Human sFRP-5 MAb (Clone 707128) (100 UG)【科瑞奥博】

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Human sFRP-5 MAb (Clone 707128) (100 UG)

货号： MAB6266 基本售价： 3688.2 元规格： 100 ug

产品信息

概述
货号	MAB6266
别名	frizzled-related protein 1b; FRP1B; FRP-1b; SARP3; SARP-3; secreted apoptosis related protein 3; secreted apoptosis-related protein 3; secreted frizzled-related protein 5; sFRP-5
全称	Secreted Frizzled Related Protein 5
反应种属	Human
应用	Neutralization
目标/特异性	Detects human sFRP-5 in direct ELISAs.
使用方法	Neutralization: Measured by its ability to neutralize sFRP-5 inhibited Topflash reporter activity in the HEK293T human embryonic kidney cell line. The Neutralization Dose (ND₅₀) is typically 2-10 μg/mL in the presence of 5 μg/mL Recombinant Human sFRP-5 and 100 ng/mL Recombinant Mouse Wnt-3a.
来源	Monoclonal Mouse IgG_2B Clone # 707128
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	6425 (Human)
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	Chinese hamster ovary cell line CHO-derived recombinant human sFRP-5 Glu30-His317 Accession # Q5T4F7
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	Secreted Frizzled Related Protein-5 (sFRP-5), also known as SARP-3, belongs to a family of Wnt‑binding proteins with homology to the ligand‑binding domain of the Frizzled receptors. sFRPs are approximately 30-35 kDa in size and contain an N‑terminal Frizzled-like domain with 10 conserved cysteines and a Netrin-like C-terminal domain (1-3). Mature human sFRP-5 shares 96% aa sequence identity with mouse and rat sFRP-2 (4). During embryonic development, sFRP-5 is expressed in the anterior visceral endoderm, neural tube, foregut epithelium, and proliferating and prehypertrophic chondrocytes (5-8). sFRP-5 activity is required for the development of foregut, liver, ventral pancreas, and somites (6, 7). In the adult, sFRP-5 is expressed in the retinal pigment epithelium and pancreas (4, 9). sFRP-5 binds and antagonizes the function of mammalian Wnt-5a and Wnt-11 as well as Xenopus Xwnt-8, resulting in an inhibition of both canonical and non-canonical Wnt signaling (7, 9, 10). sFRP-5 down-regulation is common in breast and gastric cancer cells and is correlated with poor prognosis (11-13). It functions as a tumor suppressor by inhibiting epithelial-mesenchymal transition, invasiveness, and tumorigenicity of ovarian cancer cells (14). sFRP-5 plays an important role in maintaining glucose handling and insulin sensitivity (10). It is secreted by adipocytes and is down-regulated in mouse models of obesity and type 2 diabetes (10).
运输条件	Blue Ice
存放说明	-20℃
参考文献	Bovolenta, P. et al. (2008) J. Cell Sci. 121:737. van Amerongen, R. and R. Nusse (2009) Development 136:3205. Rattner, A. et al. (1997) Proc. Natl. Acad. Sci. 94:2859. Melkonyan, H.S. et al. (1997) Proc. Natl. Acad. Sci. 94:13636. Leaf, I. et al. (2006) Genesis 44:573. Satoh, W. et al. (2008) Genesis 46:92. Li, Y. et al. (2008) Genes Dev. 22:3050. Witte, F. et al. (2009) Gene Expr. Patterns9:215. Chang, J. et al. (1999) Hum. Mol. Genet.8:575. Ouchi, N. et al. (2010) Science 329:454. Veeck, J. et al. (2008) Carcinogenesis 29:991. Zhao, C. et al. (2009) BMC Cancer 9:224. Ho, C.M. et al. (2010) Eur. J. Clin. Invest. 40:310. Su, H.Y. et al. (2010) Int. J. Cancer 127:555.

参考图片

Topflash Reporter Activity Inhibited by sFRP‑5 and Neutralization by Human sFRP‑5 Antibody. Recombinant Human sFRP‑5 (Catalog # 6266-SF) inhibits Recombinant Mouse Wnt‑3a (Catalog # 1324-WN) induced Topflash reporter activity in the HEK293T human embryonic kidney cell line in a dose-dependent manner (orange line), as measured by the Topflash Reporter Assay. Under these conditions, Topflash reporter activity inhibited by Recombinant Human sFRP‑5 (5 μg/mL) is neutralized (blue line) by increasing concentrations of Mouse Anti-Human sFRP‑5 Monoclonal Antibody (Catalog # MAB6266) in the presence of 100 ng/mL Recombinant Mouse Wnt‑3a. The ND₅₀ is typically 2‑10 μg/mL.