| 货号 | MAB6244 |
| 别名 | D3F15S2; EC 3.4.21; hepatocyte growth factor-like protein homolog; HGFL; HGFLhepatocyte growth factor-like protein; macrophage stimulating 1 (hepatocyte growth factor-like); Macrophage stimulatory protein; Macrophage-stimulating protein; MSPDNF15S2; MST1; NF15S2; SF2 | 全称 | Macrophage Stimulating Protein/Macrophage Stimulating 1 [Hepatocyte Growth Factor-like] |
| 反应种属 | Mouse |
| 应用 | Immunohistochemistry(8-25 µg/mL) |
| 目标/特异性 | Detects mouse MSP/MST1 in direct ELISAs.In direct ELISAs, no cross-reactivity withrecombinant human (rh) MSP alpha, rhMSP beta, rhproMSP, rhMSP, or recombinantmouse HGF is observed. |
| 使用方法 | Immunohistochemistry: 8-25 µg/mL Neutralization: In a functional ELISA, 10-30 ng/mL of this antibody will block 50% of the binding of 10 ng/mL of Recombinant Mouse MSP/MST1 (Catalog # 6244-MS) to immobilized Recombinant Mouse MSP R/Ron Fc Chimera (Catalog # 431-MS) coated at 0.5 μg/mL (100 µL/well). |
| 来源 | Monoclonal Rat IgG2A Clone # 757320 |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 4485 (Human); 15235 (Mouse); 24566 (Rat) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | Chinese hamster ovary cell line CHO-derived recombinant mouse MSP/MST1 Met1-Glu716 Accession # P26928 |
| 生物活性 | Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Sterile PBS to a final concentration of 0.5 mg/mL. |
| 背景 | MSP (Macrophage stimulating protein 1; also hepatocyte growth factor-like protein) is an 80-95 kDa member of the peptidase S1 family of proteins. Although it is expressed principally by hepatocytes, it can also be induced in cells such as renal tubular epithelium. MSP has multiple targets, and as such, has multiple effects. It stimulates macrophage motility and phagocytosis, promotes keratinocyte and renal tubular cell proliferation, and depresses myeloid progenitor cell replication. Mouse proMSP is 698 amino acids (aa) in length. It contains one PAN (carbohydrate-binding) site (aa 19-105), four consecutive kringle domains (110-457) and an inactive peptidase S1 region (aa 489-714). An intrachain disulfide bond exists between Cys477 and Cys593 that becomes an interchain bond following protease cleavage between Arg488 and Val489. This creates a mature heterodimer containing a 45-57 kDa alpha -chain, and a 30-35 kDa beta -chain. Mouse proMSP shares 80% and 93% aa identity with human and rat proMSP, respectively. |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 |
|
MSP/MST1 in Mouse Liver. MSP/MST1 was detected in perfusion fixed frozen sections of mouse liver using Rat Anti-Mouse MSP/MST1 Monoclonal Antibody (Catalog # MAB6244) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to hepatocytes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections. |
