Human ICAM-1/CD54 MAb (Clone BBIG-I1) (100 UG)【科瑞奥博】

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Human ICAM-1/CD54 MAb (Clone BBIG-I1) (100 UG)

货号： BBA3 基本售价： 3529.5 元规格： 100 ug

产品信息

概述
货号	BBA3
别名	BB2; CD54 antigen; CD54; cell surface glycoprotein P3.58; human rhinovirus receptor; ICAM-1; intercellular adhesion molecule 1 (CD54), human rhinovirus receptor; intercellular adhesion molecule 1; Major group rhinovirus receptor; P3.58
全称	Intercellular Adhesion Molecule 1
反应种属	Human
应用	Western Blot(1 µg/mL) Immunocytochemistry(8-25 µg/mL)
目标/特异性	This antibody was selected for its ability to stain human ICAM-1/CD54 transfected COS cells. It does not stain COS cells transfected with human E-Selectin, VCAM-1, or PECAM-1.
使用方法	Western Blot: 1 µg/mL Immunoprecipitation: Davies, M.E., et al. (1991) Connect. Tissue Res. 26:207. Adhesion Blockade: The adhesion of HSB2 human peripheral blood acute lymphoblastic leukemia cells (5 x 10⁴ cells/well) to immobilized Recombinant Human ICAM-1/CD54 Fc Chimera (Catalog # 720-IC,12.5 µg/mL, 100 µL/well) was maximally inhibited (80-100%) by 10 µg/mL of the antibody. Immunocytochemistry: 8-25 µg/mL
来源	Sterile PBS to a final concentration of 0.5 mg/mL.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	3383 (Human); 15894 (Mouse); 25464 (Rat)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Role of cortactin homolog HS1 in transendothelial migration of natural killer cells. Authors: Mukherjee S, Kim J, Mooren O, Shahan S, Cohan M, Cooper J PLoS ONE, 2015;10(2):e0118153. Species: Human Sample Type: Whole Cells Application: IF Enzyme-free passage of human pluripotent stem cells by controlling divalent cations. Authors: Ohnuma, Kiyoshi, Fujiki, Ayaka, Yanagihara, Kana, Tachikawa, Saoko, Hayashi, Yohei, Ito, Yuzuru, Onuma, Yasuko, Chan, Techuan, Michiue, Tatsuo, Furue, Miho K, Asashima, Makoto Sci Rep, 2014;4(0):4646. Species: Human Sample Type: whole cells Application: IHC Uveal melanoma cells utilize a novel route for transendothelial migration. Authors: Onken, Michael, Li, Jinmei, Cooper, John A PLoS ONE, 2014;9(12):e115472. Species: Human Sample Type: Whole Cells Application: Neut Endothelial cells use dynamic actin to facilitate lymphocyte transendothelial migration and maintain the monolayer barrier. Authors: Mooren O, Li J, Nawas J, Cooper J Mol Biol Cell, 2014;25(25):4115-29. Species: Human Sample Type: Whole Cells Application: Bioassay Zoledronate attenuates angiotensin II-induced abdominal aortic aneurysm through inactivation of Rho/ROCK-dependent JNK and NF-kappaB pathway. Authors: Tsai S, Huang P, Peng Y, Chang W, Tsai H, Leu H, Chen J, Lin S Cardiovasc Res, 2013;100(3):501-10. Species: Mouse Sample Type: Tissue Lysate Application: WB GroEL1, a heat shock protein 60 of Chlamydia pneumoniae, impairs neovascularization by decreasing endothelial progenitor cell function. Authors: Lin, Yi-Wen, Huang, Chun-Yao, Chen, Yung-Hsi, Shih, Chun-Min, Tsao, Nai-Wen, Lin, Cheng-Ye, Chang, Nen-Chun, Tsai, Chien-Su, Tsai, Hsiao-Ya, Tsai, Jui-Chi, Huang, Po-Hsun, Li, Chi-Yuan, Lin, Feng-Yen PLoS ONE, 2013;8(12):e84731. Species: Human Sample Type: Cell Lysate Application: WB Macrophages induce differentiation of plasma cells through CXCL10/IP-10. J. Exp. Med., 2012;209(10):1813-23. Species: Human Sample Type: Whole Cells Application: Neut ERK5 protein promotes, whereas MEK1 protein differentially regulates, the Toll-like receptor 2 protein-dependent activation of human endothelial cells and monocytes. Authors: Wilhelmsen K, Mesa K, Lucero J, Xu F, Hellman J J Biol Chem, 2012;287(32):26478-94. Species: Human Sample Type: Whole Cells Application: Cell-based ELISA Endothelial cell substrate stiffness influences neutrophil transmigration via myosin light chain kinase-dependent cell contraction. Authors: Stroka KM, Aranda-Espinoza H Blood, 2011;118(6):1632-40. Species: Human Sample Type: Whole Cells Application: ICC IL-17 amplifies human contact hypersensitivity by licensing hapten nonspecific Th1 cells to kill autologous keratinocytes. Authors: Pennino D, Eyerich K, Scarponi C, Carbone T, Eyerich S, Nasorri F, Garcovich S, Traidl-Hoffmann C, Albanesi C, Cavani A J. Immunol., 2010;184(9):4880-8. Species: Human Sample Type: Whole Cells Application: Neut Adhesion of human haematopoietic (CD34+) stem cells to human liver compartments is integrin and CD44 dependent and modulated by CXCR3 and CXCR4. Authors: Crosby HA, Lalor PF, Ross E, Newsome PN, Adams DH J. Hepatol., 2009;51(4):734-49. Species: Human Sample Type: Whole Tissue Application: IHC SPARC is a VCAM-1 counter-ligand that mediates leukocyte transmigration. Authors: Kelly KA, Allport JR, Yu AM, Sinh S, Sage EH, Gerszten RE, Weissleder R J. Leukoc. Biol., 2007;81(3):748-56. Species: Human Sample