| 货号 | MAB8296-SP |
| 别名 | FLJ22573; TMEM149; transmembrane protein 149; U2 small nuclear RNA auxiliary factor 1-like 4; U2(RNU2) small nuclear RNA auxiliary factor 1-like 4; U2AF1L4 | 全称 | IGF-like Family Receptor 1 |
| 反应种属 | Human |
| 应用 | Flow Cytometry(0.25 µg/106cells) |
| 目标/特异性 | Detects human IGFLR1in direct ELISAs. |
| 使用方法 | Flow Cytometry: 0.25 µg/106cells Blockade of Receptor-ligand Interaction: In a functional ELISA, 0.05-0.3 μg/mL of this antibody will block 50% of the binding of 10 ng/mL of rhIGFL-1 to immobilized rhIGFLR1 coated at 100 ng/mL (100 μL/well). At 10 μg/mL, this antibody will block >90% of the binding. |
| 来源 | Monoclonal Mouse IgG1 Clone # 905338 |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 79713 (Human); 101883 (Mouse); 499126 (Rat) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | Human embryonic kidney cell line HEK293-derived recombinant human IGFLR1 Met1-Pro163 Accession # Q9H665 |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | Insulin Growth Factor-like Family Receptor 1 (IGFLR1) is a 355 amino acid (aa) type 1a transmembrane protein that was identified in a screen for binding partners of human IGFL-1 (1). Mature human IGFLR1 consists of an extracellular domain (ECD) with two putative cysteine-rich domains (CRDs), a transmembrane region, and a cytoplasmic domain (1). Its structure has similarities to TNF receptor family members (1). Over the first 163 aa, human IGFLR1 shares 61% and 59% aa sequence identity with mouse and rat IGFLR1, respectively. In mice, IGFLR1 is expressed primarily on T cells and, similar to the human proteins, mouse IGFLR1 binds the mouse IGFL protein (1). Human IGFL-1 expression is enhanced by TNF-alpha treatment and was shown to be up-regulated in human psoriatic skin samples, suggesting that IGFLR1 may have a role during skin inflammation (1). |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of IGFLR1 in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD3 epsilon PE‑conjugated Monoclonal Antibody (Catalog # FAB100P) and either (A) Mouse Anti-Human IGFLR1 Monoclonal Antibody (Catalog # MAB8296) or (B) Mouse IgG1 Isotype Control (Catalog # MAB002) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). |
