| 货号 | MAB7796-SP |
| 别名 | matrix metallopeptidase 17 (membrane-inserted); matrix metalloproteinase 17 (membrane-inserted); matrix metalloproteinase-17; membrane-type matrix metalloproteinase 4; membrane-type-4 matrix metalloproteinase; MMP-17; MT4MMP; MT4-MMP; MT-MMP 4; MTMMP4 | 全称 | Matrix Metalloproteinase 17 |
| 反应种属 | Human |
| 应用 | Neutralization |
| 目标/特异性 | Detects human MMP-17 in direct ELISAs. In direct ELISAs, no cross-reactivitywith recombinant human MMP-24 is observed. |
| 使用方法 | Neutralization: Measured by itsability to neutralize Recombinant Human MMP-17 (1.0 μg/mL, Catalog # 7796-MP) cleavage of the fluorogenic peptide substrate Mca-PLAQAV-Dpa-RSSSR-NH2 (10μM, Catalog # ES003). The Neutralization Dose (ND50) is < 9.0 μg/mL. |
| 来源 | Monoclonal Mouse IgG2A Clone # 823511 |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 4326 (Human); 23948 (Mouse); 288626 (Rat) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant human MMP-17 Pro42-Ala534 Accession # Q9ULZ9 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Sterile PBS to a final concentration of 0.5 mg/mL. |
| 背景 | Matrix metalloproteinase 17 (MMP‑17) is a zinc metalloproteinase that is an integral membrane protein (1). It belongs to the membrane‑type MMP (MT‑MMP) subfamily, and is also known as MT4‑MMP. It is associated with the cell membrane by a glycosylphosphatidylinositol (GPI) anchor (1). It has the potential to function as a pro‑tumor necrosis factor‑ alpha (TNF‑ alpha ) convertase (2). Unlike MMP‑14 (MT1‑MMP), it is a poor activator of pro‑MMP‑2. MMP‑17 is relatively poor at digesting components of the extracellular matrix but does cleave fibrinogen and fibrin (2). MMP‑17 is known to activate the aggrecanase ADAMTS‑4 through proteolysis (3). MMP‑17 is expressed in many tissues with the highest levels seen in the brain, colon, ovary, testis, and kidney papilla (4). It has been detected in several human cancers, including gliomas, prostate carcinomas, and breast carcinomas (5). |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Neutralization of Human MMP‑17 by Human MMP-17 Antibody. The cleavage of Mca‑PLAQAV‑Dpa‑RSSSR‑NH2 (10 μM, Catalog # ES003) by Recombinant Human MMP-17 (1.0 ug/mL, Catalog # 7796-MP) is measured after it has been preincubated with increasing concentrations of Mouse Anti-Human MMP-17 Monoclonal Antibody (Catalog # MAB7796). The ND50 is typically 9.0 μg/mL. |
