and lizard venom proteins (3). Structurally, CRISPs consist of an N-terminal SCP/CAP domain, a hinge region, and a Cys-rich domain with 16 invariant cysteine residues (4). CRISPs are expressed in discrete but overlapping regions of the male reproductive tract and play a role in spermatozoa adhesion with Sertoli cells and oocytes, decapacitation, and the acrosomal reaction (1). Expression patterns, genomic structure, and sequence conservation indicate that the likely ortholog of human CRISP-1 in mouse and rat is CRISP-4 (5, 6). Mature mouse CRISP-1 shares 68% amino acid (aa) sequence identity with rat CRISP-1. It shares 55%, 73%, and 43% aa sequence identity with mouse CRISP-2, -3, and -4, respectively. Rodent CRISP-1 is secreted by the epididymal epithelium and associates with the surface of spermatozoa in the epididymis where it inhibits capacitation (7-9). It is released from the sperm cell surface once capacitation begins in the female reproductive tract or in vitro (10). CRISP-1 is involved in the interaction of spermatozoa with the oocyte zona pellucida as well as in the fusion of sperm and egg (11, 12). CRISP-1 is additionally expressed in the lower medulla of hair shafts and under androgen control in the submandibular salivary glands of male mice (13-15). |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 |
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Proliferation Inhibited by CRISP‑1 and Neutralization by Mouse CRISP‑1 Antibody. Recombinant Mouse CRISP‑1 inhibits proliferation in the 3A‑sub E human placenta cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Proliferation inhibited by Recombinant Mouse CRISP‑1 (10 µg/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse CRISP‑1 Monoclonal Antibody (Catalog # MAB4675). The ND50 is typically 10-20 µg/mL. | |
Detection of Mouse CRISP‑1 by Western Blot. Western blot shows lysates of mouse epididymis tissue. PVDF membrane was probed with 1 µg/mL of Rat Anti-Mouse CRISP‑1 Monoclonal Antibody (Catalog # MAB4675) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for CRISP‑1 at approximately 27-30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. | |
CRISP‑1 in Mouse Epididymis. CRISP‑1 was detected in perfusion fixed frozen sections of mouse epididymis using Rat Anti-Mouse CRISP‑1 Monoclonal Antibody (Catalog # MAB4675) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections. |
