Human VCAM-1/CD106 MAb (Clone BBIG-V1) (100 UG)【科瑞奥博】

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Human VCAM-1/CD106 MAb (Clone BBIG-V1) (100 UG)

货号： BBA5 基本售价： 3390.7 元规格： 100 ug

产品信息

概述
货号	BBA5
别名	CD106 antigen; CD106; DKFZp779G2333; INCAM-100; L1CAM; MGC99561; vascular cell adhesion molecule 1; vascular cell adhesion protein 1; V-CAM 1; VCAM1; VCAM-1
全称	Vascular Cell Adhesion Molecule 1
反应种属	Human
应用	Western Blot(1 µg/mL) Immunoprecipitation(10 µg/mL) Immunocytochemistry(8-25 µg/mL)
目标/特异性	The antibody was screened using COS cells transfected with cDNAs for E-Selectin, VCAM-1, ICAM-1 and PECAM-1 and was shown to be specific for human VCAM-1/CD106.
使用方法	Western Blot: 1 µg/mL Immunoprecipitation: 10 µg/mL Adhesion Blockade: The adhesion of U937 human histiocytic lymphoma cells (5 x 10⁴ cells/well) to immobilized Recombinant Human VCAM-1/CD106 Fc Chimera (Catalog # 862-VC, 10 µg/mL, 100 µL/well) was maximally inhibited (80-100%) by 30 µg/mL of the antibody. Immunocytochemistry: 8-25 µg/mL
来源	Sterile PBS to a final concentration of 0.5 mg/mL.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	7412 (Human); 22329 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Uveal melanoma cells utilize a novel route for transendothelial migration. Authors: Onken, Michael, Li, Jinmei, Cooper, John A PLoS ONE, 2014;9(12):e115472. Species: Human Sample Type: Whole Cells Application: ICC GroEL1, a heat shock protein 60 of Chlamydia pneumoniae, impairs neovascularization by decreasing endothelial progenitor cell function. Authors: Lin, Yi-Wen, Huang, Chun-Yao, Chen, Yung-Hsi, Shih, Chun-Min, Tsao, Nai-Wen, Lin, Cheng-Ye, Chang, Nen-Chun, Tsai, Chien-Su, Tsai, Hsiao-Ya, Tsai, Jui-Chi, Huang, Po-Hsun, Li, Chi-Yuan, Lin, Feng-Yen PLoS ONE, 2013;8(12):e84731. Species: Human Sample Type: Cell Lysate Application: WB Macrophages induce differentiation of plasma cells through CXCL10/IP-10. J. Exp. Med., 2012;209(10):1813-23. Species: Human Sample Type: Whole Cells Application: Neut IL-15 Expression on RA Synovial Fibroblasts Promotes B Cell Survival. Authors: Benito-Miguel M, Garcia-Carmona Y, Balsa A, Bautista-Caro MB, Arroyo-Villa I, Cobo-Ibanez T, Bonilla-Hernan MG, De Ayala CP, Sanchez-Mateos P, Martin-Mola E, Miranda-Carus ME PLoS ONE, 2012;7(7):e40620. Species: Human Sample Type: Whole Cells Application: Neut Adhesion of human haematopoietic (CD34+) stem cells to human liver compartments is integrin and CD44 dependent and modulated by CXCR3 and CXCR4. Authors: Crosby HA, Lalor PF, Ross E, Newsome PN, Adams DH J. Hepatol., 2009;51(4):734-49. Species: Human Sample Type: Whole Tissue Application: IHC Reversible commitment to differentiation by human multipotent stromal cells in single-cell-derived colonies. Authors: Ylostalo J, Bazhanov N, Prockop DJ Exp. Hematol., 2008;36(10):1390-402. Species: Human Sample Type: Whole Cells Application: ICC Involvement of native TRPC3 proteins in ATP-dependent expression of VCAM-1 and monocyte adherence in coronary artery endothelial cells. Authors: Smedlund K, Vazquez G Arterioscler. Thromb. Vasc. Biol., 2008;28(11):2049-55. Species: Human Sample Type: Whole Cells Application: ELISA Development Expression and release of soluble HLA-E is an immunoregulatory feature of endothelial cell activation. Authors: Coupel S, Moreau A, Hamidou M, Horejsi V, Soulillou JP, Charreau B Blood, 2007;109(7):2806-14. Species: Human Sample Type: Cell Lysates Application: WB SPARC is a VCAM-1 counter-ligand that mediates leukocyte transmigration. Authors: Kelly KA, Allport JR, Yu AM, Sinh S, Sage EH, Gerszten