Human Integrin beta 1/CD29 MAb (Clone P5D2) (25 UG)【科瑞奥博】

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Human Integrin beta 1/CD29 MAb (Clone P5D2) (25 UG)

货号： MAB17781-SP 基本售价： 1467.3 元规格： 25 ug

产品信息

概述
货号	MAB17781-SP
别名	CD29 antigen; CD29; Fibronectin receptor subunit beta; FNRBVLAB; GPIIA; integrin beta-1; integrin VLA-4 beta subunit; integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includesMDF2, MSK12); ITGB1; MDF2; MSK12; very late activation protein, beta polypeptide; VLA-4 subunit beta; VLA-BETA
反应种属	Human
应用	Flow Cytometry(0.25 µg/10⁶cells) Immunocytochemistry(8-25 µg/mL)
目标/特异性	Detects human Integrin beta 1/CD29.
使用方法	Flow Cytometry: 0.25 µg/10⁶cells Immunoprecipitation: Lin, Q. et al. (2004) Biochim. Biophys. Acta 1689:175. Joneckis, C.C. et al. (1993) Blood 82:3548. Blockade of Receptor-ligand Interaction: Mukhopadhyay, N.K. et al. (2004) Ann. Thorac. Surg. 78:450. Blaschke, F. et al. (2002) Biochem. Biophys. Res. Commun. 296:890. Immunocytochemistry: 8-25 µg/mL
来源	Reconstitute at 0.5 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	3688 (Human); 16412 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. CD16 is indispensable for antibody-dependent cellular cytotoxicity by human monocytes Sci Rep, 2016;6(0):34310. Species: Human Sample Type: Whole Cells Application: Neut Mesenchymal stem cell contact promotes CCN1 splicing and transcription in myeloma cells. Authors: Dotterweich J, Ebert R, Kraus S, Tower R, Jakob F, Schutze N Cell Commun Signal, 2014;12(0):36. Species: Human Sample Type: Whole Cells Application: Neut DOCK8 regulates lymphocyte shape integrity for skin antiviral immunity. Authors: Zhang, Qian, Dove, Christop, Hor, Jyh Lian, Murdock, Heardley, Strauss-Albee, Dara M, Garcia, Jordan A, Mandl, Judith N, Grodick, Rachael, Jing, Huie, Chandler-Brown, Devon B, Lenardo, Timothy, Crawford, Greg, Matthews, Helen F, Freeman, Alexandr, Cornall, Richard, Germain, Ronald N, Mueller, Scott N, Su, Helen C J Exp Med, 2014;211(13):2549-66. Species: Human Sample Type: Whole Cells Application: Blocking Degradation of internalized alphaVbeta5 integrin is controlled by uPAR bound uPA: effect on beta1 integrin activity and alpha-SMA stress fiber assembly. Authors: Wang L, Pedroja BS, Meyers EE, Garcia AL, Twining SS, Bernstein AM PLoS ONE, 2012;7(3):e33915. Species: Human Sample Type: Whole Cells Application: Flow Epstein-Barr virus infection of polarized epithelial cells via the basolateral surface by memory B cell-mediated transfer infection. Authors: Shannon-Lowe C, Rowe M PLoS Pathog., 2011;7(5):e1001338. Species: Human Sample Type: Whole Cells Application: ICC The DSCs-expressed CD82 controls the invasiveness of trophoblast cells via integrinbeta1/MAPK/MAPK3/1 signaling pathway in human first-trimester pregnancy. Authors: Li MQ, Hou XF, Shao J, Tang CL, Li DJ Biol. Reprod., 2010;82(5):968-79. Species: Human Sample Type: Whole Cells Application: Neut Adhesion of human haematopoietic (CD34+) stem cells to human liver compartments is integrin and CD44 dependent and modulated by CXCR3 and CXCR4. Authors: Crosby HA, Lalor PF, Ross E, Newsome PN, Adams DH J. Hepatol., 2009;51(4):734-49. Species: Human Sample Type: Whole Tissue Application: IHC The chemokine receptor CX3CR1 is involved in the neural tropism and malignant behavior of pancreatic ductal adenocarcinoma. Authors: Marchesi F, Piemonti L, Fedele G, Destro A, Roncalli M, Albarello L, Doglioni C, Anselmo A, Doni A, Bianchi P, Laghi L, Malesci A, Cervo L, Malosio M, Reni M, Zerbi A, Di Carlo V, Mantovani A, Allavena P Cancer Res., 2008;68(21):9060-9. Species: Human Sample Type: Whole Cells Application: ICC
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	Human skin keratinocytes
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	The Integrin beta 1 subunit, also known as CD29, associates with at least ten different Integrin alpha subunits. It regulates not only its multiple ligands, but activates a signaling cascade in its expressing cells. CD29 is proposed to play a role in cell adhesion, apoptosis, and differentiation (1,2). Over amino acids (aa) 21-728, human and mouse share 92% aa sequence identity.
运输条件	Blue Ice
存放说明	4℃
参考文献	Barkan, D. and A.F. Chambers (2011) Clin. Cancer Res. 17:7219. Humphries, M.J. (2000) Biochem. Soc. Trans. 28:311.

参考图片

Detection of Integrin beta 1/CD29 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human Integrin beta 1/CD29 Monoclonal Antibody (Catalog # MAB17781, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).

Integrin beta 1/CD29 in human PBMCs. Integrin beta 1/CD29 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Mouse Anti-Human Integrin beta 1/CD29 Monoclonal Antibody (Catalog # MAB17781) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.