| 货号 | MAB5821-SP |
| 别名 | Cytokine Zcyto18; IL-10-related T-cell-derived inducible factor; IL-21; IL-22IL21; IL-D110; IL-TIF; ILTIFIL-10-related T-cell-derived-inducible factor; IL-TIFMGC79382; interleukin 21; interleukin 22; interleukin-22; MGC79384; TIFa; TIFIL-23; zcyto18 | 全称 | Interleukin 22 |
| 反应种属 | Mouse |
| 应用 | Neutralization |
| 目标/特异性 | Detects mouse IL-22 in direct ELISAs. |
| 使用方法 | Neutralization: Measured by its ability to neutralize IL‑22-induced IL-10 secretion in the COLO 205 human colorectal adenocarcinoma cell line. The Neutralization Dose (ND50) is typically 0.5-6 µg/mL in the presence of 500 pg/mL Recombinant Mouse IL‑22. |
| 来源 | Monoclonal Rat IgG2A Clone # 924324 |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 50616 (Human); 50929 (Mouse); 500836 (Rat) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | E. coli-derived recombinant mouse IL-22 Leu34-Val179 Accession # Q9JJY9 |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | Interleukin-22 (IL-22), also known as IL-10-related T cell-derived inducible factor (IL-TIF) was initially identified as a gene induced by IL-9 in mouse T cells and mast cells. Mouse IL-22 cDNA encodes a 179 amino acid (aa) residue protein with a putative 33 aa signal peptide that is cleaved to generate a 147 aa mature protein that shares approximately 79% and 22% aa sequence identity with human IL-22 and IL-10, respectively. The mouse IL-22 gene is localized to chromosome 10. Although it exists as a single copy gene in many mouse strains, the IL-22 gene is duplicated in some mouse strains including C57B1/6, FVB and 129. The two mouse genes designated IL-TIF alpha and IL-TIF beta, share greater than 98% sequence homology in their coding region. IL-22 has been shown to activate STAT-1 and STAT-3 in several hepatoma cell lines and upregulate the production of acute phase proteins. IL-22 is produced by normal mouse T cells upon Con A activation. Mouse IL-22 expression is also induced in various organs upon lipopolysaccharide injection, suggesting that IL-22 may be involved in inflammatory responses. The functional IL-22 receptor complex consists of two receptor subunits, IL-22R (previously an orphan receptor named CRF2-9) and IL-10R beta (previously known as CRF2-4), belonging to the class II cytokine receptor family. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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IL-10 Secretion Induced by IL-22 and Neutralization by Mouse IL-22 Antibody. Recombinant Mouse IL‑22 stimulates IL-10 secretion in the COLO 205 human colorectal adenocarcinoma cell line in a dose-dependent manner (orange line), as measured by the Human IL-10 DuoSet ELISA Development Kit (Catalog # DY217B). IL-10 Secretion elicited by Recombinant Mouse IL‑22 (500 pg/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse IL‑22 Monoclonal Antibody (Catalog # MAB5821). The ND50 is typically 0.5-6 µg/mL. |
