Human FGF R3 (IIIb) MAb (Clone 136315) (25 UG)
货号：
MAB1474-SP 基本售价：
1467.3 元 规格：
25 ug
产品信息
概述| 货号 | MAB1474-SP |
| 别名 | CD333; CEK; EC 2.7.10; fibroblast growth factor receptor 3; HSFGFR3EX; JTK4; JTK4thanatophoric dwarfism |
全称 | Fibroblast Growth Factor Receptor 3 | | 反应种属 | Human |
| 应用 | Neutralization |
| 目标/特异性 | Detects human FGF R3 (IIIb) in direct ELISAs. In direct ELISAs, approximately 25‑50% cross-reactivity with recombinant mouse FGF R3 (IIIb) is observed and 12% with recombinant human (rh) FGF R4 is observed and no cross-reactivity with (IIIc) isoforms of rhFGF R3 or any isoforms of rhFGF R1 or rhFGF R2 is observed. |
| 使用方法 | Neutralization: Measured by its ability to neutralize FGF R3-mediated inhibition of proliferation in the NR6R‑3T3 mouse fibroblast cell line. The Neutralization Dose (ND50) is typically 0.5-2 µg/mL in the presence of 300 ng/mL Recombinant Human FGF R3 (IIIb) Fc Chimera, 0.3 ng/mL Recombinant Human FGF acidic, and 10 µg/mL heparin.
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| 来源 | Monoclonal Mouse IgG1 Clone # 136315 |
| 产品组分 | |
性能| 供应商 | R&D Systems |
| Entrez Gene IDs | 2261 (Human); 14184 (Mouse) |
| 应用文献 |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Alternative splicing of fibroblast growth factor receptor 3 produces a secreted isoform that inhibits fibroblast growth factor-induced proliferation and is repressed in urothelial carcinoma cell lines.
Authors: Tomlinson DC, L'Hôte CG, Kennedy W, Pitt E, Knowles MA
Cancer Res., 2005;65(22):10441-9.
Species: Human
Sample Type: Cell Lysates
Application: IP |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant human FGF R3 alpha (IIIb and IIIc) |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | Fibroblast Growth Factor Receptor 3 (FGF R3) is a type I transmembrane tyrosine kinase receptor that binds FGF ligands along with heparin or heparin sulfateproteoglycans as cofactors. A segment of the membrane proximal Ig-like domain can be encoded by two different exons resulting in (IIIb) or (IIIc) isoforms. The IIIb or IIIc isoforms recognize FGF-1, -2, -4, -8b, -8e, -8f, -9,and -17b. FGF R3 plays a role in skeletal, brain, lung, intestine, kidney, and skin development. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 | - Cancer Biomarkers
- FGF Family
- FGF Ligands
- FGF Regulators
- Fibroblast Growth Factor Superfamily
- Fibroblast Growth Factors and their Receptors
- Glial Lineage Markers
- Human CD Marker Products
- ICC / IHC Images: FGF R3
- Mini-review: Fibroblast Growth Factors (FGFs)
- Multiple Myeloma
- Receptor Tyrosine Kinases (RTKs)
- Receptor Tyrosine Kinases (RTKs) in the Akt Pathway
- Receptors in the Jak/STAT Pathway
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参考图片FGF R3 Inhibition of FGF acidic-dependent Cell Proliferation and Neutralization by Human FGF R3 Antibody. Recombinant Human FGF R3 (IIIb) Fc Chimera (Catalog # 1264-FR) inhibits Recombinant Human FGF acidic (Catalog # 232‑FA) induced proliferation in the NR6R‑3T3 mouse fibroblast cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human FGF acidic (0.3 ng/mL) activity elicited by Recombinant Human FGF R3 (IIIb) Fc Chimera (300 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human FGF R3 (IIIb) Monoclonal Antibody (Catalog # MAB1474). The ND 50 is typically 0.5-2 µg/mL in the presence of heparin (10 µg/mL). |
