Mouse Oncostatin M (OSM) MAb (Clone 824157) (25 UG)【科瑞奥博】

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Mouse Oncostatin M (OSM) MAb (Clone 824157) (25 UG)

货号： MAB4951-SP 基本售价： 1467.3 元规格： 25 ug

产品信息

概述
货号	MAB4951-SP
别名	MGC20461; oncostatin M; oncostatin-M
应用	Neutralization
目标/特异性	Detects mouse Oncostatin M/OSM in direct ELISAs.
使用方法	Neutralization: Measured by its ability to neutralize Oncostatin M/OSM-induced proliferation in the NIH‑3T3 mouse embryonic fibroblast cell line. The Neutralization Dose (ND₅₀) is typically 0.2-1.2 µg/mL in the presence of 1 ng/mL Recombinant Mouse Oncostatin M/OSM.
来源	Monoclonal Rat IgG_2B Clone # 824157
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	5008 (Human); 18413 (Mouse)
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	E. coli-derived recombinant mouse Oncostatin M/OSM Ala24-Arg206 Accession # P53347
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Mouse
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	Oncostatin M (OSM) is a member of a cytokine subfamily that includes IL-6, IL-11, LIF, CNTF, and Cardiotrophin-1. These cytokines have overlapping biological functions and shared receptor components. Mouse OSM was cloned and identified as an immediate early gene induced in various myeloid and lymphoid cell lines by a subset of cytokines including IL-2, IL-3, GM-CSF, and Erythropoietin. The mouse OSM cDNA encodes a 263 amino acid residue precursor protein that shows 48% identity with human OSM. Similar to human OSM, the C-terminal region of mouse OSM contains a highly charged region. Deletion of this C-terminal region appears to be essential for the formation of biologically active mouse OSM. The biological activity of human OSM has been shown to be mediated either by the LIF/OSM receptor complex composed of gp130 and LIF R alpha or by a human OSM specific receptor composed of gp130 and OSM R alpha. It remains to be determined if the biological activities of mouse OSM can also be mediated by both receptor complexes in mouse cells.
运输条件	Blue Ice
存放说明	4℃
参考文献	Yoshimura, A. et al. (1996) EMBO J. 15:1055. Ray, P. et al. (1996) Endocrinology 137:1151. Rose, T.M. and A.G. Bruce (1994) in Guidebook to Cytokines and Their Receptors, N.A. Nicola, editor, Oxford University Press, New York, p. 127.

参考图片

Cell Proliferation Induced by Oncostatin M/OSM and Neutralization by Mouse Oncostatin M/OSM Antibody Recombinant Mouse Oncostatin M/OSM (Catalog # 495-MO) stimulates proliferation in the NIH-3T3 mouse embryonic fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse Oncostatin M/OSM (1 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse Oncostatin M/OSM Monoclonal Antibody (Catalog # MAB4951). The ND₅₀ is typically 0.2-1.2 µg/mL.