Type: Whole Cells Application: Functional Assay Glucocorticoid-induced tumour necrosis factor receptor-related protein-mediated macrophage stimulation may induce cellular adhesion and cytokine expression in rheumatoid arthritis. Authors: Bae E, Kim WJ, Kang YM, Suk K, Koh EM, Cha HS, Ahn KS, Huh TL, Lee WH Clin. Exp. Immunol., 2007;148(3):410-8. Species: Human Sample Type: Whole Cells Application: Flow Cannabidiol attenuates high glucose-induced endothelial cell inflammatory response and barrier disruption. Authors: Rajesh M, Mukhopadhyay P, Batkai S, Hasko G, Liaudet L, Drel VR, Obrosova IG, Pacher P Am. J. Physiol. Heart Circ. Physiol., 2007;293(1):H610-9. Species: Human Sample Type: Cell Lysates Application: WB Differential survival of leukocyte subsets mediated by synovial, bone marrow, and skin fibroblasts: site-specific versus activation-dependent survival of T cells and neutrophils. Authors: Filer A, Parsonage G, Smith E, Osborne C, Thomas AM, Curnow SJ, Rainger GE, Raza K, Nash GB, Lord J, Salmon M, Buckley CD Arthritis Rheum., 2006;54(7):2096-108. Species: Human Sample Type: Whole Cells Application: Neut Migration inhibitory factor up-regulates vascular cell adhesion molecule-1 and intercellular adhesion molecule-1 via Src, PI3 kinase, and NFkappaB. Authors: Amin MA, Haas CS, Zhu K, Mansfield PJ, Kim MJ, Lackowski NP, Koch AE Blood, 2006;107(6):2252-61. Species: Human Sample Type: Cell Lysates Application: WB Proteinase-activated receptor2 agonists upregulate granulocyte colony-stimulating factor, IL-8, and VCAM-1 expression in human bronchial fibroblasts. Authors: Ramachandran R, Morice AH, Compton SJ Am. J. Respir. Cell Mol. Biol., 2006;35(1):133-41. Species: Human Sample Type: Whole Cells Application: Flow Differential effects of orbital and laminar shear stress on endothelial cells. Authors: Frattini J, Kudo FA J. Vasc. Surg., 2005;41(5):869-80. Species: Human Sample Type: Whole Cells Application: ICC Persistent cytomegalovirus infection is associated with increased expression of TGF-beta1, PDGF-AA and ICAM-1 and arterial intimal thickening in kidney allografts. Authors: Helantera I, Loginov R, Koskinen P, Tornroth T, Gronhagen-Riska C, Lautenschlager I Nephrol. Dial. Transplant., 2005;20(4):790-6. Species: Human Sample Type: Whole Tissue Application: IHC Cell expression of MMP-1 and TIMP-1 in co-cultures of human gingival fibroblasts and monocytes: the involvement of ICAM-1. Authors: Domeij H, Modeer T, Quezada HC, Yucel-Lindberg T Biochem. Biophys. Res. Commun., 2005;338(4):1825-33. Species: Human Sample Type: Whole Cells Application: Flow CC chemokines and transmigration of eosinophils in the presence of vascular cell adhesion molecule 1. Authors: Yamamoto H, Nagata M, Sakamoto Y Ann. Allergy Asthma Immunol., 2005;94(2):292-300. Species: Human Sample Type: Cell Culture Supernates Application: ELISA Development Requirement for intercellular adhesion molecule 1 and caveolae in invasion of human oral epithelial cells by Porphyromonas gingivalis. Authors: Tamai R, Asai Y, Ogawa T Infect. Immun., 2005;73(10):6290-8. Species: Human Sample Type: Whole Cells Application: Flow Apical topography and modulation of ICAM-1 expression on activated endothelium. Authors: Almenar-Queralt A, Duperray A, Miles LA, Felez J, Altieri DC Am. J. Pathol., 1995;147(5):1278-88. Species: Human Sample Type: Whole Cells Application: Flow
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	Activated HUVEC human umbilical vein endothelial cells
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Sterile PBS to a final concentration of 0.5 mg/mL.
背景	ICAM-1 is a member of the immunoglobulin superfamily whose expressionis upregulated on leukocytes, epithelial cells and resting endothelialcells in response to inflammatory signals. ICAM-1 binds the leukocyte integrins LFA-1 and Mac-1.
运输条件	Blue Ice
存放说明	-20℃
参考文献	Adhesion Molecules Adhesive Interactions in Allergy and Asthma Adipose-derived Stem Cell Markers B Cell Chemotaxis, Migration, and Adhesion Dendritic Cell Adhesion and Migration Endothelial Cell Markers Endothelial Cell Markers and Adhesion Molecules in Angiogenesis Human CD Marker Products ICC / IHC Images: ICAM-1/CD54 Immunoglobulin Superfamily CAMs Mesenchymal Stem Cell Markers Mini-review: Cytolytic Granule-mediated Apoptosis Monocyte Markers Myeloid-derived Suppressor Cells (MDSC) Neuroinflammation Plasma ICAM-1 is Correlated with Increased Risk of Cardiovascular Disease Platelets Regulatory T Cells (Tregs) T Cell Migration/Adhesion

参考图片

ICAM‑1/CD54 in HUVEC Cells. ICAM‑1/CD54 was detected in immersion fixed human umbilical vein endothelial cells (HUVECs) using Mouse Anti-Human
ICAM‑1/CD54 Monoclonal Antibody (Catalog # BBA3) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.