| 货号 | MAB2144-SP |
| 别名 | Epiligrin; LAMA3; Laminin-5 |
| 反应种属 | Human |
| 应用 | Immunohistochemistry(8-25 µg/mL) Immunocytochemistry(8-25 µg/mL) |
| 目标/特异性 | Detects human Laminin alpha 3/Laminin‑5 in Western blots. |
| 使用方法 | Western Blot: Wayner, E.A. (1993) J. Cell Biol. 121(5):1141; Gallatin, W.M. et al. (1989) Proc. Natl. Acad. Sci. USA 86(12):4654. This application was not tested by R&D Systems. Immunohistochemistry: 8-25 µg/mL Immunoprecipitation: Gallatin, W.M. et al. (1989) Proc. Natl. Acad. Sci. 86:4654. Adhesion Blockade: Gonzales, M. et al. (1999) Mol. Biol. Cell 10:259. Immunocytochemistry: 8-25 µg/mL |
| 来源 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 3909 (Human) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Elastin fragments drive disease progression in a murine model of emphysema. | |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | Human keratinocytes |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | Laminins are heterotrimeric, noncollagenous glycoproteins composed of alpha, beta and gamma chains. Through interactions with Integrins, Dystroglycan and other receptors, Laminins contribute to cell differentiation, cell shape and migration, and maintenance of tissue phenotypes and survival (1). Laminin-5, also known as Epiligrin, consists of an alpha 3, beta 3, and gamma 2 chain. It is abundant in transitional epithelium, stratified squamous epithelia, lung mucosa and other epithelial glands and plays an important role in the initiation and maintenance of epithelial cell anchorage to the underlying connective tissue (2, 3). |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Laminin alpha 3/Laminin-5 in MCF 10A Human Cell Line. Laminin alpha 3/Laminin-5 was detected in immersion fixed MCF 10A human breast epithelial cell line using 10 µg/mL Mouse Anti-Human Laminin alpha 3/Laminin-5 Monoclonal Antibody (Catalog # MAB2144) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips. | |
Laminin alpha 3/Laminin‑5 in Human Skin. Laminin alpha 3/Laminin‑5 was detected in immersion fixed paraffin-embedded sections of human skin using Mouse Anti-Human Laminin alpha 3/Laminin‑5 Monoclonal Antibody (Catalog # MAB2144) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific labeling was localized to cell in the epiderimis. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. |
