| 货号 | MAB2549-SP |
| 别名 | Laminin1; Laminin-1 |
| 反应种属 | Mouse |
| 应用 | Western Blot,Immunohistochemistry,Adhesion Blockade |
| 目标/特异性 | Reacts with mouse Laminin-1 A and B chains on the long arm below the cross region. |
| 使用方法 | Western Blot: Skubitz, A.P. et al. (1987) Exp. Cell Res. 173:349. This application was not tested by R&D Systems. Immunohistochemistry: Skubitz, A.P. et al. (1987) Exp. Cell Res. 173:349. Adhesion Blockade: Skubitz, A.P. et al. (1987) Exp. Cell Res. 173:349. |
| 来源 | Monoclonal Rat IgG1 Clone # AL-2 |
| 产品组分 |
| 供应商 | R&D Systems |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | Purified fragment of chymotrypsin-digested mouse EHS tumor-derived Laminin-1 |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | Laminins are heterotrimeric, noncollagenous glycoproteins composed of alpha, beta and gamma chains. Through interactions with integrins, dystroglycan, and other receptors, laminins contribute to cell differentation, cell shape and migration, maintenance of tissue phenotypes, and survival. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Laminin‑1 in Mouse Embryo. Laminin‑1 was detected in immersion fixed frozen sections of embryonic mouse parietal endoderm (E10.5) using Mouse Laminin‑1 Monoclonal Antibody (Catalog # MAB2549) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 637-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL014) and counterstained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections. |
