| 货号 | MAB80841-SP |
| 别名 | HERV-H LTR-associating 2; HERV-H LTR-associating protein 2; HHLA2; Human endogenous retrovirus-H long terminal repeat-associating protein 2 | 全称 | B7 Homolog 7 |
| 应用 | Immunocytochemistry(8-25 µg/mL) |
| 目标/特异性 | Detects human B7-H7/HHLA2 in direct ELISAs. |
| 使用方法 | Blockade of Receptor-ligand Interaction: In a functional ELISA, 100-300 ng/mL of Mouse anti-Human B7-H7/HHLA2 Antibody (Catalog # MAB80841) will block 50% of the binding of 500 ng/mL of Recombinant Human B7-H7/HHLA2 Fc Chimera (Catalog # 8084-B7) to immobilized Recombinant Human CD28H/IGPR-I Fc Chimera (Catalog # 8316-TR) coated at 125 ng/mL (100 µL/well). At 1.25 μg/mL, this antibody will block >90% of the binding. Immunocytochemistry: 8-25 µg/mL |
| 来源 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 11148 (Human) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | Human embryonic kidney cell line HEK293-derived human B7-H7/HHLA2 Met1-Asn344 Accession # Q9UM44 |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | B7-H7, also known as HHLA2 (HERV-H LTR-associating 2), is a member of the B7 family of immune regulatory proteins (1, 2). Mature human B7-H7 consists of a 322 amino acid (aa) extracellular domain (ECD) with three immunoglobulin-like domains, a 21 aa transmembrane segment, and a 49 aa cytoplasmic domain (3-5). B7‑H7 is constitutively expressed on monocytes and is up-regulated by LPS and IFN-gamma stimulation. It is expressed on LPS/IFN-gamma treated B cells but not on resting B cells (5). B7-H7 binds to cell surface determinants on resting and mature T cells, B cells, and monocytes as well as on immature and mature dendritic cells (5). Soluble B7-H7 inhibits the proliferation of activated CD4+ and CD8+ T cells and their production of IFN-gamma, TNF-alpha, IL-5, IL-10, IL-13, IL-17A, and IL-22 (5). |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 |
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CD28H Binding to B7-H7-transfected HEK293 Human Cell Line is Blocked by Human B7-H7 Antibody. In a functional flow cytometry test, biotinylated recombinant human CD28H/IGPR-I (10 ng/mL) binds to HEK293 human embryonic kidney cell line transfected with human B7-H7 (black dotted line). Binding is completely blocked (orange histogram) by 2.5 μg/mL of Mouse Anti-Human B7-H7 Monoclonal Antibody (Catalog # MAB80841). Mouse IgG1 Isotype Control (Catalog # MAB002) at 2.5 μg/mL was used as a control (blue line). Cells were stained with Streptavidin-APC (Catalog # F0050). | |
B7-H7 Binding to CD28H Blocked by Human B7-H7 Antibody. In a Surface Plasmon Resonance test, Recombinant Human CD28H/IGPR-I Fc Chimera (Catalog # 8316-TR), coated at 10 ng/mL binds to Recombinant Human B7-H7/HHLA2 Fc Chimera (Catalog # 8084-B7, 5 μg/mL) (blue line). Binding is completely blocked (red) by 50 μg/mL of Mouse Anti-Human B7-H7 Monoclonal Antibody (Catalog # MAB80841). Mouse IgG1 isotype (Catalog # MAB002) and irrelevant mouse monoclonal antibody were used as controls and did not affect BD28H binding to B7-H7 (not shown). | |
B7-H7/HHLA2 in Human PBMCs. B7-H7/HHLA2 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Mouse Anti-Human B7-H7/HHLA2 Monoclonal Antibody (Catalog # MAB80841) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells. |