RE, Weissleder R J. Leukoc. Biol., 2007;81(3):748-56. Species: Human Sample Type: Whole Cells Application: Functional Assay LIGHT up-regulated on B lymphocytes and monocytes in rheumatoid arthritis mediates cellular adhesion and metalloproteinase production by synoviocytes. Authors: Kang YM, Kim SY, Kang JH Arthritis Rheum., 2007;56(4):1106-17. Species: Human Sample Type: Cell Lysates Application: WB Cannabidiol attenuates high glucose-induced endothelial cell inflammatory response and barrier disruption. Authors: Rajesh M, Mukhopadhyay P, Batkai S, Hasko G, Liaudet L, Drel VR, Obrosova IG, Pacher P Am. J. Physiol. Heart Circ. Physiol., 2007;293(1):H610-9. Species: Human Sample Type: Cell Lysates Application: WB Proteinase-activated receptor2 agonists upregulate granulocyte colony-stimulating factor, IL-8, and VCAM-1 expression in human bronchial fibroblasts. Authors: Ramachandran R, Morice AH, Compton SJ Am. J. Respir. Cell Mol. Biol., 2006;35(1):133-41. Species: Human Sample Type: Whole Cells Application: Flow The adaptor molecule Lnk negatively regulates tumor necrosis factor-alpha-dependent VCAM-1 expression in endothelial cells through inhibition of the ERK1 and -2 pathways. Authors: Fitau J, Boulday G, Coulon F, Quillard T, Charreau B J. Biol. Chem., 2006;281(29):20148-59. Species: Human Sample Type: Cell Lysates Application: WB CC chemokines and transmigration of eosinophils in the presence of vascular cell adhesion molecule 1. Authors: Yamamoto H, Nagata M, Sakamoto Y Ann. Allergy Asthma Immunol., 2005;94(2):292-300. Species: Human Sample Type: Cell Culture Supernates Application: ELISA Development Ligation of ICAM-1 on endothelial cells leads to expression of VCAM-1 via a nuclear factor-kappaB-independent mechanism. Authors: Lawson C, Ainsworth M, Yacoub M, Rose M J. Immunol., 1999;162(5):2990-6. Species: Human Sample Type: Cell Lysates Application: WB Functions of domain 1 and 4 of vascular cell adhesion molecule-1 in alpha4 integrin-dependent adhesion under static and flow conditions are differentially regulated. Authors: Abe Y, Ballantyne CM, Smith CW J. Immunol., 1996;157(11):5061-9. Species: Human Sample Type: Cell Lysates Application: IP
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	Activated HUVEC human umbilical vein endothelial cells
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	The adhesion of U937 human histiocytic lymphoma ce
溶解方法	Sterile PBS to a final concentration of 0.5 mg/mL.
背景	Vascular cell adhesion molecule 1 (VCAM-1) is expressed on the surface of activated endothelial cells and macrophages. It binds to leukocyte integrins VLA-4 and alpha 4 beta 7 to promote leukocyte adhesion at sites of inflamed vasculature.
运输条件	Blue Ice
存放说明	-20℃
参考文献	Acute Myeloid Leukemia Adhesive Interactions in Allergy and Asthma Adipose-derived Stem Cell Markers Cancer Biomarkers Endothelial Cell Differentiation Markers Endothelial Cell Markers Endothelial Cell Markers and Adhesion Molecules in Angiogenesis Endothelial Progenitor and Endothelial Cell Markers Hematopoietic Stem Cell Markers Human CD Marker Products ICC / IHC Images: VCAM-1/CD106 Immunoglobulin Superfamily CAMs Mesenchymal Stem Cell Markers Mini-review: VCAM-1/CD106 Mouse Tissue-Resident Macrophage Markers

参考图片

VCAM‑1/CD106 in HUVEC Cells.

VCAM‑1/CD106 was detected in immersion fixed HUVEC human umbilical vein endothelial cells using Human VCAM‑1/CD106 Monoclonal Antibody (Catalog # BBA5) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.This antibody is not suitable for labeling formalin-fixed, paraffin-embedded tissue.