| 货号 | 21400-1 |
| 别名 | Fibroblast interferon; IFBIFNB; IFF; IFNB1; IFN-beta; interferon beta; interferon, beta 1, fibroblast; MGC96956 | 全称 | Interferon beta |
| 反应种属 | Human |
| 应用 | Neutralization |
| 目标/特异性 | Binds to human interferon beta with high affinity. For best results in experiments involving neutralization of the anti-viral effects of human interferon beta we recommend using Sheep Polyclonal Antibody to human interferon beta (Product 31400-1). |
| 使用方法 | Neutralization: One neutralization unit is the amount of antiserum required to neutralize one unit of human interferon beta (Hu-IFN-beta ) to a 50% endpoint. Interferon was titrated with the use of the cytopathic effect inhibition assay as described [Rubinstein, S., Familletti, P.C., and Pestka, S. (1981) "Convenient Assay for Interferons," J. Virol. 37, 755-758; Familletti, P.C., Rubinstein, S., and Pestka, S. (1981) "A Convenient and Rapid Cytopathic Effect Inhibition Assay for Interferon," in Methods in Enzymology, Vol. 78 (S. Pestka, ed.), Academic Press, New York, 387-394]. In this antiviral assay for interferon about 1 unit/ml of interferon is the quantity necessary to produce a cytopathic effect of 50%. The units are determined with respect to the international reference standard for Hu-IFN-beta provided by the National Institutes of Health [see Pestka, S. (1986) "Interferon Standards and General Abbreviations," in Methods in Enzymology (S. Pestka, ed.), Academic Press, New York 119, 14-23]. Due to the variation in ND50 (Neutralizing Dose) values based on the cell type and assay system, we recommend each user determine the neutralizing concentration of this antibody lot in their assay system. Using an A549/EMCV (cell/virus) system, we have not verified with reasonable consistency the neutralizing concentration of this antibody (the concentration required to inhibit the antiviral effect of human interferon beta by one half). |
| 来源 | Polyclonal Mouse IgG1 |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 3456 (Human); 15977 (Mouse); 24481 (Rat) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. poly(I:C) and LPS induce distinct IRF3 and NF-kappaB signaling during type-I IFN and TNF responses in human macrophages. | |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | Human interferon beta |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 背景 | Interferon-beta (IFN-beta) is a cytokine that is produced by fibroblasts and pathogen-exposed dendritic cells, macrophages, and endothelial cells. It signals through the heterodimeric IFN-alpha/beta Receptor. IFN-beta-deficient mice show increased susceptibility to experimental autoimmune encephalomyelitis (EAE), a disease model of human multiple sclerosis (MS). Furthermore, IFN-beta has been shown to suppress the Th17 cell response in both MS and EAE and has commonly been used as a treatment for MS. |
| 运输条件 | Dry Ice |
| 存放说明 | -20℃ |
| 参考文献 |
|