Mouse IL-12/IL-23 p40 MAb (Clone C17.8) (25 UG)【科瑞奥博】

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Mouse IL-12/IL-23 p40 MAb (Clone C17.8) (25 UG)

货号： MAB4991-SP 基本售价： 1467.3 元规格： 25 ug

产品信息

概述
货号	MAB4991-SP
别名	CLMF2; IL12B; IL-12B; NKSF2
全称	Interleukin 12/Interleukin 23 p40
反应种属	Mouse
应用	Western Blot(1 µg/mL) ELISA Capture (Matched Antibody Pair)(2-8 µg/mL ) ELISA Detection (Matched Antibody Pair)(0.1-0.4 µg/mL ) ELISA Standard ( )
目标/特异性	Detects the mouse IL-12/IL-23 p40 subunit. Polymorphisms exist in the mouse p40 sequences [Ymer, S. et al. (2002) Genes and Immunity 3:151.].
使用方法	Western Blot: 1 µg/mL ELISA Capture (Matched Antibody Pair): 2-8 µg/mL ELISA Detection (Matched Antibody Pair): 0.1-0.4 µg/mL ELISA Standard : Neutralization: Measured by its ability to neutralize IL‑12-induced proliferation in PHA-activated mouse splenocytes. The Neutralization Dose (ND₅₀) is typically 0.04-0.3 µg/mL in the presence of 1 ng/mL Recombinant Mouse IL‑12.
来源	Monoclonal Rat IgG_2A Clone # C17.8
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	3593 (Human); 16160 (Mouse); 64546 (Rat); 403976 (Canine); 493741 (Feline)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Co-adjuvant effects of retinoic acid and IL-15 induce inflammatory immunity to dietary antigens. Authors: DePaolo RW, Abadie V, Tang F Nature, 2011;471(7337):220-4. Species: Mouse Sample Type: In Vivo Application: In vivo The pro-Th2 cytokine IL-33 directly interacts with invariant NKT and NK cells to induce IFN-gamma production. Authors: Bourgeois E, Van LP, Samson M, Diem S, Barra A, Roga S, Gombert JM, Schneider E, Dy M, Gourdy P, Girard JP, Herbelin A Eur. J. Immunol., 2009;39(4):1046-55. Species: Mouse Sample Type: Whole Cells Application: Neut TSU68 prevents liver metastasis of colon cancer xenografts by modulating the premetastatic niche. Authors: Yamamoto M, Kikuchi H, Ohta M, Kawabata T, Hiramatsu Y, Kondo K, Baba M, Kamiya K, Tanaka T, Kitagawa M, Konno H Cancer Res., 2008;68(23):9754-62. Species: Mouse Sample Type: In Vivo Application: In vivo Glechoma hederacea inhibits inflammatory mediator release in IFN-gamma and LPS-stimulated mouse peritoneal macrophages. Authors: An HJ, Jeong HJ, Um JY, Kim HM, Hong SH J Ethnopharmacol, 2006;106(3):418-24. Species: Mouse Sample Type: Cell Culture Supernates Application: ELISA Development 17beta-estradiol (E2) modulates cytokine and chemokine expression in human monocyte-derived dendritic cells. Authors: Bengtsson AK, Ryan EJ, Giordano D, Magaletti DM, Clark EA Blood, 2004;104(5):1404-10. Species: Human Sample Type: Cell Culture Supernates Application: ELISA Development Novel p19 protein engages IL-12p40 to form a cytokine, IL-23, with biological activities similar as well as distinct from IL-12. Authors: Oppmann, B, Lesley, R, Blom, B, Timans, J C, Xu, Y, Hunte, B, Vega, F, Yu, N, Wang, J, Singh, K, Zonin, F, Vaisberg, E, Churakova, T, Liu, M, Gorman, D, Wagner, J, Zurawski, S, Liu, Y, Abrams, J S, Moore, K W, Rennick, D, de Waal-Malefyt, R, Hannum, C, Bazan, J F, Kastelein, R A Immunity, 2000;13(5):715-25. Species: Mouse Sample Type: Whole Cells Application: Flow
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	Recombinant mouse IL-12 p70 heterodimer
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Mouse
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	Interleukin 12, also known as natural killer cell stimulatory factor (NKSF) or cytotoxic lymphocyte maturation factor (CLMF), is a pleiotropic cytokine originally identified in the medium of activated human B lymphoblastoid cell lines. IL-12 has multiple effects on T cells and NK cells and is a key mediator in the development of Th1 cells. IL-12 is a heterodimeric cytokine containing two disulfide-linked subunits, p35 and p40. Human and mouse IL-12 share 70% and 60% amino acid sequence identity in their p40 and p35 subunits, respectively. Although mouse IL-12 is active on human or mouse IL-12 responsive cells, human IL-12 is not active on mouse cells. The disulfide-linked mouse p40 homodimer can bind to IL-12 receptors and is an antagonist of IL-12 activities in vitro. The mouse p40 monomer is at least ten times less active than the homodimer as an IL-12 antagonist. At the present time, the existence and the physiological role of mouse p40 homodimer in vivo remains to be determined.
运输条件	Blue Ice
存放说明	4℃
参考文献	Cytokines and Receptors in Allergy and Asthma Cytokines and Receptors in Angiogenesis gamma delta T Cells IL-10 and IL-12 Influence in Dendritic Cell Maturation IL-12 Family IL-12: Cell Protection from UVB Th1 Cells Th17 Cells

参考图片

Cell Proliferation Induced by IL‑12 and Neutralization by Mouse IL‑12/IL‑23 p40 Antibody. Recombinant Mouse IL‑12 (Catalog # 419-ML) stimulates proliferation in PHA-activated mouse splenocytes in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Mouse IL‑12 (1 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse IL‑12/IL‑23 p40 Monoclonal Antibody (Catalog # MAB4991). The ND₅₀ is typically
0.04-0.3 µg/mL.