| 货号 | MAB8241-SP |
| 别名 | Apoptosis regulator Bcl-W; BCL2L2; Bcl2-L-2; BCL2-like 2; BCL-W; BCLWbcl-2-like protein 2; KIAA0271BCL2-L-2 | 全称 | B Cell Lymphoma/Leukemia w |
| 反应种属 | Human/Mouse |
| 应用 | Neutralization |
| 目标/特异性 | Detects human and mouse Bcl‑w. Neutralizes recombinant human and mouse Bcl-w, but not recombinant human Bcl‑2 or Bcl‑x. |
| 使用方法 | Neutralization |
| 来源 | Monoclonal Mouse IgG1 Clone # 90336 |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 599 (Human); 12050 (Mouse) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | E. coli-derived recombinant human Bcl-w Met1-Thr172 |
| 生物活性 | Human, Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | Bcl-w is a member of the Bcl-2 family of proteins that regulates outer mitochondrial membrane permeability. Bcl-w is an anti-apoptotic member that prevents release of cytochrome c from the mitochondria intermembrane space into the cytosol. Bcl-w is required for normal sperm maturation. Natural Bcl-w contains a carboxyl-terminal mitochondria targeting sequence. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Neutralization Cytochrome c released from enriched mouse liver mitochondria was quantified with the Rat/Mouse Cytochrome c ELISA (Catalog # MCTC0). X ± SEM, n=3. Total: Cytochrome c released from mouse liver mitochondria with Triton®X-100 Untreated: Cytochrome c released from untreated mitochondria BID: Cytochrome c released from mitochondria when incubated with casp-8 cleaved human BID (Catalog # 882-B8) BID + Bcl-w: Cytochrome c released from mitochondria when incubated with casp-8 cleaved human BID and human Bcl-w (Catalog # 824-BW) BID + Bcl-w + MAB8241: Cytochrome c released from mitochondria when incubated with casp-8 cleaved human BID, human Bcl-w and Bcl-w neutralizing antibody |
